Extra-cellular isoamylase production by Rhizopus oryzae in solid-state fermentation of agro wastes
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500003 |
Resumo: | Extra-cellular isoamylase was produced by Rhizopus oryzae PR7 in solid-state fermentations of various agro wastes, among which millet, oat, tapioca, and arum (Colocasia esculenta) showed promising results. The highest amount of enzyme production was obtained after 72 h of growth at 28°C. The optimum pH for enzyme production was - 8.0. Among the various additives tested, enzyme production increased with ions such as Ca2+, Mg2+ and also with cysteine, GSH, and DTT. The enzyme synthesis was reduced in the presence of thiol inhibitors like Cu2+ and pCMB. The surfactants like Tween-40, Tween-80 and Triton X-100 helped in enhancing the enzyme activity. The production could be further increased by using the combinations of substrates. The ability to produce high amount of isoamylase within a relatively very short period and the capability of degrading wastes could make the strain suitable for commercial production of the enzyme. |
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Extra-cellular isoamylase production by Rhizopus oryzae in solid-state fermentation of agro wastesIsoamylaseRhizopus oryzaeSSFwaste utilizationExtra-cellular isoamylase was produced by Rhizopus oryzae PR7 in solid-state fermentations of various agro wastes, among which millet, oat, tapioca, and arum (Colocasia esculenta) showed promising results. The highest amount of enzyme production was obtained after 72 h of growth at 28°C. The optimum pH for enzyme production was - 8.0. Among the various additives tested, enzyme production increased with ions such as Ca2+, Mg2+ and also with cysteine, GSH, and DTT. The enzyme synthesis was reduced in the presence of thiol inhibitors like Cu2+ and pCMB. The surfactants like Tween-40, Tween-80 and Triton X-100 helped in enhancing the enzyme activity. The production could be further increased by using the combinations of substrates. The ability to produce high amount of isoamylase within a relatively very short period and the capability of degrading wastes could make the strain suitable for commercial production of the enzyme.Instituto de Tecnologia do Paraná - Tecpar2011-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500003Brazilian Archives of Biology and Technology v.54 n.5 2011reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132011000500003info:eu-repo/semantics/openAccessGhosh,BarnitaRay,Rina Ranieng2011-11-01T00:00:00Zoai:scielo:S1516-89132011000500003Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2011-11-01T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Extra-cellular isoamylase production by Rhizopus oryzae in solid-state fermentation of agro wastes |
title |
Extra-cellular isoamylase production by Rhizopus oryzae in solid-state fermentation of agro wastes |
spellingShingle |
Extra-cellular isoamylase production by Rhizopus oryzae in solid-state fermentation of agro wastes Ghosh,Barnita Isoamylase Rhizopus oryzae SSF waste utilization |
title_short |
Extra-cellular isoamylase production by Rhizopus oryzae in solid-state fermentation of agro wastes |
title_full |
Extra-cellular isoamylase production by Rhizopus oryzae in solid-state fermentation of agro wastes |
title_fullStr |
Extra-cellular isoamylase production by Rhizopus oryzae in solid-state fermentation of agro wastes |
title_full_unstemmed |
Extra-cellular isoamylase production by Rhizopus oryzae in solid-state fermentation of agro wastes |
title_sort |
Extra-cellular isoamylase production by Rhizopus oryzae in solid-state fermentation of agro wastes |
author |
Ghosh,Barnita |
author_facet |
Ghosh,Barnita Ray,Rina Rani |
author_role |
author |
author2 |
Ray,Rina Rani |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Ghosh,Barnita Ray,Rina Rani |
dc.subject.por.fl_str_mv |
Isoamylase Rhizopus oryzae SSF waste utilization |
topic |
Isoamylase Rhizopus oryzae SSF waste utilization |
description |
Extra-cellular isoamylase was produced by Rhizopus oryzae PR7 in solid-state fermentations of various agro wastes, among which millet, oat, tapioca, and arum (Colocasia esculenta) showed promising results. The highest amount of enzyme production was obtained after 72 h of growth at 28°C. The optimum pH for enzyme production was - 8.0. Among the various additives tested, enzyme production increased with ions such as Ca2+, Mg2+ and also with cysteine, GSH, and DTT. The enzyme synthesis was reduced in the presence of thiol inhibitors like Cu2+ and pCMB. The surfactants like Tween-40, Tween-80 and Triton X-100 helped in enhancing the enzyme activity. The production could be further increased by using the combinations of substrates. The ability to produce high amount of isoamylase within a relatively very short period and the capability of degrading wastes could make the strain suitable for commercial production of the enzyme. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500003 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132011000500003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.54 n.5 2011 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318274817556480 |