Detection and identification of TMV infecting tomato under protected cultivation in Paraná State
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500005 |
Resumo: | During an inspection in plastic houses in Sapopema, Paraná, 90% of tomato plants showed leaf abnormalities, probably associated with herbicide toxity. However, virus like symptoms developed in selected hosts after mechanical inoculatation. RT-PCR reactions using primers for an internal region within the movement protein gene of TMV and ToMV resulted in the amplification of a 409 bp cDNA fragment only by TMV primers. Deduced amino acids showed 100% identity when compared to TMV movement protein and 94% with ToMV. The RT-PCR protocol was efficient for quick and conclusive determination of virus species. The virus was purified and a polyclonal antiserum was raised for future surveys in tomato crops of Paraná. The partial genomic sequence obtained for TMV-Sapopema has been deposited under the accession number DQ173945, which is the first partial genomic sequence of an isolate of TMV from Brazil in the GenBank, and the first tomato virus isolate from Paraná to have some of its biological and molecular properties determined. |
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Detection and identification of TMV infecting tomato under protected cultivation in Paraná StateTMVP30 proteinplasticultureDuring an inspection in plastic houses in Sapopema, Paraná, 90% of tomato plants showed leaf abnormalities, probably associated with herbicide toxity. However, virus like symptoms developed in selected hosts after mechanical inoculatation. RT-PCR reactions using primers for an internal region within the movement protein gene of TMV and ToMV resulted in the amplification of a 409 bp cDNA fragment only by TMV primers. Deduced amino acids showed 100% identity when compared to TMV movement protein and 94% with ToMV. The RT-PCR protocol was efficient for quick and conclusive determination of virus species. The virus was purified and a polyclonal antiserum was raised for future surveys in tomato crops of Paraná. The partial genomic sequence obtained for TMV-Sapopema has been deposited under the accession number DQ173945, which is the first partial genomic sequence of an isolate of TMV from Brazil in the GenBank, and the first tomato virus isolate from Paraná to have some of its biological and molecular properties determined.Instituto de Tecnologia do Paraná - Tecpar2008-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500005Brazilian Archives of Biology and Technology v.51 n.5 2008reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132008000500005info:eu-repo/semantics/openAccessSilva,Rodrigo Martins daSouto,Eliezer Rodrigues dePedroso,Júlio CézarArakava,RicardoAlmeida,Álvaro Manuel RodriguesBarboza,Antônio Augusto LazariniVida,João Batistaeng2008-10-29T00:00:00Zoai:scielo:S1516-89132008000500005Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2008-10-29T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Detection and identification of TMV infecting tomato under protected cultivation in Paraná State |
title |
Detection and identification of TMV infecting tomato under protected cultivation in Paraná State |
spellingShingle |
Detection and identification of TMV infecting tomato under protected cultivation in Paraná State Silva,Rodrigo Martins da TMV P30 protein plasticulture |
title_short |
Detection and identification of TMV infecting tomato under protected cultivation in Paraná State |
title_full |
Detection and identification of TMV infecting tomato under protected cultivation in Paraná State |
title_fullStr |
Detection and identification of TMV infecting tomato under protected cultivation in Paraná State |
title_full_unstemmed |
Detection and identification of TMV infecting tomato under protected cultivation in Paraná State |
title_sort |
Detection and identification of TMV infecting tomato under protected cultivation in Paraná State |
author |
Silva,Rodrigo Martins da |
author_facet |
Silva,Rodrigo Martins da Souto,Eliezer Rodrigues de Pedroso,Júlio Cézar Arakava,Ricardo Almeida,Álvaro Manuel Rodrigues Barboza,Antônio Augusto Lazarini Vida,João Batista |
author_role |
author |
author2 |
Souto,Eliezer Rodrigues de Pedroso,Júlio Cézar Arakava,Ricardo Almeida,Álvaro Manuel Rodrigues Barboza,Antônio Augusto Lazarini Vida,João Batista |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Silva,Rodrigo Martins da Souto,Eliezer Rodrigues de Pedroso,Júlio Cézar Arakava,Ricardo Almeida,Álvaro Manuel Rodrigues Barboza,Antônio Augusto Lazarini Vida,João Batista |
dc.subject.por.fl_str_mv |
TMV P30 protein plasticulture |
topic |
TMV P30 protein plasticulture |
description |
During an inspection in plastic houses in Sapopema, Paraná, 90% of tomato plants showed leaf abnormalities, probably associated with herbicide toxity. However, virus like symptoms developed in selected hosts after mechanical inoculatation. RT-PCR reactions using primers for an internal region within the movement protein gene of TMV and ToMV resulted in the amplification of a 409 bp cDNA fragment only by TMV primers. Deduced amino acids showed 100% identity when compared to TMV movement protein and 94% with ToMV. The RT-PCR protocol was efficient for quick and conclusive determination of virus species. The virus was purified and a polyclonal antiserum was raised for future surveys in tomato crops of Paraná. The partial genomic sequence obtained for TMV-Sapopema has been deposited under the accession number DQ173945, which is the first partial genomic sequence of an isolate of TMV from Brazil in the GenBank, and the first tomato virus isolate from Paraná to have some of its biological and molecular properties determined. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132008000500005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.51 n.5 2008 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318272316702720 |