The effect of concanavalin A on the replication of rotavirus (SA-11) in cell culture

Detalhes bibliográficos
Autor(a) principal: Hasenack,Beatriz S.
Data de Publicação: 2002
Outros Autores: Botelho,Maria Valéria J., Lauretti,Flávio, Melo,Fernando L. de, Orlandi,Janaina M., Linhares,Rosa Elisa C., Ueda,Marli, Nozawa,Carlos M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000200003
Resumo: Rotavirus (RV) strain SA-11 was studied with respect to its infectivity in MA-104 cell cultures and the effect of concanavalin A (ConA). Receptors for ConA at the surface of MA-104 cell were determined by fluorescence assay and specifically inhibited by D-mannose. The kinetics of virus growth was carried out by plaque assay. Electron microscopy and polyacrylamide gel electrophoresis were used for monitoring the experiments. It was concluded that RV replication was not affected consistently by ConA, however it interfered with the development of cytopathic effect (CPE) without altering virus yields.
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spelling The effect of concanavalin A on the replication of rotavirus (SA-11) in cell cultureRotavirusConcanavalin ACell cultureRotavirus (RV) strain SA-11 was studied with respect to its infectivity in MA-104 cell cultures and the effect of concanavalin A (ConA). Receptors for ConA at the surface of MA-104 cell were determined by fluorescence assay and specifically inhibited by D-mannose. The kinetics of virus growth was carried out by plaque assay. Electron microscopy and polyacrylamide gel electrophoresis were used for monitoring the experiments. It was concluded that RV replication was not affected consistently by ConA, however it interfered with the development of cytopathic effect (CPE) without altering virus yields.Instituto de Tecnologia do Paraná - Tecpar2002-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000200003Brazilian Archives of Biology and Technology v.45 n.2 2002reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132002000200003info:eu-repo/semantics/openAccessHasenack,Beatriz S.Botelho,Maria Valéria J.Lauretti,FlávioMelo,Fernando L. deOrlandi,Janaina M.Linhares,Rosa Elisa C.Ueda,MarliNozawa,Carlos M.eng2002-09-27T00:00:00Zoai:scielo:S1516-89132002000200003Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2002-09-27T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv The effect of concanavalin A on the replication of rotavirus (SA-11) in cell culture
title The effect of concanavalin A on the replication of rotavirus (SA-11) in cell culture
spellingShingle The effect of concanavalin A on the replication of rotavirus (SA-11) in cell culture
Hasenack,Beatriz S.
Rotavirus
Concanavalin A
Cell culture
title_short The effect of concanavalin A on the replication of rotavirus (SA-11) in cell culture
title_full The effect of concanavalin A on the replication of rotavirus (SA-11) in cell culture
title_fullStr The effect of concanavalin A on the replication of rotavirus (SA-11) in cell culture
title_full_unstemmed The effect of concanavalin A on the replication of rotavirus (SA-11) in cell culture
title_sort The effect of concanavalin A on the replication of rotavirus (SA-11) in cell culture
author Hasenack,Beatriz S.
author_facet Hasenack,Beatriz S.
Botelho,Maria Valéria J.
Lauretti,Flávio
Melo,Fernando L. de
Orlandi,Janaina M.
Linhares,Rosa Elisa C.
Ueda,Marli
Nozawa,Carlos M.
author_role author
author2 Botelho,Maria Valéria J.
Lauretti,Flávio
Melo,Fernando L. de
Orlandi,Janaina M.
Linhares,Rosa Elisa C.
Ueda,Marli
Nozawa,Carlos M.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Hasenack,Beatriz S.
Botelho,Maria Valéria J.
Lauretti,Flávio
Melo,Fernando L. de
Orlandi,Janaina M.
Linhares,Rosa Elisa C.
Ueda,Marli
Nozawa,Carlos M.
dc.subject.por.fl_str_mv Rotavirus
Concanavalin A
Cell culture
topic Rotavirus
Concanavalin A
Cell culture
description Rotavirus (RV) strain SA-11 was studied with respect to its infectivity in MA-104 cell cultures and the effect of concanavalin A (ConA). Receptors for ConA at the surface of MA-104 cell were determined by fluorescence assay and specifically inhibited by D-mannose. The kinetics of virus growth was carried out by plaque assay. Electron microscopy and polyacrylamide gel electrophoresis were used for monitoring the experiments. It was concluded that RV replication was not affected consistently by ConA, however it interfered with the development of cytopathic effect (CPE) without altering virus yields.
publishDate 2002
dc.date.none.fl_str_mv 2002-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000200003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000200003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132002000200003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.45 n.2 2002
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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