Anticoagulant activity of a sulfated polysaccharide isolated from the green seaweed Caulerpa cupressoides

• Autor(a) principal: Rodrigues,José Ariévilo Gurgel
• Data de Publicação: 2011
• Outros Autores: Vanderlei,Edfranck de Sousa Oliveira, Bessa,Érika Façanha, Magalhães,Francisco de Araújo, Paula,Regina Célia Monteiro de, Lima,Vilma, Benevides,Norma Maria Barros
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Archives of Biology and Technology
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400007
• topic: marine algae; sulfated macromolecules; chemical analysis; APTT test

The aim of this study was to evaluate certain molecular characteristics of a sulfated polysaccharide (SPs) with anticoagulant properties, isolated from Caulerpa cupressoides (Chlorophyta). Crude SPs were extracted by proteolytic digestion (papain), followed by ion-exchange chromatography on a DEAE-cellulose column. The fractions obtained were analyzed for molecular mass, 0.5% agarose gel electrophoresis and chemical composition. The activated partial thromboplastin time (APTT) test was applied using normal human plasma and standard heparin (HEP) (193 IU mg-1). The yield was ~ 3%, and the chromatography procedure separated the material into three different SP fractions (F I, F II and F III), eluted at the concentrations of 0.50, 0.75 and 1.00 M of NaCl, respectively. Only fraction F II was active (24.62 IU mg-1), with high sulfate content (23.79%) and number of molecular mass peaks. Therefore, the APTT of a fraction isolated from C. cupressoides was less potent than HEP.