Production of antisera to synthetic decapeptide of the Cooh-Terminus of rat glut2

Detalhes bibliográficos
Autor(a) principal: Vestri,Sandro
Data de Publicação: 1998
Outros Autores: Nikami,Hideki, Saito,Masayuki, Machado,Ubiratan F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000200004
Resumo: In the present study, the production of an anti-GLUT 2 antibody is reported. The antibody (S-5096) was raised in New Zealand rabbits immunized with an immunogen prepared by cross-linking the synthetic peptide and a carrier protein. A COOH-terminal decapeptide of rat GLUT 2 predicted sequence was synthesized and coupled to keyhole limpet hemocyanin, using the glutaraldehyde method. Subcellular membrane fractions were prepared from renal cortex (C) and medulla (M), and subjected to Western blotting analysis using the antiserum in a 1:200 dilution and, subsequently, [125I]protein A. As the results showed, the S-5096 anti-serum showed clear blots in kidney samples, stronger in cortex than in medulla as expected, whereas white adipose tissue and heart samples did not show any immunoreactivity. In addition, immunoblots were detected in samples prepared from duodenum and jejunum, as well as from isolated pancreatic B cells. In conclusion, the results clearly show that an anti-Glut 2 antibody, efficient enough to detect the rat protein by Western blotting analysis, was obtained.
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spelling Production of antisera to synthetic decapeptide of the Cooh-Terminus of rat glut2glucose transporterGLUT 2antibodycoupling peptideIn the present study, the production of an anti-GLUT 2 antibody is reported. The antibody (S-5096) was raised in New Zealand rabbits immunized with an immunogen prepared by cross-linking the synthetic peptide and a carrier protein. A COOH-terminal decapeptide of rat GLUT 2 predicted sequence was synthesized and coupled to keyhole limpet hemocyanin, using the glutaraldehyde method. Subcellular membrane fractions were prepared from renal cortex (C) and medulla (M), and subjected to Western blotting analysis using the antiserum in a 1:200 dilution and, subsequently, [125I]protein A. As the results showed, the S-5096 anti-serum showed clear blots in kidney samples, stronger in cortex than in medulla as expected, whereas white adipose tissue and heart samples did not show any immunoreactivity. In addition, immunoblots were detected in samples prepared from duodenum and jejunum, as well as from isolated pancreatic B cells. In conclusion, the results clearly show that an anti-Glut 2 antibody, efficient enough to detect the rat protein by Western blotting analysis, was obtained.Instituto de Tecnologia do Paraná - Tecpar1998-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000200004Brazilian Archives of Biology and Technology v.41 n.2 1998reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89131998000200004info:eu-repo/semantics/openAccessVestri,SandroNikami,HidekiSaito,MasayukiMachado,Ubiratan F.eng2011-07-27T00:00:00Zoai:scielo:S1516-89131998000200004Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2011-07-27T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Production of antisera to synthetic decapeptide of the Cooh-Terminus of rat glut2
title Production of antisera to synthetic decapeptide of the Cooh-Terminus of rat glut2
spellingShingle Production of antisera to synthetic decapeptide of the Cooh-Terminus of rat glut2
Vestri,Sandro
glucose transporter
GLUT 2
antibody
coupling peptide
title_short Production of antisera to synthetic decapeptide of the Cooh-Terminus of rat glut2
title_full Production of antisera to synthetic decapeptide of the Cooh-Terminus of rat glut2
title_fullStr Production of antisera to synthetic decapeptide of the Cooh-Terminus of rat glut2
title_full_unstemmed Production of antisera to synthetic decapeptide of the Cooh-Terminus of rat glut2
title_sort Production of antisera to synthetic decapeptide of the Cooh-Terminus of rat glut2
author Vestri,Sandro
author_facet Vestri,Sandro
Nikami,Hideki
Saito,Masayuki
Machado,Ubiratan F.
author_role author
author2 Nikami,Hideki
Saito,Masayuki
Machado,Ubiratan F.
author2_role author
author
author
dc.contributor.author.fl_str_mv Vestri,Sandro
Nikami,Hideki
Saito,Masayuki
Machado,Ubiratan F.
dc.subject.por.fl_str_mv glucose transporter
GLUT 2
antibody
coupling peptide
topic glucose transporter
GLUT 2
antibody
coupling peptide
description In the present study, the production of an anti-GLUT 2 antibody is reported. The antibody (S-5096) was raised in New Zealand rabbits immunized with an immunogen prepared by cross-linking the synthetic peptide and a carrier protein. A COOH-terminal decapeptide of rat GLUT 2 predicted sequence was synthesized and coupled to keyhole limpet hemocyanin, using the glutaraldehyde method. Subcellular membrane fractions were prepared from renal cortex (C) and medulla (M), and subjected to Western blotting analysis using the antiserum in a 1:200 dilution and, subsequently, [125I]protein A. As the results showed, the S-5096 anti-serum showed clear blots in kidney samples, stronger in cortex than in medulla as expected, whereas white adipose tissue and heart samples did not show any immunoreactivity. In addition, immunoblots were detected in samples prepared from duodenum and jejunum, as well as from isolated pancreatic B cells. In conclusion, the results clearly show that an anti-Glut 2 antibody, efficient enough to detect the rat protein by Western blotting analysis, was obtained.
publishDate 1998
dc.date.none.fl_str_mv 1998-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000200004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000200004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89131998000200004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.41 n.2 1998
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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