Characteristics of Immobilized Urease on Grafted Alginate Bead Systems

Detalhes bibliográficos
Autor(a) principal: Danial,Enas N.
Data de Publicação: 2015
Outros Autores: Hamza,Amal H., Mahmoud,Rasha H.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200147
Resumo: This study evaluated the biological importance of immobilized urease enzyme over the free urease. The support material used for urease immobilization was alginate. Generally, the immobilization of urease in alginate gel showed a marked increase in Km and Vmax. However, the immobilized urease showed higher thermal stability than that of free enzyme. The rate of thermal inactivation of the immobilized enzyme decreased due to entrapment in gel matrix. Also, the activity of the immobilized urease was more stable in retention than that of the free enzyme during the storage in solution, although the activity of the immobilized enzyme was lower in comparison with the free enzyme. A stable immobilized system and long storage life are convenient for applications that would not be feasible with a soluble enzyme system. These results highlighted the technical and biochemical benefits of immobilized urease over the free enzyme.
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spelling Characteristics of Immobilized Urease on Grafted Alginate Bead SystemsUreaseimmobilizationKineticreusabilitystorage stabilityThis study evaluated the biological importance of immobilized urease enzyme over the free urease. The support material used for urease immobilization was alginate. Generally, the immobilization of urease in alginate gel showed a marked increase in Km and Vmax. However, the immobilized urease showed higher thermal stability than that of free enzyme. The rate of thermal inactivation of the immobilized enzyme decreased due to entrapment in gel matrix. Also, the activity of the immobilized urease was more stable in retention than that of the free enzyme during the storage in solution, although the activity of the immobilized enzyme was lower in comparison with the free enzyme. A stable immobilized system and long storage life are convenient for applications that would not be feasible with a soluble enzyme system. These results highlighted the technical and biochemical benefits of immobilized urease over the free enzyme.Instituto de Tecnologia do Paraná - Tecpar2015-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200147Brazilian Archives of Biology and Technology v.58 n.2 2015reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-8913201400204info:eu-repo/semantics/openAccessDanial,Enas N.Hamza,Amal H.Mahmoud,Rasha H.eng2015-10-08T00:00:00Zoai:scielo:S1516-89132015000200147Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2015-10-08T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Characteristics of Immobilized Urease on Grafted Alginate Bead Systems
title Characteristics of Immobilized Urease on Grafted Alginate Bead Systems
spellingShingle Characteristics of Immobilized Urease on Grafted Alginate Bead Systems
Danial,Enas N.
Urease
immobilization
Kinetic
reusability
storage stability
title_short Characteristics of Immobilized Urease on Grafted Alginate Bead Systems
title_full Characteristics of Immobilized Urease on Grafted Alginate Bead Systems
title_fullStr Characteristics of Immobilized Urease on Grafted Alginate Bead Systems
title_full_unstemmed Characteristics of Immobilized Urease on Grafted Alginate Bead Systems
title_sort Characteristics of Immobilized Urease on Grafted Alginate Bead Systems
author Danial,Enas N.
author_facet Danial,Enas N.
Hamza,Amal H.
Mahmoud,Rasha H.
author_role author
author2 Hamza,Amal H.
Mahmoud,Rasha H.
author2_role author
author
dc.contributor.author.fl_str_mv Danial,Enas N.
Hamza,Amal H.
Mahmoud,Rasha H.
dc.subject.por.fl_str_mv Urease
immobilization
Kinetic
reusability
storage stability
topic Urease
immobilization
Kinetic
reusability
storage stability
description This study evaluated the biological importance of immobilized urease enzyme over the free urease. The support material used for urease immobilization was alginate. Generally, the immobilization of urease in alginate gel showed a marked increase in Km and Vmax. However, the immobilized urease showed higher thermal stability than that of free enzyme. The rate of thermal inactivation of the immobilized enzyme decreased due to entrapment in gel matrix. Also, the activity of the immobilized urease was more stable in retention than that of the free enzyme during the storage in solution, although the activity of the immobilized enzyme was lower in comparison with the free enzyme. A stable immobilized system and long storage life are convenient for applications that would not be feasible with a soluble enzyme system. These results highlighted the technical and biochemical benefits of immobilized urease over the free enzyme.
publishDate 2015
dc.date.none.fl_str_mv 2015-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200147
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200147
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-8913201400204
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.58 n.2 2015
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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