Adsorption of amyloglucosidase from Aspergillus niger NRRL 3122 using ion exchange resin

Detalhes bibliográficos
Autor(a) principal: Manera,Ana Paula
Data de Publicação: 2008
Outros Autores: Kamimura,Eliana Setsuko, Brites,Luciana Machado, Kalil,Susana Juliano
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500019
Resumo: Amyloglucosidase enzyme was produced by Aspergillus niger NRRL 3122 from solid-state fermentation, using deffated rice bran as substrate. The effects of process parameters (pH, temperature) in the equilibrium partition coefficient for the system amyloglucosidase - resin DEAE-cellulose were investigated, aiming at obtaining the optimum conditions for a subsequent purification process. The highest partition coefficients were obtained using 0.025M Tris-HCl buffer, pH 8.0 and 25ºC. The conditions that supplied the highest partition coefficient were specified, the isotherm that better described the amyloglucosidase process of adsorption obtained. It was observed that the adsorption could be well described by Langmuir equation and the values of Qm and Kd estimated at 133.0 U mL-1 and 15.4 U mL-1, respectively. From the adjustment of the kinetic curves using the fourth-order Runge-Kutta algorithm, the adsorption (k1) and desorption (k2) constants were obtained through optimization by the least square procedure, and the values calculated were 2.4x10-3 mL U-1 min-1 for k1 and 0.037 min-1 for k2 .
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spelling Adsorption of amyloglucosidase from Aspergillus niger NRRL 3122 using ion exchange resinamyloglucosidaseadsorption isothermkinetics adsorptionion exchange resinpurificationAmyloglucosidase enzyme was produced by Aspergillus niger NRRL 3122 from solid-state fermentation, using deffated rice bran as substrate. The effects of process parameters (pH, temperature) in the equilibrium partition coefficient for the system amyloglucosidase - resin DEAE-cellulose were investigated, aiming at obtaining the optimum conditions for a subsequent purification process. The highest partition coefficients were obtained using 0.025M Tris-HCl buffer, pH 8.0 and 25ºC. The conditions that supplied the highest partition coefficient were specified, the isotherm that better described the amyloglucosidase process of adsorption obtained. It was observed that the adsorption could be well described by Langmuir equation and the values of Qm and Kd estimated at 133.0 U mL-1 and 15.4 U mL-1, respectively. From the adjustment of the kinetic curves using the fourth-order Runge-Kutta algorithm, the adsorption (k1) and desorption (k2) constants were obtained through optimization by the least square procedure, and the values calculated were 2.4x10-3 mL U-1 min-1 for k1 and 0.037 min-1 for k2 .Instituto de Tecnologia do Paraná - Tecpar2008-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500019Brazilian Archives of Biology and Technology v.51 n.5 2008reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132008000500019info:eu-repo/semantics/openAccessManera,Ana PaulaKamimura,Eliana SetsukoBrites,Luciana MachadoKalil,Susana Julianoeng2008-10-31T00:00:00Zoai:scielo:S1516-89132008000500019Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2008-10-31T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Adsorption of amyloglucosidase from Aspergillus niger NRRL 3122 using ion exchange resin
title Adsorption of amyloglucosidase from Aspergillus niger NRRL 3122 using ion exchange resin
spellingShingle Adsorption of amyloglucosidase from Aspergillus niger NRRL 3122 using ion exchange resin
Manera,Ana Paula
amyloglucosidase
adsorption isotherm
kinetics adsorption
ion exchange resin
purification
title_short Adsorption of amyloglucosidase from Aspergillus niger NRRL 3122 using ion exchange resin
title_full Adsorption of amyloglucosidase from Aspergillus niger NRRL 3122 using ion exchange resin
title_fullStr Adsorption of amyloglucosidase from Aspergillus niger NRRL 3122 using ion exchange resin
title_full_unstemmed Adsorption of amyloglucosidase from Aspergillus niger NRRL 3122 using ion exchange resin
title_sort Adsorption of amyloglucosidase from Aspergillus niger NRRL 3122 using ion exchange resin
author Manera,Ana Paula
author_facet Manera,Ana Paula
Kamimura,Eliana Setsuko
Brites,Luciana Machado
Kalil,Susana Juliano
author_role author
author2 Kamimura,Eliana Setsuko
Brites,Luciana Machado
Kalil,Susana Juliano
author2_role author
author
author
dc.contributor.author.fl_str_mv Manera,Ana Paula
Kamimura,Eliana Setsuko
Brites,Luciana Machado
Kalil,Susana Juliano
dc.subject.por.fl_str_mv amyloglucosidase
adsorption isotherm
kinetics adsorption
ion exchange resin
purification
topic amyloglucosidase
adsorption isotherm
kinetics adsorption
ion exchange resin
purification
description Amyloglucosidase enzyme was produced by Aspergillus niger NRRL 3122 from solid-state fermentation, using deffated rice bran as substrate. The effects of process parameters (pH, temperature) in the equilibrium partition coefficient for the system amyloglucosidase - resin DEAE-cellulose were investigated, aiming at obtaining the optimum conditions for a subsequent purification process. The highest partition coefficients were obtained using 0.025M Tris-HCl buffer, pH 8.0 and 25ºC. The conditions that supplied the highest partition coefficient were specified, the isotherm that better described the amyloglucosidase process of adsorption obtained. It was observed that the adsorption could be well described by Langmuir equation and the values of Qm and Kd estimated at 133.0 U mL-1 and 15.4 U mL-1, respectively. From the adjustment of the kinetic curves using the fourth-order Runge-Kutta algorithm, the adsorption (k1) and desorption (k2) constants were obtained through optimization by the least square procedure, and the values calculated were 2.4x10-3 mL U-1 min-1 for k1 and 0.037 min-1 for k2 .
publishDate 2008
dc.date.none.fl_str_mv 2008-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500019
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500019
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132008000500019
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.51 n.5 2008
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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