Phytoene Synthase Gene ( PSY ) from Sweet Potato ( Ipomoea batatas Lam . ) Enhances Tolerance to Abiotic Stress
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100422 |
Resumo: | ABSTRACT Phytoene synthase (PSY) is the rate-limiting enzyme for carotenoid biosynthesis. To date, several studies focused on PSY genes in the context of abiotic stress responses. In this study, two phytoene synthase encoding genes, IbPSY1 and IbPSY2, were identified from a published transcriptome and bioinformatic analysis showed that they shared conserved domains with phytoene synthases from other plants. The IbPSY1 gene was cloned and carefully characterized. Digital gene expression profiling (DGE) showed that the highest transcription level of IbPSY1 was in young leaves, and the lowest level was in stems. In vivo expression levels of IbPSY1 under abiotic stress were observed to be highest in stems at day 11. Over-expression of IbPSY1 in Escherichia coli and yeast cells endowed the cells with better growth under salt and drought stress than the control cells. This study demonstrated that IbPSY1 not only played an important role in vivo, but also in E. coli and yeast to improve tolerance to salinity and drought stress. Thus, IbPSY1 may be aid in the development of transgenic plants with enhanced stress tolerance. |
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Brazilian Archives of Biology and Technology |
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Phytoene Synthase Gene ( PSY ) from Sweet Potato ( Ipomoea batatas Lam . ) Enhances Tolerance to Abiotic StressIpomoea batatasphytoene synthaseabiotic stressABSTRACT Phytoene synthase (PSY) is the rate-limiting enzyme for carotenoid biosynthesis. To date, several studies focused on PSY genes in the context of abiotic stress responses. In this study, two phytoene synthase encoding genes, IbPSY1 and IbPSY2, were identified from a published transcriptome and bioinformatic analysis showed that they shared conserved domains with phytoene synthases from other plants. The IbPSY1 gene was cloned and carefully characterized. Digital gene expression profiling (DGE) showed that the highest transcription level of IbPSY1 was in young leaves, and the lowest level was in stems. In vivo expression levels of IbPSY1 under abiotic stress were observed to be highest in stems at day 11. Over-expression of IbPSY1 in Escherichia coli and yeast cells endowed the cells with better growth under salt and drought stress than the control cells. This study demonstrated that IbPSY1 not only played an important role in vivo, but also in E. coli and yeast to improve tolerance to salinity and drought stress. Thus, IbPSY1 may be aid in the development of transgenic plants with enhanced stress tolerance.Instituto de Tecnologia do Paraná - Tecpar2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100422Brazilian Archives of Biology and Technology v.61 2018reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/1678-4324-2018160558info:eu-repo/semantics/openAccessShao,HuanhuanYong,BinXu,PanZheng,HaiyanLiao,RuoxingWang,XiaoyanLi,XinyuZhang,LiShen,Jiabineng2018-11-07T00:00:00Zoai:scielo:S1516-89132018000100422Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2018-11-07T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Phytoene Synthase Gene ( PSY ) from Sweet Potato ( Ipomoea batatas Lam . ) Enhances Tolerance to Abiotic Stress |
title |
Phytoene Synthase Gene ( PSY ) from Sweet Potato ( Ipomoea batatas Lam . ) Enhances Tolerance to Abiotic Stress |
spellingShingle |
Phytoene Synthase Gene ( PSY ) from Sweet Potato ( Ipomoea batatas Lam . ) Enhances Tolerance to Abiotic Stress Shao,Huanhuan Ipomoea batatas phytoene synthase abiotic stress |
title_short |
Phytoene Synthase Gene ( PSY ) from Sweet Potato ( Ipomoea batatas Lam . ) Enhances Tolerance to Abiotic Stress |
title_full |
Phytoene Synthase Gene ( PSY ) from Sweet Potato ( Ipomoea batatas Lam . ) Enhances Tolerance to Abiotic Stress |
title_fullStr |
Phytoene Synthase Gene ( PSY ) from Sweet Potato ( Ipomoea batatas Lam . ) Enhances Tolerance to Abiotic Stress |
title_full_unstemmed |
Phytoene Synthase Gene ( PSY ) from Sweet Potato ( Ipomoea batatas Lam . ) Enhances Tolerance to Abiotic Stress |
title_sort |
Phytoene Synthase Gene ( PSY ) from Sweet Potato ( Ipomoea batatas Lam . ) Enhances Tolerance to Abiotic Stress |
author |
Shao,Huanhuan |
author_facet |
Shao,Huanhuan Yong,Bin Xu,Pan Zheng,Haiyan Liao,Ruoxing Wang,Xiaoyan Li,Xinyu Zhang,Li Shen,Jiabin |
author_role |
author |
author2 |
Yong,Bin Xu,Pan Zheng,Haiyan Liao,Ruoxing Wang,Xiaoyan Li,Xinyu Zhang,Li Shen,Jiabin |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Shao,Huanhuan Yong,Bin Xu,Pan Zheng,Haiyan Liao,Ruoxing Wang,Xiaoyan Li,Xinyu Zhang,Li Shen,Jiabin |
dc.subject.por.fl_str_mv |
Ipomoea batatas phytoene synthase abiotic stress |
topic |
Ipomoea batatas phytoene synthase abiotic stress |
description |
ABSTRACT Phytoene synthase (PSY) is the rate-limiting enzyme for carotenoid biosynthesis. To date, several studies focused on PSY genes in the context of abiotic stress responses. In this study, two phytoene synthase encoding genes, IbPSY1 and IbPSY2, were identified from a published transcriptome and bioinformatic analysis showed that they shared conserved domains with phytoene synthases from other plants. The IbPSY1 gene was cloned and carefully characterized. Digital gene expression profiling (DGE) showed that the highest transcription level of IbPSY1 was in young leaves, and the lowest level was in stems. In vivo expression levels of IbPSY1 under abiotic stress were observed to be highest in stems at day 11. Over-expression of IbPSY1 in Escherichia coli and yeast cells endowed the cells with better growth under salt and drought stress than the control cells. This study demonstrated that IbPSY1 not only played an important role in vivo, but also in E. coli and yeast to improve tolerance to salinity and drought stress. Thus, IbPSY1 may be aid in the development of transgenic plants with enhanced stress tolerance. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100422 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100422 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-4324-2018160558 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.61 2018 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318278677364736 |