Isolation of natural inhibitors of papain obtained from Carica papaya latex

Detalhes bibliográficos
Autor(a) principal: Monti,Rubens
Data de Publicação: 2004
Outros Autores: Contiero,Jonas, Goulart,Antonio José
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500010
Resumo: Studies were carried out to natural papain inhibitor from papaya latex. Fresh latex from green fruits of Carica papaya was collected and immediately transported in ice bath to the lab, from which three fractions with inhibitor effect of esterase papain activity were isolated by latex dialysis, Sephadex G-25 gel filtration and ionic exchange chromatography in SP-Sephadex C-25. The isolated fractions, identified as inhibitors I and II, showed a negative reaction with ninhydrin; however, the fraction identified as P-III showed positive reaction with ninhydrin. Kinetics data showed non-competitive inhibition (inhibitor I) and uncompetitive (inhibitors II and P-III).
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spelling Isolation of natural inhibitors of papain obtained from Carica papaya latexEsterase activitynatural papain inhibitorprotease inhibitorStudies were carried out to natural papain inhibitor from papaya latex. Fresh latex from green fruits of Carica papaya was collected and immediately transported in ice bath to the lab, from which three fractions with inhibitor effect of esterase papain activity were isolated by latex dialysis, Sephadex G-25 gel filtration and ionic exchange chromatography in SP-Sephadex C-25. The isolated fractions, identified as inhibitors I and II, showed a negative reaction with ninhydrin; however, the fraction identified as P-III showed positive reaction with ninhydrin. Kinetics data showed non-competitive inhibition (inhibitor I) and uncompetitive (inhibitors II and P-III).Instituto de Tecnologia do Paraná - Tecpar2004-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500010Brazilian Archives of Biology and Technology v.47 n.5 2004reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132004000500010info:eu-repo/semantics/openAccessMonti,RubensContiero,JonasGoulart,Antonio Joséeng2004-11-23T00:00:00Zoai:scielo:S1516-89132004000500010Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2004-11-23T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Isolation of natural inhibitors of papain obtained from Carica papaya latex
title Isolation of natural inhibitors of papain obtained from Carica papaya latex
spellingShingle Isolation of natural inhibitors of papain obtained from Carica papaya latex
Monti,Rubens
Esterase activity
natural papain inhibitor
protease inhibitor
title_short Isolation of natural inhibitors of papain obtained from Carica papaya latex
title_full Isolation of natural inhibitors of papain obtained from Carica papaya latex
title_fullStr Isolation of natural inhibitors of papain obtained from Carica papaya latex
title_full_unstemmed Isolation of natural inhibitors of papain obtained from Carica papaya latex
title_sort Isolation of natural inhibitors of papain obtained from Carica papaya latex
author Monti,Rubens
author_facet Monti,Rubens
Contiero,Jonas
Goulart,Antonio José
author_role author
author2 Contiero,Jonas
Goulart,Antonio José
author2_role author
author
dc.contributor.author.fl_str_mv Monti,Rubens
Contiero,Jonas
Goulart,Antonio José
dc.subject.por.fl_str_mv Esterase activity
natural papain inhibitor
protease inhibitor
topic Esterase activity
natural papain inhibitor
protease inhibitor
description Studies were carried out to natural papain inhibitor from papaya latex. Fresh latex from green fruits of Carica papaya was collected and immediately transported in ice bath to the lab, from which three fractions with inhibitor effect of esterase papain activity were isolated by latex dialysis, Sephadex G-25 gel filtration and ionic exchange chromatography in SP-Sephadex C-25. The isolated fractions, identified as inhibitors I and II, showed a negative reaction with ninhydrin; however, the fraction identified as P-III showed positive reaction with ninhydrin. Kinetics data showed non-competitive inhibition (inhibitor I) and uncompetitive (inhibitors II and P-III).
publishDate 2004
dc.date.none.fl_str_mv 2004-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500010
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132004000500010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.47 n.5 2004
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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