Isolation and culture of soybean (Glycine max L. Merrill) microspores and pollen grains

Detalhes bibliográficos
Autor(a) principal: Rodrigues,Lia Rosane
Data de Publicação: 2006
Outros Autores: Forte,Bianca de Camargo, Bodanese-Zanettini,Maria Helena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500002
Resumo: In the last three decades, research on soybean microspore embryogenesis was restricted to anther culture, which presents limitations such as the small number of responsive microspores and the high embryogenic potential of sporophytic tissues. Therefore, a sequence of studies was performed to establish appropriate conditions for the isolation and culture of soybean microspores and pollen grains as an alternative to anther culture. First, a pollen and microspore isolation technique was developed using floral buds from four soybean cultivars (Bragg, IAS 5, MG/BR-46 Conquista and BRSMT Uirapuru). This technique allowed the establishment of cultures with satisfactory density and characteristics. Subsequently, different culture conditions were tested. Although B5 and MS media have been currently recommended for soybean anther culture, the best result was obtained in PTA-15 modified medium, with the formation of enlarged microspores and 0.4% of multicellular pollen grains in the cultivar BRSMT Uirapuru.
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spelling Isolation and culture of soybean (Glycine max L. Merrill) microspores and pollen grainsAndrogenesisGlycine maxmicrospore culturemicrospore embryogenesismicrospore isolationsoybeanIn the last three decades, research on soybean microspore embryogenesis was restricted to anther culture, which presents limitations such as the small number of responsive microspores and the high embryogenic potential of sporophytic tissues. Therefore, a sequence of studies was performed to establish appropriate conditions for the isolation and culture of soybean microspores and pollen grains as an alternative to anther culture. First, a pollen and microspore isolation technique was developed using floral buds from four soybean cultivars (Bragg, IAS 5, MG/BR-46 Conquista and BRSMT Uirapuru). This technique allowed the establishment of cultures with satisfactory density and characteristics. Subsequently, different culture conditions were tested. Although B5 and MS media have been currently recommended for soybean anther culture, the best result was obtained in PTA-15 modified medium, with the formation of enlarged microspores and 0.4% of multicellular pollen grains in the cultivar BRSMT Uirapuru.Instituto de Tecnologia do Paraná - Tecpar2006-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500002Brazilian Archives of Biology and Technology v.49 n.4 2006reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132006000500002info:eu-repo/semantics/openAccessRodrigues,Lia RosaneForte,Bianca de CamargoBodanese-Zanettini,Maria Helenaeng2006-08-30T00:00:00Zoai:scielo:S1516-89132006000500002Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2006-08-30T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Isolation and culture of soybean (Glycine max L. Merrill) microspores and pollen grains
title Isolation and culture of soybean (Glycine max L. Merrill) microspores and pollen grains
spellingShingle Isolation and culture of soybean (Glycine max L. Merrill) microspores and pollen grains
Rodrigues,Lia Rosane
Androgenesis
Glycine max
microspore culture
microspore embryogenesis
microspore isolation
soybean
title_short Isolation and culture of soybean (Glycine max L. Merrill) microspores and pollen grains
title_full Isolation and culture of soybean (Glycine max L. Merrill) microspores and pollen grains
title_fullStr Isolation and culture of soybean (Glycine max L. Merrill) microspores and pollen grains
title_full_unstemmed Isolation and culture of soybean (Glycine max L. Merrill) microspores and pollen grains
title_sort Isolation and culture of soybean (Glycine max L. Merrill) microspores and pollen grains
author Rodrigues,Lia Rosane
author_facet Rodrigues,Lia Rosane
Forte,Bianca de Camargo
Bodanese-Zanettini,Maria Helena
author_role author
author2 Forte,Bianca de Camargo
Bodanese-Zanettini,Maria Helena
author2_role author
author
dc.contributor.author.fl_str_mv Rodrigues,Lia Rosane
Forte,Bianca de Camargo
Bodanese-Zanettini,Maria Helena
dc.subject.por.fl_str_mv Androgenesis
Glycine max
microspore culture
microspore embryogenesis
microspore isolation
soybean
topic Androgenesis
Glycine max
microspore culture
microspore embryogenesis
microspore isolation
soybean
description In the last three decades, research on soybean microspore embryogenesis was restricted to anther culture, which presents limitations such as the small number of responsive microspores and the high embryogenic potential of sporophytic tissues. Therefore, a sequence of studies was performed to establish appropriate conditions for the isolation and culture of soybean microspores and pollen grains as an alternative to anther culture. First, a pollen and microspore isolation technique was developed using floral buds from four soybean cultivars (Bragg, IAS 5, MG/BR-46 Conquista and BRSMT Uirapuru). This technique allowed the establishment of cultures with satisfactory density and characteristics. Subsequently, different culture conditions were tested. Although B5 and MS media have been currently recommended for soybean anther culture, the best result was obtained in PTA-15 modified medium, with the formation of enlarged microspores and 0.4% of multicellular pollen grains in the cultivar BRSMT Uirapuru.
publishDate 2006
dc.date.none.fl_str_mv 2006-07-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500002
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500002
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132006000500002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.49 n.4 2006
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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