In Vitro Organogenesis from Root Explants of Passiflora miniata Mast., an Amazonian Species with Ornamental Potential

• Autor(a) principal: Carvalho,Paula Pinheiro de
• Data de Publicação: 2019
• Outros Autores: Antoniazzi,Camila Aparecida, Faria,Rodrigo Brito de, Carvalho,Ilio Fealho de, Rocha,Diego Ismael, Silva,Maurecilne Lemes da
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Archives of Biology and Technology
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100406
• topic: Morphogenesis; Ornamental passion fruit; Plant regeneration; Root explant; Shoot organogenesis; Thidiazuron

ABSTRACT The present study reports a shoot organogenesis-based system for in vitro regeneration of Passiflora miniata, an Amazonia passion fruit species. Root segments were cultured in Murashige and Skoog (MS) medium supplemented with different concentrations (range 2-9 µM) of 6-benzyladenine (BA); thidiazuron (TDZ) or kinetin (KIN). Plant growth regulators were not added to the control treatment. Root explants have showed a high regenerative potential. After 30 days of in vitro culture, the root explants showed several shoots formed direct and indirectly. TDZ provided the best response in the differentiation adventitious shoots, mainly in the presence of 6.8 µM. The cytokinins BA and KIN responded producing a reduced number of shoots. After 120 days, rooted regenerated plants were transferred to a greenhouse for acclimatization. This regeneration system opens new perspectives for micropropagation and conservation of this wild Amazonic passion fruit species.