Recovery of ascorbic oxidoreductase from crude extract with an aqueous two-phase system in a perforated rotating disc contactor

• Autor(a) principal: Porto,Ana Lúcia Figueiredo
• Data de Publicação: 2004
• Outros Autores: Sarubbo,Leonie Asfora, Moreira,Keila Aparecida, Melo,Homero José Farias de, Lima-Filho,José Luís, Campos-Takaki,Galba Maria, Tambourgi,Elias Basile
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Archives of Biology and Technology
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500019
• topic: Efficiency; mass transfer coefficient; Perforated Rotating Disc contactor; aqueous two-phase system; ascorbic oxidoreductase

A continuous perforated rotating disc contactor was used to extract the enzyme ascorbic oxidoreductase (E.C.1.10.3.3) from crude extract of Curcubita maxima with an aqueous two-phase system of poly (ethylene glycol) and phosphate salts. The effect of dispersed phase velocity on either protein mass transfer coefficients or separation efficiency at 1, 2 and 3 mL/min was studied. An increase of the mass transfer coefficients was observed with the dispersed phase velocity, while the separation efficiency showed a small decrease with the increase of this parameter. The experimental results obtained during continuous extraction showed that the ascorbic oxidoreductase activity was partitioned preferentially into the salt-rich phase in all conditions studied. The best recovery of enzyme activity was 236%, with a purification factor of 34 in flow rates of 1 mL/min for dispersed phase.