Production and characterization of surface-active compounds from Gordonia amicalis
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100019 |
Resumo: | Two methods were used to make crude preparations of surface-active compounds (SACs) produced by Gordonia amicalis grown on the medium containing 1% diesel oil. Using a 2:1 (v/v) solution of chloroform:methanol for extraction, Type I SACs were isolated and shown to produce oil in water (O/W) emulsions. Type II SACs were isolated by precipitation with ammonium sulfate and produced predominantly water in oil emulsions (W/O). The crude Type I and II preparations were able to produce a significant reduction in the surface tension of water; however, the crude Type II preparation had 10-25 fold higher emulsification activity than the Type I preparation. Both SAC preparations were analyzed by the TLC and each produced two distinct bands with Rf 0.44 and 0.62 and Rf 0.52 and 0.62, respectively. The partially purified SACs were characterized by the ESI(+)-MS, FT-IR and NMR. In each one of these fractions, a mixture of 10 oligomers was found consisting of a series of compounds, with masses from 502 to 899, differing in molecular mass by a repeating unit of 44 Daltons. The mass spectra of these compounds did not appear to match other known biosurfactants and could represent a novel class of these compounds. |
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Production and characterization of surface-active compounds from Gordonia amicalisBioemulsifierbioremediationbiosurfactantGordonia amicalisindustrial chemicalsmicrobial surfactant productionTwo methods were used to make crude preparations of surface-active compounds (SACs) produced by Gordonia amicalis grown on the medium containing 1% diesel oil. Using a 2:1 (v/v) solution of chloroform:methanol for extraction, Type I SACs were isolated and shown to produce oil in water (O/W) emulsions. Type II SACs were isolated by precipitation with ammonium sulfate and produced predominantly water in oil emulsions (W/O). The crude Type I and II preparations were able to produce a significant reduction in the surface tension of water; however, the crude Type II preparation had 10-25 fold higher emulsification activity than the Type I preparation. Both SAC preparations were analyzed by the TLC and each produced two distinct bands with Rf 0.44 and 0.62 and Rf 0.52 and 0.62, respectively. The partially purified SACs were characterized by the ESI(+)-MS, FT-IR and NMR. In each one of these fractions, a mixture of 10 oligomers was found consisting of a series of compounds, with masses from 502 to 899, differing in molecular mass by a repeating unit of 44 Daltons. The mass spectra of these compounds did not appear to match other known biosurfactants and could represent a novel class of these compounds.Instituto de Tecnologia do Paraná - Tecpar2014-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100019Brazilian Archives of Biology and Technology v.57 n.1 2014reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132014000100019info:eu-repo/semantics/openAccessJackisch-Matsuura,Ani BeatrizSantos,Leonardo SilvaEberlin,Marcos NogueiraFaria,Andréia Fonseca deMatsuura,TakeshiGrossman,Matthew JamesDurrant,Lucia Reginaeng2014-02-24T00:00:00Zoai:scielo:S1516-89132014000100019Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2014-02-24T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Production and characterization of surface-active compounds from Gordonia amicalis |
title |
Production and characterization of surface-active compounds from Gordonia amicalis |
spellingShingle |
Production and characterization of surface-active compounds from Gordonia amicalis Jackisch-Matsuura,Ani Beatriz Bioemulsifier bioremediation biosurfactant Gordonia amicalis industrial chemicals microbial surfactant production |
title_short |
Production and characterization of surface-active compounds from Gordonia amicalis |
title_full |
Production and characterization of surface-active compounds from Gordonia amicalis |
title_fullStr |
Production and characterization of surface-active compounds from Gordonia amicalis |
title_full_unstemmed |
Production and characterization of surface-active compounds from Gordonia amicalis |
title_sort |
Production and characterization of surface-active compounds from Gordonia amicalis |
author |
Jackisch-Matsuura,Ani Beatriz |
author_facet |
Jackisch-Matsuura,Ani Beatriz Santos,Leonardo Silva Eberlin,Marcos Nogueira Faria,Andréia Fonseca de Matsuura,Takeshi Grossman,Matthew James Durrant,Lucia Regina |
author_role |
author |
author2 |
Santos,Leonardo Silva Eberlin,Marcos Nogueira Faria,Andréia Fonseca de Matsuura,Takeshi Grossman,Matthew James Durrant,Lucia Regina |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Jackisch-Matsuura,Ani Beatriz Santos,Leonardo Silva Eberlin,Marcos Nogueira Faria,Andréia Fonseca de Matsuura,Takeshi Grossman,Matthew James Durrant,Lucia Regina |
dc.subject.por.fl_str_mv |
Bioemulsifier bioremediation biosurfactant Gordonia amicalis industrial chemicals microbial surfactant production |
topic |
Bioemulsifier bioremediation biosurfactant Gordonia amicalis industrial chemicals microbial surfactant production |
description |
Two methods were used to make crude preparations of surface-active compounds (SACs) produced by Gordonia amicalis grown on the medium containing 1% diesel oil. Using a 2:1 (v/v) solution of chloroform:methanol for extraction, Type I SACs were isolated and shown to produce oil in water (O/W) emulsions. Type II SACs were isolated by precipitation with ammonium sulfate and produced predominantly water in oil emulsions (W/O). The crude Type I and II preparations were able to produce a significant reduction in the surface tension of water; however, the crude Type II preparation had 10-25 fold higher emulsification activity than the Type I preparation. Both SAC preparations were analyzed by the TLC and each produced two distinct bands with Rf 0.44 and 0.62 and Rf 0.52 and 0.62, respectively. The partially purified SACs were characterized by the ESI(+)-MS, FT-IR and NMR. In each one of these fractions, a mixture of 10 oligomers was found consisting of a series of compounds, with masses from 502 to 899, differing in molecular mass by a repeating unit of 44 Daltons. The mass spectra of these compounds did not appear to match other known biosurfactants and could represent a novel class of these compounds. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100019 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100019 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132014000100019 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.57 n.1 2014 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318276073750528 |