Ultrastructural and quantitative studies of hemocytes in the sugarcane borer, Diatraea saccharalis (Lepidoptera: Pyralidae)

• Autor(a) principal: Falleiros,Ângela Maria Ferreira
• Data de Publicação: 2003
• Outros Autores: Bombonato,Maria Terezinha Siqueira, Gregório,Elisa Aparecida
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Archives of Biology and Technology
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200021
• topic: Insect; Lepidoptera; Hemocytes; Ultrastructural Morphology; Differential Hemocyte Count; Morphometry

Six circulating hemocytes cell types from Diatraea saccharalis (Fabricius) (Lepidoptera: Pyralidae) larvae were identified by transmission and scanning electron microscope: prohemocytes (PR), plasmatocytes (PL) granulocytes (GR), spherulocytes (SP), oenocytoids (OE) and vermicytes (VE). The PR was the smallest cell type with a large nucleus, a cytoplasm with few organelles and a homogenous smooth surface. The PL was polymorphic and abundant, with a cytoplasm rich in organelles and a cellular surface with several cytoplasmic projections. The GR was abundant, showing two types of membrane-bounded granules (dense and structutered), glycogen, lipid droplets and a surface with philopodial projections. The SP was a large cell, with a cytoplasm full of intracytoplasmic spherules. The OE was the largest hemocyte type with a large and homogeneous cytoplasm and scarce organelles. The VE was discoid in shape and showed electron-dense granules.