Detection of Streptococcus mutans using padlock probe based on Rolling Circle Amplification (RCA)

Detalhes bibliográficos
Autor(a) principal: Moreira,Mônica
Data de Publicação: 2015
Outros Autores: Adamoski,Douglas, Sun,Jiufeng, Najafzadeh,Mohammad Javad, Nascimento,Mariana Machado Fidelis do, Gomes,Renata Rodrigues, Barbieri,Dicler de Sant'Anna, Glienke,Chirlei, Klisiowicz,Débora do Rocio, Vicente,Vânia Aparecida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100054
Resumo: The aim of this study was to develop and evaluate a padlock probe based on the Rolling Circle Amplification (RCA), which targeted to 16S-23S rDNA region of S. mutans. The specificity of developed padlock probe was tested for DNA within a panel strains, including S. mutans isolated from the saliva and reference strains of the genus Streptococcus, as well as total DNA samples of biofilm and saliva. The results were positive either for DNA samples of S. mutans or DNA samples recovered from the biofilm and saliva revealing the specificity of designed padlock probe. The padlock probe based on the RCA was proved to be an effective, reproducible method for S. mutansdetection and demonstrated the possibility of a rapid detection and accurate identification of S. mutans infection.
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spelling Detection of Streptococcus mutans using padlock probe based on Rolling Circle Amplification (RCA)Padlock probeRCAStreptococcus mutansThe aim of this study was to develop and evaluate a padlock probe based on the Rolling Circle Amplification (RCA), which targeted to 16S-23S rDNA region of S. mutans. The specificity of developed padlock probe was tested for DNA within a panel strains, including S. mutans isolated from the saliva and reference strains of the genus Streptococcus, as well as total DNA samples of biofilm and saliva. The results were positive either for DNA samples of S. mutans or DNA samples recovered from the biofilm and saliva revealing the specificity of designed padlock probe. The padlock probe based on the RCA was proved to be an effective, reproducible method for S. mutansdetection and demonstrated the possibility of a rapid detection and accurate identification of S. mutans infection.Instituto de Tecnologia do Paraná - Tecpar2015-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100054Brazilian Archives of Biology and Technology v.58 n.1 2015reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-8913201502762info:eu-repo/semantics/openAccessMoreira,MônicaAdamoski,DouglasSun,JiufengNajafzadeh,Mohammad JavadNascimento,Mariana Machado Fidelis doGomes,Renata RodriguesBarbieri,Dicler de Sant'AnnaGlienke,ChirleiKlisiowicz,Débora do RocioVicente,Vânia Aparecidaeng2015-10-26T00:00:00Zoai:scielo:S1516-89132015000100054Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2015-10-26T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Detection of Streptococcus mutans using padlock probe based on Rolling Circle Amplification (RCA)
title Detection of Streptococcus mutans using padlock probe based on Rolling Circle Amplification (RCA)
spellingShingle Detection of Streptococcus mutans using padlock probe based on Rolling Circle Amplification (RCA)
Moreira,Mônica
Padlock probe
RCA
Streptococcus mutans
title_short Detection of Streptococcus mutans using padlock probe based on Rolling Circle Amplification (RCA)
title_full Detection of Streptococcus mutans using padlock probe based on Rolling Circle Amplification (RCA)
title_fullStr Detection of Streptococcus mutans using padlock probe based on Rolling Circle Amplification (RCA)
title_full_unstemmed Detection of Streptococcus mutans using padlock probe based on Rolling Circle Amplification (RCA)
title_sort Detection of Streptococcus mutans using padlock probe based on Rolling Circle Amplification (RCA)
author Moreira,Mônica
author_facet Moreira,Mônica
Adamoski,Douglas
Sun,Jiufeng
Najafzadeh,Mohammad Javad
Nascimento,Mariana Machado Fidelis do
Gomes,Renata Rodrigues
Barbieri,Dicler de Sant'Anna
Glienke,Chirlei
Klisiowicz,Débora do Rocio
Vicente,Vânia Aparecida
author_role author
author2 Adamoski,Douglas
Sun,Jiufeng
Najafzadeh,Mohammad Javad
Nascimento,Mariana Machado Fidelis do
Gomes,Renata Rodrigues
Barbieri,Dicler de Sant'Anna
Glienke,Chirlei
Klisiowicz,Débora do Rocio
Vicente,Vânia Aparecida
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Moreira,Mônica
Adamoski,Douglas
Sun,Jiufeng
Najafzadeh,Mohammad Javad
Nascimento,Mariana Machado Fidelis do
Gomes,Renata Rodrigues
Barbieri,Dicler de Sant'Anna
Glienke,Chirlei
Klisiowicz,Débora do Rocio
Vicente,Vânia Aparecida
dc.subject.por.fl_str_mv Padlock probe
RCA
Streptococcus mutans
topic Padlock probe
RCA
Streptococcus mutans
description The aim of this study was to develop and evaluate a padlock probe based on the Rolling Circle Amplification (RCA), which targeted to 16S-23S rDNA region of S. mutans. The specificity of developed padlock probe was tested for DNA within a panel strains, including S. mutans isolated from the saliva and reference strains of the genus Streptococcus, as well as total DNA samples of biofilm and saliva. The results were positive either for DNA samples of S. mutans or DNA samples recovered from the biofilm and saliva revealing the specificity of designed padlock probe. The padlock probe based on the RCA was proved to be an effective, reproducible method for S. mutansdetection and demonstrated the possibility of a rapid detection and accurate identification of S. mutans infection.
publishDate 2015
dc.date.none.fl_str_mv 2015-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100054
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100054
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-8913201502762
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.58 n.1 2015
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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