Bacterial adherence to different inert surfaces evaluated by epifluorescence microscopy and plate count method
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100011 |
Resumo: | The adherence of Listeria inoccua L6a and Staphylococcus aureus ATCC6538 was evaluated on stainless steel (SS), polycarbonate (PC) and polypropylene (PP) chips. The EP results showed a significant difference (p<0.05) among the microorganism species interactions, the surfaces and the contact time. The number of adhered cells on the chip surfaces increased as contact time increased and the number of L. innocua or S. aureus on the surface of SS, PC and PP chips reached 1.0x10(5) CFU/cm² after 12 h of contact, in both methods. The CP method showed a better sensitivity to detect low number of adhered cells. The EP was better when the average number of adhered cells was between 10 and 100 per microscopy field. |
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Bacterial adherence to different inert surfaces evaluated by epifluorescence microscopy and plate count methodBacterial adherenceListeria innocua and Staphylococcus aureusstainless steelpolycarbonate and polypropyleneThe adherence of Listeria inoccua L6a and Staphylococcus aureus ATCC6538 was evaluated on stainless steel (SS), polycarbonate (PC) and polypropylene (PP) chips. The EP results showed a significant difference (p<0.05) among the microorganism species interactions, the surfaces and the contact time. The number of adhered cells on the chip surfaces increased as contact time increased and the number of L. innocua or S. aureus on the surface of SS, PC and PP chips reached 1.0x10(5) CFU/cm² after 12 h of contact, in both methods. The CP method showed a better sensitivity to detect low number of adhered cells. The EP was better when the average number of adhered cells was between 10 and 100 per microscopy field.Instituto de Tecnologia do Paraná - Tecpar2004-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100011Brazilian Archives of Biology and Technology v.47 n.1 2004reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132004000100011info:eu-repo/semantics/openAccessParizzi,Silvânia Quintão FontesAndrade,Nélio José deSilva,Cleuber Antonio de SáSoares,Nilda de Fátima FerreiraSilva,Eldo Antônio Monteiro daeng2004-07-02T00:00:00Zoai:scielo:S1516-89132004000100011Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2004-07-02T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Bacterial adherence to different inert surfaces evaluated by epifluorescence microscopy and plate count method |
title |
Bacterial adherence to different inert surfaces evaluated by epifluorescence microscopy and plate count method |
spellingShingle |
Bacterial adherence to different inert surfaces evaluated by epifluorescence microscopy and plate count method Parizzi,Silvânia Quintão Fontes Bacterial adherence Listeria innocua and Staphylococcus aureus stainless steel polycarbonate and polypropylene |
title_short |
Bacterial adherence to different inert surfaces evaluated by epifluorescence microscopy and plate count method |
title_full |
Bacterial adherence to different inert surfaces evaluated by epifluorescence microscopy and plate count method |
title_fullStr |
Bacterial adherence to different inert surfaces evaluated by epifluorescence microscopy and plate count method |
title_full_unstemmed |
Bacterial adherence to different inert surfaces evaluated by epifluorescence microscopy and plate count method |
title_sort |
Bacterial adherence to different inert surfaces evaluated by epifluorescence microscopy and plate count method |
author |
Parizzi,Silvânia Quintão Fontes |
author_facet |
Parizzi,Silvânia Quintão Fontes Andrade,Nélio José de Silva,Cleuber Antonio de Sá Soares,Nilda de Fátima Ferreira Silva,Eldo Antônio Monteiro da |
author_role |
author |
author2 |
Andrade,Nélio José de Silva,Cleuber Antonio de Sá Soares,Nilda de Fátima Ferreira Silva,Eldo Antônio Monteiro da |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Parizzi,Silvânia Quintão Fontes Andrade,Nélio José de Silva,Cleuber Antonio de Sá Soares,Nilda de Fátima Ferreira Silva,Eldo Antônio Monteiro da |
dc.subject.por.fl_str_mv |
Bacterial adherence Listeria innocua and Staphylococcus aureus stainless steel polycarbonate and polypropylene |
topic |
Bacterial adherence Listeria innocua and Staphylococcus aureus stainless steel polycarbonate and polypropylene |
description |
The adherence of Listeria inoccua L6a and Staphylococcus aureus ATCC6538 was evaluated on stainless steel (SS), polycarbonate (PC) and polypropylene (PP) chips. The EP results showed a significant difference (p<0.05) among the microorganism species interactions, the surfaces and the contact time. The number of adhered cells on the chip surfaces increased as contact time increased and the number of L. innocua or S. aureus on the surface of SS, PC and PP chips reached 1.0x10(5) CFU/cm² after 12 h of contact, in both methods. The CP method showed a better sensitivity to detect low number of adhered cells. The EP was better when the average number of adhered cells was between 10 and 100 per microscopy field. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100011 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132004000100011 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.47 n.1 2004 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318269525393408 |