Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substrates

Detalhes bibliográficos
Autor(a) principal: Papamichael,Emmanuel M
Data de Publicação: 1999
Outros Autores: Roustas,Michael K, Bieth,Joseph G
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300002
Resumo: In this study, the S1 - P1 and S3 - P3 interactions between papain and four synthetic peptide substrates were found as to be important. The values of Km were estimated as to be practically identical between these substrates; this latter is supporting the conclusions obtained by considering the estimated values of other kinetic parameters. Nevertheless, based on the estimated kcat and/or k cat/Km parameters of the used substrates, we concluded that an aromatic ring at the P3 position, and a positively charged side chain of the residue at the P1 position of the synthetic substrates were favored considerably their interaction with papain.
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spelling Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substratesPapain-SubstratesEnzyme-Substrate InteractionsIn this study, the S1 - P1 and S3 - P3 interactions between papain and four synthetic peptide substrates were found as to be important. The values of Km were estimated as to be practically identical between these substrates; this latter is supporting the conclusions obtained by considering the estimated values of other kinetic parameters. Nevertheless, based on the estimated kcat and/or k cat/Km parameters of the used substrates, we concluded that an aromatic ring at the P3 position, and a positively charged side chain of the residue at the P1 position of the synthetic substrates were favored considerably their interaction with papain.Instituto de Tecnologia do Paraná - Tecpar1999-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300002Brazilian Archives of Biology and Technology v.42 n.3 1999reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89131999000300002info:eu-repo/semantics/openAccessPapamichael,Emmanuel MRoustas,Michael KBieth,Joseph Geng2011-06-01T00:00:00Zoai:scielo:S1516-89131999000300002Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2011-06-01T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substrates
title Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substrates
spellingShingle Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substrates
Papamichael,Emmanuel M
Papain-Substrates
Enzyme-Substrate Interactions
title_short Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substrates
title_full Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substrates
title_fullStr Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substrates
title_full_unstemmed Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substrates
title_sort Detection of S1-P1 and S3-P3 interactions between papain and four synthetic substrates
author Papamichael,Emmanuel M
author_facet Papamichael,Emmanuel M
Roustas,Michael K
Bieth,Joseph G
author_role author
author2 Roustas,Michael K
Bieth,Joseph G
author2_role author
author
dc.contributor.author.fl_str_mv Papamichael,Emmanuel M
Roustas,Michael K
Bieth,Joseph G
dc.subject.por.fl_str_mv Papain-Substrates
Enzyme-Substrate Interactions
topic Papain-Substrates
Enzyme-Substrate Interactions
description In this study, the S1 - P1 and S3 - P3 interactions between papain and four synthetic peptide substrates were found as to be important. The values of Km were estimated as to be practically identical between these substrates; this latter is supporting the conclusions obtained by considering the estimated values of other kinetic parameters. Nevertheless, based on the estimated kcat and/or k cat/Km parameters of the used substrates, we concluded that an aromatic ring at the P3 position, and a positively charged side chain of the residue at the P1 position of the synthetic substrates were favored considerably their interaction with papain.
publishDate 1999
dc.date.none.fl_str_mv 1999-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300002
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300002
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89131999000300002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.42 n.3 1999
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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