Somatic embryogenesis and plant regeneration in Wedelia calendulacea Less. an endangered medicinal plant
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300013 |
Resumo: | In this work, plant regeneration via somatic embryogenesis was achieved from leaf and internode derived callus of Wedelia calendulacea, an endangered medicinal plant. Primary callus was induced by culturing leaf disc and internode explant on Murashige and Skoog medium supplemented with 2.0 mg L-1 of 2,4-D under light condition. Transfer of embryogenic callus on a reduced concentration of 2,4-D facilitated somatic embryo development while calluses remained unorganized at the same 2,4-D level. A histological analysis confirmed somatic embryo by revealing the presence of a closed vascular system in the developing embryos and lack of a vascularconnection with surrounding callus tissues. Somatic embryos germinated into plantlets upon transfer on MS medium containing 1.0 mg L-1 BAP plus 0.5 mg L-1 GA3. Plantlets were acclimatized successfully and survived under soil condition. This is the first on somatic embryogenesis of W.calendulacea. This result could facilitate genetic transformation of this important medicinal plant. |
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Somatic embryogenesis and plant regeneration in Wedelia calendulacea Less. an endangered medicinal plantIn vitroMedicinal plantMicropropagationSomatic embryoThreatenedWedelia calendulaceaIn this work, plant regeneration via somatic embryogenesis was achieved from leaf and internode derived callus of Wedelia calendulacea, an endangered medicinal plant. Primary callus was induced by culturing leaf disc and internode explant on Murashige and Skoog medium supplemented with 2.0 mg L-1 of 2,4-D under light condition. Transfer of embryogenic callus on a reduced concentration of 2,4-D facilitated somatic embryo development while calluses remained unorganized at the same 2,4-D level. A histological analysis confirmed somatic embryo by revealing the presence of a closed vascular system in the developing embryos and lack of a vascularconnection with surrounding callus tissues. Somatic embryos germinated into plantlets upon transfer on MS medium containing 1.0 mg L-1 BAP plus 0.5 mg L-1 GA3. Plantlets were acclimatized successfully and survived under soil condition. This is the first on somatic embryogenesis of W.calendulacea. This result could facilitate genetic transformation of this important medicinal plant.Instituto de Tecnologia do Paraná - Tecpar2014-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300013Brazilian Archives of Biology and Technology v.57 n.3 2014reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-8913201401840info:eu-repo/semantics/openAccessSharmin,Shamima AkhtarAlam,Md. JahangirSheikh,Md. Mominul IslamSarker,Kanak KantiKhalekuzzaman,MuhammadHaque,Md. AnwarulAlam,Mohammad FirozAlam,Iftekhareng2014-05-30T00:00:00Zoai:scielo:S1516-89132014000300013Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2014-05-30T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Somatic embryogenesis and plant regeneration in Wedelia calendulacea Less. an endangered medicinal plant |
title |
Somatic embryogenesis and plant regeneration in Wedelia calendulacea Less. an endangered medicinal plant |
spellingShingle |
Somatic embryogenesis and plant regeneration in Wedelia calendulacea Less. an endangered medicinal plant Sharmin,Shamima Akhtar In vitro Medicinal plant Micropropagation Somatic embryo Threatened Wedelia calendulacea |
title_short |
Somatic embryogenesis and plant regeneration in Wedelia calendulacea Less. an endangered medicinal plant |
title_full |
Somatic embryogenesis and plant regeneration in Wedelia calendulacea Less. an endangered medicinal plant |
title_fullStr |
Somatic embryogenesis and plant regeneration in Wedelia calendulacea Less. an endangered medicinal plant |
title_full_unstemmed |
Somatic embryogenesis and plant regeneration in Wedelia calendulacea Less. an endangered medicinal plant |
title_sort |
Somatic embryogenesis and plant regeneration in Wedelia calendulacea Less. an endangered medicinal plant |
author |
Sharmin,Shamima Akhtar |
author_facet |
Sharmin,Shamima Akhtar Alam,Md. Jahangir Sheikh,Md. Mominul Islam Sarker,Kanak Kanti Khalekuzzaman,Muhammad Haque,Md. Anwarul Alam,Mohammad Firoz Alam,Iftekhar |
author_role |
author |
author2 |
Alam,Md. Jahangir Sheikh,Md. Mominul Islam Sarker,Kanak Kanti Khalekuzzaman,Muhammad Haque,Md. Anwarul Alam,Mohammad Firoz Alam,Iftekhar |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Sharmin,Shamima Akhtar Alam,Md. Jahangir Sheikh,Md. Mominul Islam Sarker,Kanak Kanti Khalekuzzaman,Muhammad Haque,Md. Anwarul Alam,Mohammad Firoz Alam,Iftekhar |
dc.subject.por.fl_str_mv |
In vitro Medicinal plant Micropropagation Somatic embryo Threatened Wedelia calendulacea |
topic |
In vitro Medicinal plant Micropropagation Somatic embryo Threatened Wedelia calendulacea |
description |
In this work, plant regeneration via somatic embryogenesis was achieved from leaf and internode derived callus of Wedelia calendulacea, an endangered medicinal plant. Primary callus was induced by culturing leaf disc and internode explant on Murashige and Skoog medium supplemented with 2.0 mg L-1 of 2,4-D under light condition. Transfer of embryogenic callus on a reduced concentration of 2,4-D facilitated somatic embryo development while calluses remained unorganized at the same 2,4-D level. A histological analysis confirmed somatic embryo by revealing the presence of a closed vascular system in the developing embryos and lack of a vascularconnection with surrounding callus tissues. Somatic embryos germinated into plantlets upon transfer on MS medium containing 1.0 mg L-1 BAP plus 0.5 mg L-1 GA3. Plantlets were acclimatized successfully and survived under soil condition. This is the first on somatic embryogenesis of W.calendulacea. This result could facilitate genetic transformation of this important medicinal plant. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300013 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300013 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-8913201401840 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.57 n.3 2014 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318276129325056 |