Lab-Scale production of Bacillus atrophaeus' spores by solid state fermentation in fifferent types of bioreactors

• Autor(a) principal: Sella,Sandra Regina Barroso Ruiz
• Data de Publicação: 2009
• Outros Autores: Guizelini,Belquis Palácio, Vandenberghe,Luciana Porto de Souza, Medeiros,Adriane Bianchi Pedroni, Soccol,Carlos Ricardo
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Archives of Biology and Technology
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700021
• topic: Bacillus atrophaeus; spores; solid state fermentation; column bioreactor; biofilm

Studies were conducted to evaluate Bacillus atrophaeus spores' production by solid-state fermentation (SSF) using sugarcane bagasse as support and soybean molasses as substrate at lab-scale in column bioreactors (forced aeration), plastic bags and Erlenmeyer flasks (aeration by diffusion). Different moisture contents (84%, 86% and 88%; 89%, 91% and 93%) and aeration rates (30mL/min, 45mL/min, 60mL/min and 90mL/min) were studied. The best condition for spore production (3.3x10(10) CFU.g-¹dry matter) in column bioreactor was 80% of initial humidity and no aeration. In Erlenmeyer flasks and plastic bags the best sporulation production reached 1.7 up to 4.7x10(10) CFU.g-1dry matter with 88-93% of initial moisture. The aeration rate had no significant effect on the spore yield. The initial moisture had a significant effect depending on the bioreactor type. Sporulation kinetic's assay was carried out and it showed the possibility to reduce the time of spore formation in two days.