Partition of glucose oxidase from Aspergillus niger in aqueous two-phase systems based on salt and polyethylene glycol

Detalhes bibliográficos
Autor(a) principal: Singh,Jagdish
Data de Publicação: 2010
Outros Autores: Verma,Neelam
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500007
Resumo: The aim of this work was to study the isolation of glucose oxidase (GOx) from Aspergillus niger in aqueous two phase system consisting of PEG 7500 (150g l-1), potassium phosphate (175 g l-1, K2HPO4 +KH2PO4) and glucose (10 gl-1), the enzyme was partitioned in polymer phase. By sequential extraction GOx (69.2%) was recovered in polymer phase by 11.8 fold purification, giving a yield of 129U mg protein-¹.
id TECPAR-1_4fa56737622c69fd6b9a531f52d04b53
oai_identifier_str oai:scielo:S1516-89132010000500007
network_acronym_str TECPAR-1
network_name_str Brazilian Archives of Biology and Technology
repository_id_str
spelling Partition of glucose oxidase from Aspergillus niger in aqueous two-phase systems based on salt and polyethylene glycolAspergillus nigerglucose oxidaseaqueous two phaseThe aim of this work was to study the isolation of glucose oxidase (GOx) from Aspergillus niger in aqueous two phase system consisting of PEG 7500 (150g l-1), potassium phosphate (175 g l-1, K2HPO4 +KH2PO4) and glucose (10 gl-1), the enzyme was partitioned in polymer phase. By sequential extraction GOx (69.2%) was recovered in polymer phase by 11.8 fold purification, giving a yield of 129U mg protein-¹.Instituto de Tecnologia do Paraná - Tecpar2010-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500007Brazilian Archives of Biology and Technology v.53 n.5 2010reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132010000500007info:eu-repo/semantics/openAccessSingh,JagdishVerma,Neelameng2010-11-03T00:00:00Zoai:scielo:S1516-89132010000500007Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2010-11-03T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Partition of glucose oxidase from Aspergillus niger in aqueous two-phase systems based on salt and polyethylene glycol
title Partition of glucose oxidase from Aspergillus niger in aqueous two-phase systems based on salt and polyethylene glycol
spellingShingle Partition of glucose oxidase from Aspergillus niger in aqueous two-phase systems based on salt and polyethylene glycol
Singh,Jagdish
Aspergillus niger
glucose oxidase
aqueous two phase
title_short Partition of glucose oxidase from Aspergillus niger in aqueous two-phase systems based on salt and polyethylene glycol
title_full Partition of glucose oxidase from Aspergillus niger in aqueous two-phase systems based on salt and polyethylene glycol
title_fullStr Partition of glucose oxidase from Aspergillus niger in aqueous two-phase systems based on salt and polyethylene glycol
title_full_unstemmed Partition of glucose oxidase from Aspergillus niger in aqueous two-phase systems based on salt and polyethylene glycol
title_sort Partition of glucose oxidase from Aspergillus niger in aqueous two-phase systems based on salt and polyethylene glycol
author Singh,Jagdish
author_facet Singh,Jagdish
Verma,Neelam
author_role author
author2 Verma,Neelam
author2_role author
dc.contributor.author.fl_str_mv Singh,Jagdish
Verma,Neelam
dc.subject.por.fl_str_mv Aspergillus niger
glucose oxidase
aqueous two phase
topic Aspergillus niger
glucose oxidase
aqueous two phase
description The aim of this work was to study the isolation of glucose oxidase (GOx) from Aspergillus niger in aqueous two phase system consisting of PEG 7500 (150g l-1), potassium phosphate (175 g l-1, K2HPO4 +KH2PO4) and glucose (10 gl-1), the enzyme was partitioned in polymer phase. By sequential extraction GOx (69.2%) was recovered in polymer phase by 11.8 fold purification, giving a yield of 129U mg protein-¹.
publishDate 2010
dc.date.none.fl_str_mv 2010-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132010000500007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.53 n.5 2010
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
_version_ 1750318274036367360

Registros relacionados