Performance evaluation of CHO-K1 cell in culture medium supplemented with hemolymph

• Autor(a) principal: Raffoul,Tássia
• Data de Publicação: 2005
• Outros Autores: Swiech,Kamilla, Arantes,Mabel Karina, Sousa,Álvaro Paiva Braga de, Mendonça,Ronaldo Zucatelli, Pereira,Carlos Augusto, Suazo,Cláudio Alberto Torres
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Archives of Biology and Technology
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400011
• topic: Animal cell; CHO-K1; microcarrier; growth; attachment; hemolymph

The aim of this work was to evaluate the potential of hemolymph utilization as a culture medium supplement to cultivate the animal cell CHO-K1. For this purpose 1% v/v of hemolymph was added to DMEM medium containing 10% v/v of FBS and 1 or 4.5 g/L of glucose. The culture was grown in spinner flasks incubated in a 10% v/v CO2 environment, at 37ºC, with the Cytodex 1 microcarrier. Comparing the results obtained from the culture with hemolymph against those without hemolymph, a positive influence of the hemolymph was observed, as the experiment with hemolymph presented a 52% higher cell concentration and a higher productivity of up to 40%.