Development of an enzyme immunoassay for poliovirus antigens

Detalhes bibliográficos
Autor(a) principal: Hashimoto,Newton
Data de Publicação: 2007
Outros Autores: Benati,Fabrício José, Lauretti,Flávio, Linhares,Rosa Elisa Carvalho, Nozawa,Carlos Mitihiko
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100005
Resumo: An indirect solid-phase enzyme immunoassay (EIA) was developed for the detection of poliovirus antigen. Virus antigen was obtained in LLC-MK2 cell cultures and used to prepare antibodies in rabbit and guinea pig. Antibodies were evaluated by double immunodiffusion and neutralization test. Optimal concentrations of guinea pig and rabbit immunoglobulins were determined by checkerboard titration. Microtitre plates were coated with 15.0 µg/ml guinea pig anti-polio immunoglobulin and rabbit anti-polio immunoglobulin at the concentration of 7.94 µg/ml was used as detecting antibody. The standard curve with eight different antigen concentrations in eight replicates resulted in a coefficient of variation (CV) between 2.1% to 7.8%. The dose-response relationship was determined by simple linear regression with a coefficient of correlation (R²) equal to 96.4%. The assay detected a minimum of 2.3 µg/ml poliovirus antigen.
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spelling Development of an enzyme immunoassay for poliovirus antigensEnzyme immunoassayPoliovirus antigenAn indirect solid-phase enzyme immunoassay (EIA) was developed for the detection of poliovirus antigen. Virus antigen was obtained in LLC-MK2 cell cultures and used to prepare antibodies in rabbit and guinea pig. Antibodies were evaluated by double immunodiffusion and neutralization test. Optimal concentrations of guinea pig and rabbit immunoglobulins were determined by checkerboard titration. Microtitre plates were coated with 15.0 µg/ml guinea pig anti-polio immunoglobulin and rabbit anti-polio immunoglobulin at the concentration of 7.94 µg/ml was used as detecting antibody. The standard curve with eight different antigen concentrations in eight replicates resulted in a coefficient of variation (CV) between 2.1% to 7.8%. The dose-response relationship was determined by simple linear regression with a coefficient of correlation (R²) equal to 96.4%. The assay detected a minimum of 2.3 µg/ml poliovirus antigen.Instituto de Tecnologia do Paraná - Tecpar2007-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100005Brazilian Archives of Biology and Technology v.50 n.1 2007reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132007000100005info:eu-repo/semantics/openAccessHashimoto,NewtonBenati,Fabrício JoséLauretti,FlávioLinhares,Rosa Elisa CarvalhoNozawa,Carlos Mitihikoeng2007-05-30T00:00:00Zoai:scielo:S1516-89132007000100005Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2007-05-30T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Development of an enzyme immunoassay for poliovirus antigens
title Development of an enzyme immunoassay for poliovirus antigens
spellingShingle Development of an enzyme immunoassay for poliovirus antigens
Hashimoto,Newton
Enzyme immunoassay
Poliovirus antigen
title_short Development of an enzyme immunoassay for poliovirus antigens
title_full Development of an enzyme immunoassay for poliovirus antigens
title_fullStr Development of an enzyme immunoassay for poliovirus antigens
title_full_unstemmed Development of an enzyme immunoassay for poliovirus antigens
title_sort Development of an enzyme immunoassay for poliovirus antigens
author Hashimoto,Newton
author_facet Hashimoto,Newton
Benati,Fabrício José
Lauretti,Flávio
Linhares,Rosa Elisa Carvalho
Nozawa,Carlos Mitihiko
author_role author
author2 Benati,Fabrício José
Lauretti,Flávio
Linhares,Rosa Elisa Carvalho
Nozawa,Carlos Mitihiko
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Hashimoto,Newton
Benati,Fabrício José
Lauretti,Flávio
Linhares,Rosa Elisa Carvalho
Nozawa,Carlos Mitihiko
dc.subject.por.fl_str_mv Enzyme immunoassay
Poliovirus antigen
topic Enzyme immunoassay
Poliovirus antigen
description An indirect solid-phase enzyme immunoassay (EIA) was developed for the detection of poliovirus antigen. Virus antigen was obtained in LLC-MK2 cell cultures and used to prepare antibodies in rabbit and guinea pig. Antibodies were evaluated by double immunodiffusion and neutralization test. Optimal concentrations of guinea pig and rabbit immunoglobulins were determined by checkerboard titration. Microtitre plates were coated with 15.0 µg/ml guinea pig anti-polio immunoglobulin and rabbit anti-polio immunoglobulin at the concentration of 7.94 µg/ml was used as detecting antibody. The standard curve with eight different antigen concentrations in eight replicates resulted in a coefficient of variation (CV) between 2.1% to 7.8%. The dose-response relationship was determined by simple linear regression with a coefficient of correlation (R²) equal to 96.4%. The assay detected a minimum of 2.3 µg/ml poliovirus antigen.
publishDate 2007
dc.date.none.fl_str_mv 2007-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100005
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132007000100005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.50 n.1 2007
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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