Isolation and culture of soybean (Glycine max L. Merrill) microspores and pollen grains
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500002 |
Resumo: | In the last three decades, research on soybean microspore embryogenesis was restricted to anther culture, which presents limitations such as the small number of responsive microspores and the high embryogenic potential of sporophytic tissues. Therefore, a sequence of studies was performed to establish appropriate conditions for the isolation and culture of soybean microspores and pollen grains as an alternative to anther culture. First, a pollen and microspore isolation technique was developed using floral buds from four soybean cultivars (Bragg, IAS 5, MG/BR-46 Conquista and BRSMT Uirapuru). This technique allowed the establishment of cultures with satisfactory density and characteristics. Subsequently, different culture conditions were tested. Although B5 and MS media have been currently recommended for soybean anther culture, the best result was obtained in PTA-15 modified medium, with the formation of enlarged microspores and 0.4% of multicellular pollen grains in the cultivar BRSMT Uirapuru. |
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Isolation and culture of soybean (Glycine max L. Merrill) microspores and pollen grainsAndrogenesisGlycine maxmicrospore culturemicrospore embryogenesismicrospore isolationsoybeanIn the last three decades, research on soybean microspore embryogenesis was restricted to anther culture, which presents limitations such as the small number of responsive microspores and the high embryogenic potential of sporophytic tissues. Therefore, a sequence of studies was performed to establish appropriate conditions for the isolation and culture of soybean microspores and pollen grains as an alternative to anther culture. First, a pollen and microspore isolation technique was developed using floral buds from four soybean cultivars (Bragg, IAS 5, MG/BR-46 Conquista and BRSMT Uirapuru). This technique allowed the establishment of cultures with satisfactory density and characteristics. Subsequently, different culture conditions were tested. Although B5 and MS media have been currently recommended for soybean anther culture, the best result was obtained in PTA-15 modified medium, with the formation of enlarged microspores and 0.4% of multicellular pollen grains in the cultivar BRSMT Uirapuru.Instituto de Tecnologia do Paraná - Tecpar2006-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500002Brazilian Archives of Biology and Technology v.49 n.4 2006reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132006000500002info:eu-repo/semantics/openAccessRodrigues,Lia RosaneForte,Bianca de CamargoBodanese-Zanettini,Maria Helenaeng2006-08-30T00:00:00Zoai:scielo:S1516-89132006000500002Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2006-08-30T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Isolation and culture of soybean (Glycine max L. Merrill) microspores and pollen grains |
title |
Isolation and culture of soybean (Glycine max L. Merrill) microspores and pollen grains |
spellingShingle |
Isolation and culture of soybean (Glycine max L. Merrill) microspores and pollen grains Rodrigues,Lia Rosane Androgenesis Glycine max microspore culture microspore embryogenesis microspore isolation soybean |
title_short |
Isolation and culture of soybean (Glycine max L. Merrill) microspores and pollen grains |
title_full |
Isolation and culture of soybean (Glycine max L. Merrill) microspores and pollen grains |
title_fullStr |
Isolation and culture of soybean (Glycine max L. Merrill) microspores and pollen grains |
title_full_unstemmed |
Isolation and culture of soybean (Glycine max L. Merrill) microspores and pollen grains |
title_sort |
Isolation and culture of soybean (Glycine max L. Merrill) microspores and pollen grains |
author |
Rodrigues,Lia Rosane |
author_facet |
Rodrigues,Lia Rosane Forte,Bianca de Camargo Bodanese-Zanettini,Maria Helena |
author_role |
author |
author2 |
Forte,Bianca de Camargo Bodanese-Zanettini,Maria Helena |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Rodrigues,Lia Rosane Forte,Bianca de Camargo Bodanese-Zanettini,Maria Helena |
dc.subject.por.fl_str_mv |
Androgenesis Glycine max microspore culture microspore embryogenesis microspore isolation soybean |
topic |
Androgenesis Glycine max microspore culture microspore embryogenesis microspore isolation soybean |
description |
In the last three decades, research on soybean microspore embryogenesis was restricted to anther culture, which presents limitations such as the small number of responsive microspores and the high embryogenic potential of sporophytic tissues. Therefore, a sequence of studies was performed to establish appropriate conditions for the isolation and culture of soybean microspores and pollen grains as an alternative to anther culture. First, a pollen and microspore isolation technique was developed using floral buds from four soybean cultivars (Bragg, IAS 5, MG/BR-46 Conquista and BRSMT Uirapuru). This technique allowed the establishment of cultures with satisfactory density and characteristics. Subsequently, different culture conditions were tested. Although B5 and MS media have been currently recommended for soybean anther culture, the best result was obtained in PTA-15 modified medium, with the formation of enlarged microspores and 0.4% of multicellular pollen grains in the cultivar BRSMT Uirapuru. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-07-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500002 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132006000500002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.49 n.4 2006 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318271096160256 |