Enhanced production of manganese peroxidase by Phanerochaete chrysosporium

Detalhes bibliográficos
Autor(a) principal: Urek,Raziye Ozturk
Data de Publicação: 2007
Outros Autores: Pazarlioglu,Nurdan Kasikara
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700001
Resumo: Production of manganese-dependent peroxidase (MnP) by the white-rot fungus Phanerochaete chrysosporium BKM-F-1767 (ATCC 24725) was monitored during growth in different media and growth conditions. The effect of some activators of MnP production, Mn2+, Tween 80, phenylmethylsulphonylfloride (PMSF), oxygen, temperature, pH, glycerol and nitrogen was studied. Supplementing the cultures with Tween 80 (0.05 %, v/v) and Mn2+ (174 µM) resulted a maximum MnP activity of 356 U/L which was approximately two times higher than that obtained in the control culture (without Tween 80). Decolourisation of Direct Blue 15 and Direct Green 6 (50 mg/L) was also achieved with MnP.
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spelling Enhanced production of manganese peroxidase by Phanerochaete chrysosporiumPhanerochaete chrysosporiummanganese dependent peroxidaseTween 80Mn2+phenylmethylsulphonylflorideProduction of manganese-dependent peroxidase (MnP) by the white-rot fungus Phanerochaete chrysosporium BKM-F-1767 (ATCC 24725) was monitored during growth in different media and growth conditions. The effect of some activators of MnP production, Mn2+, Tween 80, phenylmethylsulphonylfloride (PMSF), oxygen, temperature, pH, glycerol and nitrogen was studied. Supplementing the cultures with Tween 80 (0.05 %, v/v) and Mn2+ (174 µM) resulted a maximum MnP activity of 356 U/L which was approximately two times higher than that obtained in the control culture (without Tween 80). Decolourisation of Direct Blue 15 and Direct Green 6 (50 mg/L) was also achieved with MnP.Instituto de Tecnologia do Paraná - Tecpar2007-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700001Brazilian Archives of Biology and Technology v.50 n.6 2007reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132007000700001info:eu-repo/semantics/openAccessUrek,Raziye OzturkPazarlioglu,Nurdan Kasikaraeng2008-03-03T00:00:00Zoai:scielo:S1516-89132007000700001Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2008-03-03T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Enhanced production of manganese peroxidase by Phanerochaete chrysosporium
title Enhanced production of manganese peroxidase by Phanerochaete chrysosporium
spellingShingle Enhanced production of manganese peroxidase by Phanerochaete chrysosporium
Urek,Raziye Ozturk
Phanerochaete chrysosporium
manganese dependent peroxidase
Tween 80
Mn2+
phenylmethylsulphonylfloride
title_short Enhanced production of manganese peroxidase by Phanerochaete chrysosporium
title_full Enhanced production of manganese peroxidase by Phanerochaete chrysosporium
title_fullStr Enhanced production of manganese peroxidase by Phanerochaete chrysosporium
title_full_unstemmed Enhanced production of manganese peroxidase by Phanerochaete chrysosporium
title_sort Enhanced production of manganese peroxidase by Phanerochaete chrysosporium
author Urek,Raziye Ozturk
author_facet Urek,Raziye Ozturk
Pazarlioglu,Nurdan Kasikara
author_role author
author2 Pazarlioglu,Nurdan Kasikara
author2_role author
dc.contributor.author.fl_str_mv Urek,Raziye Ozturk
Pazarlioglu,Nurdan Kasikara
dc.subject.por.fl_str_mv Phanerochaete chrysosporium
manganese dependent peroxidase
Tween 80
Mn2+
phenylmethylsulphonylfloride
topic Phanerochaete chrysosporium
manganese dependent peroxidase
Tween 80
Mn2+
phenylmethylsulphonylfloride
description Production of manganese-dependent peroxidase (MnP) by the white-rot fungus Phanerochaete chrysosporium BKM-F-1767 (ATCC 24725) was monitored during growth in different media and growth conditions. The effect of some activators of MnP production, Mn2+, Tween 80, phenylmethylsulphonylfloride (PMSF), oxygen, temperature, pH, glycerol and nitrogen was studied. Supplementing the cultures with Tween 80 (0.05 %, v/v) and Mn2+ (174 µM) resulted a maximum MnP activity of 356 U/L which was approximately two times higher than that obtained in the control culture (without Tween 80). Decolourisation of Direct Blue 15 and Direct Green 6 (50 mg/L) was also achieved with MnP.
publishDate 2007
dc.date.none.fl_str_mv 2007-11-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700001
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132007000700001
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.50 n.6 2007
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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