Development of a Low Cost Bioprocess for Endotoxin Production by Bacillus thuringiensis var israelensis Intended for Biological Control of Aedes aegypti

• Autor(a) principal: Soccol,Carlos Ricardo
• Data de Publicação: 2009
• Outros Autores: Pollom,Teresinha E. V., Fendrich,Ricardo Cancio, Prochmann,Fernando Alberto, Mohan,Radijiskumar, Blaskowski,Marita Maciel Moreira, Melo,André Luiz de Almeida, Carvalho,Cláudio Jose Barros de, Soccol,Vanete Thomaz
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Archives of Biology and Technology
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700017
• topic: Biopesticide; BTI; Dengue Fever Disease; Soybean meal; Sugarcane molasses

Aedes aegypti is the vector of Dengue disease, responsible for 20,000 deaths/year worldwide. Bacillus thuringiensis var israelensis - Bti releases selective and effective toxins (crystal proteins) against A. aegypti larvae. We present a low cost bioprocess for toxin production, accomplished by a selected Brazilian strain Bti (BR-LPB01) and employment of low cost substrates. Soybean meal and sugarcane molasses lead to high toxic effectiveness after 2L bioreactor fermentation (LD50=26ng/mL), near to the reference strain IPS82 (LD50=17.3 ng/mL). The pH ranged between 5.8 and 7.0 during the exponential growth period and between 7.0 and 8.4 during the stationary phase, with low activity. Thus, control of foam and pH 7.0 were started and proved to be crucial for high activity. It was verified that the fermentation could be discontinued after 20 hours, when the highest activity was present.