Analysis of seven STR human loci for paternity testing by microchip electrophoresis

Detalhes bibliográficos
Autor(a) principal: Fraige,Karina
Data de Publicação: 2013
Outros Autores: Travensolo,Regiane Fátima, Carrilho,Emanuel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200006
Resumo: The aim of this work was to evaluate two paternity cases by microchip electrophoresis and the validation of the methodology by comparison of the results with those obtained in a commercial genetic analyzer. It was observed that when working with tetranucleotide regions, in which the minimal difference between the alleles was only four base pairs, the commercial microchip system did not present the resolution and repeatability needed. Nevertheless, the relative standard deviation was between 0 and 1.2% and the fragments detected were within the expected size ranges as described in the literature.
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spelling Analysis of seven STR human loci for paternity testing by microchip electrophoresisBioanalyzer 2100paternity testingpolymerase chain reactionshort tandem repeatsThe aim of this work was to evaluate two paternity cases by microchip electrophoresis and the validation of the methodology by comparison of the results with those obtained in a commercial genetic analyzer. It was observed that when working with tetranucleotide regions, in which the minimal difference between the alleles was only four base pairs, the commercial microchip system did not present the resolution and repeatability needed. Nevertheless, the relative standard deviation was between 0 and 1.2% and the fragments detected were within the expected size ranges as described in the literature.Instituto de Tecnologia do Paraná - Tecpar2013-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200006Brazilian Archives of Biology and Technology v.56 n.2 2013reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132013000200006info:eu-repo/semantics/openAccessFraige,KarinaTravensolo,Regiane FátimaCarrilho,Emanueleng2013-05-24T00:00:00Zoai:scielo:S1516-89132013000200006Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2013-05-24T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Analysis of seven STR human loci for paternity testing by microchip electrophoresis
title Analysis of seven STR human loci for paternity testing by microchip electrophoresis
spellingShingle Analysis of seven STR human loci for paternity testing by microchip electrophoresis
Fraige,Karina
Bioanalyzer 2100
paternity testing
polymerase chain reaction
short tandem repeats
title_short Analysis of seven STR human loci for paternity testing by microchip electrophoresis
title_full Analysis of seven STR human loci for paternity testing by microchip electrophoresis
title_fullStr Analysis of seven STR human loci for paternity testing by microchip electrophoresis
title_full_unstemmed Analysis of seven STR human loci for paternity testing by microchip electrophoresis
title_sort Analysis of seven STR human loci for paternity testing by microchip electrophoresis
author Fraige,Karina
author_facet Fraige,Karina
Travensolo,Regiane Fátima
Carrilho,Emanuel
author_role author
author2 Travensolo,Regiane Fátima
Carrilho,Emanuel
author2_role author
author
dc.contributor.author.fl_str_mv Fraige,Karina
Travensolo,Regiane Fátima
Carrilho,Emanuel
dc.subject.por.fl_str_mv Bioanalyzer 2100
paternity testing
polymerase chain reaction
short tandem repeats
topic Bioanalyzer 2100
paternity testing
polymerase chain reaction
short tandem repeats
description The aim of this work was to evaluate two paternity cases by microchip electrophoresis and the validation of the methodology by comparison of the results with those obtained in a commercial genetic analyzer. It was observed that when working with tetranucleotide regions, in which the minimal difference between the alleles was only four base pairs, the commercial microchip system did not present the resolution and repeatability needed. Nevertheless, the relative standard deviation was between 0 and 1.2% and the fragments detected were within the expected size ranges as described in the literature.
publishDate 2013
dc.date.none.fl_str_mv 2013-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200006
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132013000200006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.56 n.2 2013
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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