Cloning and Overexpression of GmDREB2 Gene from a Vietnamese Drought-resistant Soybean Variety

Detalhes bibliográficos
Autor(a) principal: Tan,Dao Xuan
Data de Publicação: 2015
Outros Autores: Tuong,Ho Manh, Thuy,Vu Thi Thu, Son,Le Van, Mau,Chu Hoang
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500651
Resumo: ABSTRACTThis work studied the amplification, cloning and determination of the GmDREB2 gene from the soybean cultivar DT2008 and five Vietnamese local soybean cultivars (DT26, DT51, DVN5, CB, CBD) and designed the vector carrying the structure containing GmDREB2 gene from cultivar DT2008 (best drought tolerant). The coding region of GmDREB2 gene isolated from six soybean cultivars was 480 nucleotides in length, encoding 159 amino acids. The recombinant structure was designed as 35S- GmDREB2- c-myc and its expression was analysed in transgenic tobacco plants. Recombinant DREB2 protein was expressed in five transgenic tobacco lines with molecular weights close to 20 kDa. During the drought conditions, the proline accumulation of the transgenic tobacco lines was higher than on wild-type (WT) plants, with the rate from 211.17 to 332.44% after five days of drought stress, and from 262.79 to 466.04% after nine days of drought stress. The two lines, TG2 and TG12 had the highest increase rate. These results provided the basis to generate drought-tolerant soybean plants by GmDREB2 overexpression.
id TECPAR-1_5ce8ceac66e20775564c64fbc0a2bf92
oai_identifier_str oai:scielo:S1516-89132015000500651
network_acronym_str TECPAR-1
network_name_str Brazilian Archives of Biology and Technology
repository_id_str
spelling Cloning and Overexpression of GmDREB2 Gene from a Vietnamese Drought-resistant Soybean Varietydrought-resistantGmDREB2 genegene transfersoybeantranscription factorsABSTRACTThis work studied the amplification, cloning and determination of the GmDREB2 gene from the soybean cultivar DT2008 and five Vietnamese local soybean cultivars (DT26, DT51, DVN5, CB, CBD) and designed the vector carrying the structure containing GmDREB2 gene from cultivar DT2008 (best drought tolerant). The coding region of GmDREB2 gene isolated from six soybean cultivars was 480 nucleotides in length, encoding 159 amino acids. The recombinant structure was designed as 35S- GmDREB2- c-myc and its expression was analysed in transgenic tobacco plants. Recombinant DREB2 protein was expressed in five transgenic tobacco lines with molecular weights close to 20 kDa. During the drought conditions, the proline accumulation of the transgenic tobacco lines was higher than on wild-type (WT) plants, with the rate from 211.17 to 332.44% after five days of drought stress, and from 262.79 to 466.04% after nine days of drought stress. The two lines, TG2 and TG12 had the highest increase rate. These results provided the basis to generate drought-tolerant soybean plants by GmDREB2 overexpression.Instituto de Tecnologia do Paraná - Tecpar2015-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500651Brazilian Archives of Biology and Technology v.58 n.5 2015reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132015050170info:eu-repo/semantics/openAccessTan,Dao XuanTuong,Ho ManhThuy,Vu Thi ThuSon,Le VanMau,Chu Hoangeng2015-11-06T00:00:00Zoai:scielo:S1516-89132015000500651Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2015-11-06T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Cloning and Overexpression of GmDREB2 Gene from a Vietnamese Drought-resistant Soybean Variety
title Cloning and Overexpression of GmDREB2 Gene from a Vietnamese Drought-resistant Soybean Variety
spellingShingle Cloning and Overexpression of GmDREB2 Gene from a Vietnamese Drought-resistant Soybean Variety
Tan,Dao Xuan
drought-resistant
GmDREB2 gene
gene transfer
soybean
transcription factors
title_short Cloning and Overexpression of GmDREB2 Gene from a Vietnamese Drought-resistant Soybean Variety
title_full Cloning and Overexpression of GmDREB2 Gene from a Vietnamese Drought-resistant Soybean Variety
title_fullStr Cloning and Overexpression of GmDREB2 Gene from a Vietnamese Drought-resistant Soybean Variety
title_full_unstemmed Cloning and Overexpression of GmDREB2 Gene from a Vietnamese Drought-resistant Soybean Variety
title_sort Cloning and Overexpression of GmDREB2 Gene from a Vietnamese Drought-resistant Soybean Variety
author Tan,Dao Xuan
author_facet Tan,Dao Xuan
Tuong,Ho Manh
Thuy,Vu Thi Thu
Son,Le Van
Mau,Chu Hoang
author_role author
author2 Tuong,Ho Manh
Thuy,Vu Thi Thu
Son,Le Van
Mau,Chu Hoang
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Tan,Dao Xuan
Tuong,Ho Manh
Thuy,Vu Thi Thu
Son,Le Van
Mau,Chu Hoang
dc.subject.por.fl_str_mv drought-resistant
GmDREB2 gene
gene transfer
soybean
transcription factors
topic drought-resistant
GmDREB2 gene
gene transfer
soybean
transcription factors
description ABSTRACTThis work studied the amplification, cloning and determination of the GmDREB2 gene from the soybean cultivar DT2008 and five Vietnamese local soybean cultivars (DT26, DT51, DVN5, CB, CBD) and designed the vector carrying the structure containing GmDREB2 gene from cultivar DT2008 (best drought tolerant). The coding region of GmDREB2 gene isolated from six soybean cultivars was 480 nucleotides in length, encoding 159 amino acids. The recombinant structure was designed as 35S- GmDREB2- c-myc and its expression was analysed in transgenic tobacco plants. Recombinant DREB2 protein was expressed in five transgenic tobacco lines with molecular weights close to 20 kDa. During the drought conditions, the proline accumulation of the transgenic tobacco lines was higher than on wild-type (WT) plants, with the rate from 211.17 to 332.44% after five days of drought stress, and from 262.79 to 466.04% after nine days of drought stress. The two lines, TG2 and TG12 had the highest increase rate. These results provided the basis to generate drought-tolerant soybean plants by GmDREB2 overexpression.
publishDate 2015
dc.date.none.fl_str_mv 2015-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500651
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500651
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132015050170
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.58 n.5 2015
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
_version_ 1750318276950360064

Registros relacionados