Effect of Permeable Cryoprotectants and Dextran in Cryopreserving Semen of Broiler Breeder Lines

• Autor(a) principal: Murugesan,Shanmugam
• Data de Publicação: 2022
• Outros Autores: Mahapatra,Ramkrishna
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Archives of Biology and Technology
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132022000100333
• topic: chicken; cryoprotectant; dextran; fertility; semen cryopreservation.

Abstract The aim of this study was to assess permeable cryoprotectants and dextran during broiler breeder lines semen cryopreservation in two separate experiments. Semen of PB-2 broiler breeder line was cryopreserved with 8% Ethylene Glycol (EG) or 6% dimethylformamide (DMF) in Beltsville Poultry Semen Extender (BPSE). In another experiment semen of Control broiler (CB) breeder line was cryopreserved with 8% EG, 8.2% dextran 10 kDa + 9% dimethylacetamide (DMA) or 8.2% dextran 20 kDa + 9% dimethylacetamide (DMA) in Sasaki diluent (SD). Semen cryopreserved in 0.5 ml plastic straw was thawed either at 5°C for 100 sec or 37°C for 30 sec. Semen was assessed for motility, live sperm, abnormal sperm and acrosome intact sperm. Post-thaw semen was inseminated, eggs were collected and incubated for obtaining fertility and hatchability data. The cryopreserved samples had significantly (p<0.05) lower sperm motility, live sperm and acrosome intact sperm in both the breeder lines. Moderate fertility was obtained in PB-2 line with no difference between the two permeable cryoprotectants evaluated. The fertility was significantly (p <0.05) lower using 8% EG and 8.2% dextran 20 kDa + 9% DMA in CB line. Permeable cryoprotectants (8% EG or 6% DMF) produced acceptable fertility during PB-2 semen cryopreservation. The permeable cryoprotectant 8% EG or dextran in combination with DMA gave very low fertility in CB line.