Micropropagation of Endangered and Decorative Species Dianthus pinifolius Sibth. et Sm.

Detalhes bibliográficos
Autor(a) principal: Markovic,Marija
Data de Publicação: 2016
Outros Autores: Grbic,Mihailo, Djukic,Matilda
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100403
Resumo: The aim of this study was to establish an efficient protocol for the in vitro propagation of the endangered, endemic and decorative species Dianthus pinifolius Sibth. et Sm. The effects of different concentrations of 6-benzylaminopurine (BAP) and naphtalenacetic acid (NAA), and different explant types (single-node cuttings, terminal buds and shoot cuttings) on shoot multiplication were examined on Murashige and Skoog (MS) and half-strength MS media. The best results were obtained for shoot cuttings on the MS medium supplemented with 0.5 mg/L BAP and 0.1 mg/L NAA, achieving a maximum rate of regeneration (100%) and a total of 15.4 newly-developed shoots per explant. The highest rooting rate (96.7%) was obtained on MS medium containing 1 mg/L NAA, while the acclimatization of the microplants obtained to ex vitro conditions was successful (88.9%).
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spelling Micropropagation of Endangered and Decorative Species Dianthus pinifolius Sibth. et Sm.pineleaf pinkin vitro cultureBAPNAAmultiplicationThe aim of this study was to establish an efficient protocol for the in vitro propagation of the endangered, endemic and decorative species Dianthus pinifolius Sibth. et Sm. The effects of different concentrations of 6-benzylaminopurine (BAP) and naphtalenacetic acid (NAA), and different explant types (single-node cuttings, terminal buds and shoot cuttings) on shoot multiplication were examined on Murashige and Skoog (MS) and half-strength MS media. The best results were obtained for shoot cuttings on the MS medium supplemented with 0.5 mg/L BAP and 0.1 mg/L NAA, achieving a maximum rate of regeneration (100%) and a total of 15.4 newly-developed shoots per explant. The highest rooting rate (96.7%) was obtained on MS medium containing 1 mg/L NAA, while the acclimatization of the microplants obtained to ex vitro conditions was successful (88.9%).Instituto de Tecnologia do Paraná - Tecpar2016-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100403Brazilian Archives of Biology and Technology v.59 2016reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/1678-4324-2016150320info:eu-repo/semantics/openAccessMarkovic,MarijaGrbic,MihailoDjukic,Matildaeng2017-01-20T00:00:00Zoai:scielo:S1516-89132016000100403Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2017-01-20T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Micropropagation of Endangered and Decorative Species Dianthus pinifolius Sibth. et Sm.
title Micropropagation of Endangered and Decorative Species Dianthus pinifolius Sibth. et Sm.
spellingShingle Micropropagation of Endangered and Decorative Species Dianthus pinifolius Sibth. et Sm.
Markovic,Marija
pineleaf pink
in vitro culture
BAP
NAA
multiplication
title_short Micropropagation of Endangered and Decorative Species Dianthus pinifolius Sibth. et Sm.
title_full Micropropagation of Endangered and Decorative Species Dianthus pinifolius Sibth. et Sm.
title_fullStr Micropropagation of Endangered and Decorative Species Dianthus pinifolius Sibth. et Sm.
title_full_unstemmed Micropropagation of Endangered and Decorative Species Dianthus pinifolius Sibth. et Sm.
title_sort Micropropagation of Endangered and Decorative Species Dianthus pinifolius Sibth. et Sm.
author Markovic,Marija
author_facet Markovic,Marija
Grbic,Mihailo
Djukic,Matilda
author_role author
author2 Grbic,Mihailo
Djukic,Matilda
author2_role author
author
dc.contributor.author.fl_str_mv Markovic,Marija
Grbic,Mihailo
Djukic,Matilda
dc.subject.por.fl_str_mv pineleaf pink
in vitro culture
BAP
NAA
multiplication
topic pineleaf pink
in vitro culture
BAP
NAA
multiplication
description The aim of this study was to establish an efficient protocol for the in vitro propagation of the endangered, endemic and decorative species Dianthus pinifolius Sibth. et Sm. The effects of different concentrations of 6-benzylaminopurine (BAP) and naphtalenacetic acid (NAA), and different explant types (single-node cuttings, terminal buds and shoot cuttings) on shoot multiplication were examined on Murashige and Skoog (MS) and half-strength MS media. The best results were obtained for shoot cuttings on the MS medium supplemented with 0.5 mg/L BAP and 0.1 mg/L NAA, achieving a maximum rate of regeneration (100%) and a total of 15.4 newly-developed shoots per explant. The highest rooting rate (96.7%) was obtained on MS medium containing 1 mg/L NAA, while the acclimatization of the microplants obtained to ex vitro conditions was successful (88.9%).
publishDate 2016
dc.date.none.fl_str_mv 2016-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100403
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100403
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-4324-2016150320
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.59 2016
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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