Induction of leafy galls in Acacia mearnsii De Wild seedlings infected by Rhodococcus fascians
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300002 |
Resumo: | Plantlets of blackwattle (Acacia mearnsii De Wild) were inoculated with the bacterium Rhodococcus fascians and cultured in vitro. Leafy galls appeared at the cotyledonary nodes in 75% of the infected plants. The galls were separated from the plants and cultured on a medium containing three-quarters-strength MS salts (Murashige and Skoog, 1962), MS vitamins, 2% sucrose and an antibiotic (cephalothin), supplemented with or without 0.2% activated charcoal. Histological studies conducted from the sixth to the twenty-second day after plant infection revealed the presence of newly formed meristematic centers, first in the axillary region, then on the petioles and lamina of the leaflets around the apical meristem. Approximately 37% of the galls developed one shoot with both concentrations of cephalothin. |
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Brazilian Archives of Biology and Technology |
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Induction of leafy galls in Acacia mearnsii De Wild seedlings infected by Rhodococcus fasciansBlack wattlelegume treemicropropagationPlantlets of blackwattle (Acacia mearnsii De Wild) were inoculated with the bacterium Rhodococcus fascians and cultured in vitro. Leafy galls appeared at the cotyledonary nodes in 75% of the infected plants. The galls were separated from the plants and cultured on a medium containing three-quarters-strength MS salts (Murashige and Skoog, 1962), MS vitamins, 2% sucrose and an antibiotic (cephalothin), supplemented with or without 0.2% activated charcoal. Histological studies conducted from the sixth to the twenty-second day after plant infection revealed the presence of newly formed meristematic centers, first in the axillary region, then on the petioles and lamina of the leaflets around the apical meristem. Approximately 37% of the galls developed one shoot with both concentrations of cephalothin.Instituto de Tecnologia do Paraná - Tecpar2004-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300002Brazilian Archives of Biology and Technology v.47 n.3 2004reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132004000300002info:eu-repo/semantics/openAccessQuoirin,MargueriteBona,CleusaSouza,Elizandra F. deSchwartsburd,Pedro B.eng2004-08-03T00:00:00Zoai:scielo:S1516-89132004000300002Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2004-08-03T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Induction of leafy galls in Acacia mearnsii De Wild seedlings infected by Rhodococcus fascians |
title |
Induction of leafy galls in Acacia mearnsii De Wild seedlings infected by Rhodococcus fascians |
spellingShingle |
Induction of leafy galls in Acacia mearnsii De Wild seedlings infected by Rhodococcus fascians Quoirin,Marguerite Black wattle legume tree micropropagation |
title_short |
Induction of leafy galls in Acacia mearnsii De Wild seedlings infected by Rhodococcus fascians |
title_full |
Induction of leafy galls in Acacia mearnsii De Wild seedlings infected by Rhodococcus fascians |
title_fullStr |
Induction of leafy galls in Acacia mearnsii De Wild seedlings infected by Rhodococcus fascians |
title_full_unstemmed |
Induction of leafy galls in Acacia mearnsii De Wild seedlings infected by Rhodococcus fascians |
title_sort |
Induction of leafy galls in Acacia mearnsii De Wild seedlings infected by Rhodococcus fascians |
author |
Quoirin,Marguerite |
author_facet |
Quoirin,Marguerite Bona,Cleusa Souza,Elizandra F. de Schwartsburd,Pedro B. |
author_role |
author |
author2 |
Bona,Cleusa Souza,Elizandra F. de Schwartsburd,Pedro B. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Quoirin,Marguerite Bona,Cleusa Souza,Elizandra F. de Schwartsburd,Pedro B. |
dc.subject.por.fl_str_mv |
Black wattle legume tree micropropagation |
topic |
Black wattle legume tree micropropagation |
description |
Plantlets of blackwattle (Acacia mearnsii De Wild) were inoculated with the bacterium Rhodococcus fascians and cultured in vitro. Leafy galls appeared at the cotyledonary nodes in 75% of the infected plants. The galls were separated from the plants and cultured on a medium containing three-quarters-strength MS salts (Murashige and Skoog, 1962), MS vitamins, 2% sucrose and an antibiotic (cephalothin), supplemented with or without 0.2% activated charcoal. Histological studies conducted from the sixth to the twenty-second day after plant infection revealed the presence of newly formed meristematic centers, first in the axillary region, then on the petioles and lamina of the leaflets around the apical meristem. Approximately 37% of the galls developed one shoot with both concentrations of cephalothin. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-07-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300002 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132004000300002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.47 n.3 2004 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318269836820480 |