Evaluation of Genetic Diversity amongthe Pakistani Wheat (Triticumaestivum L.) Lines through Random Molecular Markers
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100432 |
Resumo: | ABSTRACT The presence of geneticdiversity is of great importance in improving wheat traits and developing strategies for optimal conservation of germplasm. Genetic diversity was assessed among common wheat cultivars using RAPD (Random Amplified Polymorphic DNA) markers at the Center of Agriculture, Biochemistry and Biotechnology (CABB), University of Agriculture, Faisalabad. RAPD primers were used among 14 Pakistani wheat cultivars, to screen the progenies and for the identification of the genes of interest. The polymorphic information content (PIC), was measured as the percentage of polymorphic fragments for all primers. A total of 583 bands(84% polymorphic) in all 14 wheat cultivars was amplified and discriminated all the wheat genotypes. The number of fragments amplified per primer ranged from 35 to 69 with an average of 48.52 fragments per primer averagely was observed. Population structure analysis anddandrogram showed distinct clustering among different wheat genotypes. Millat-11, Punjab-11, PBW-222 generated the maximum level of polymorphism, standing alone in the cluster while others are scatteredin different group.As a result, genetically numerous progenies are known, increasing the quality of sorts collections by broadening the genetic base of wheat cultivars. This study additionally indicates that RAPD markers allow quicker response and provide high throughputprocedure of accessions from a variety assortment to assess genetic diversity among wheat genotypes. |
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Brazilian Archives of Biology and Technology |
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Evaluation of Genetic Diversity amongthe Pakistani Wheat (Triticumaestivum L.) Lines through Random Molecular MarkersWheatRAPDgenetic diversityPCoACluster analysisABSTRACT The presence of geneticdiversity is of great importance in improving wheat traits and developing strategies for optimal conservation of germplasm. Genetic diversity was assessed among common wheat cultivars using RAPD (Random Amplified Polymorphic DNA) markers at the Center of Agriculture, Biochemistry and Biotechnology (CABB), University of Agriculture, Faisalabad. RAPD primers were used among 14 Pakistani wheat cultivars, to screen the progenies and for the identification of the genes of interest. The polymorphic information content (PIC), was measured as the percentage of polymorphic fragments for all primers. A total of 583 bands(84% polymorphic) in all 14 wheat cultivars was amplified and discriminated all the wheat genotypes. The number of fragments amplified per primer ranged from 35 to 69 with an average of 48.52 fragments per primer averagely was observed. Population structure analysis anddandrogram showed distinct clustering among different wheat genotypes. Millat-11, Punjab-11, PBW-222 generated the maximum level of polymorphism, standing alone in the cluster while others are scatteredin different group.As a result, genetically numerous progenies are known, increasing the quality of sorts collections by broadening the genetic base of wheat cultivars. This study additionally indicates that RAPD markers allow quicker response and provide high throughputprocedure of accessions from a variety assortment to assess genetic diversity among wheat genotypes.Instituto de Tecnologia do Paraná - Tecpar2016-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100432Brazilian Archives of Biology and Technology v.59 2016reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/1678-4324-2016160282info:eu-repo/semantics/openAccessZeshan,AliAfzal,MuhammadAlghamdi,Salem S.Kettener,KarienAli,MubasharMubushar,MuhammadAhmad,Shakeeleng2018-06-27T00:00:00Zoai:scielo:S1516-89132016000100432Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2018-06-27T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Evaluation of Genetic Diversity amongthe Pakistani Wheat (Triticumaestivum L.) Lines through Random Molecular Markers |
title |
Evaluation of Genetic Diversity amongthe Pakistani Wheat (Triticumaestivum L.) Lines through Random Molecular Markers |
spellingShingle |
Evaluation of Genetic Diversity amongthe Pakistani Wheat (Triticumaestivum L.) Lines through Random Molecular Markers Zeshan,Ali Wheat RAPD genetic diversity PCoA Cluster analysis |
title_short |
Evaluation of Genetic Diversity amongthe Pakistani Wheat (Triticumaestivum L.) Lines through Random Molecular Markers |
title_full |
Evaluation of Genetic Diversity amongthe Pakistani Wheat (Triticumaestivum L.) Lines through Random Molecular Markers |
title_fullStr |
Evaluation of Genetic Diversity amongthe Pakistani Wheat (Triticumaestivum L.) Lines through Random Molecular Markers |
title_full_unstemmed |
Evaluation of Genetic Diversity amongthe Pakistani Wheat (Triticumaestivum L.) Lines through Random Molecular Markers |
title_sort |
Evaluation of Genetic Diversity amongthe Pakistani Wheat (Triticumaestivum L.) Lines through Random Molecular Markers |
author |
Zeshan,Ali |
author_facet |
Zeshan,Ali Afzal,Muhammad Alghamdi,Salem S. Kettener,Karien Ali,Mubashar Mubushar,Muhammad Ahmad,Shakeel |
author_role |
author |
author2 |
Afzal,Muhammad Alghamdi,Salem S. Kettener,Karien Ali,Mubashar Mubushar,Muhammad Ahmad,Shakeel |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Zeshan,Ali Afzal,Muhammad Alghamdi,Salem S. Kettener,Karien Ali,Mubashar Mubushar,Muhammad Ahmad,Shakeel |
dc.subject.por.fl_str_mv |
Wheat RAPD genetic diversity PCoA Cluster analysis |
topic |
Wheat RAPD genetic diversity PCoA Cluster analysis |
description |
ABSTRACT The presence of geneticdiversity is of great importance in improving wheat traits and developing strategies for optimal conservation of germplasm. Genetic diversity was assessed among common wheat cultivars using RAPD (Random Amplified Polymorphic DNA) markers at the Center of Agriculture, Biochemistry and Biotechnology (CABB), University of Agriculture, Faisalabad. RAPD primers were used among 14 Pakistani wheat cultivars, to screen the progenies and for the identification of the genes of interest. The polymorphic information content (PIC), was measured as the percentage of polymorphic fragments for all primers. A total of 583 bands(84% polymorphic) in all 14 wheat cultivars was amplified and discriminated all the wheat genotypes. The number of fragments amplified per primer ranged from 35 to 69 with an average of 48.52 fragments per primer averagely was observed. Population structure analysis anddandrogram showed distinct clustering among different wheat genotypes. Millat-11, Punjab-11, PBW-222 generated the maximum level of polymorphism, standing alone in the cluster while others are scatteredin different group.As a result, genetically numerous progenies are known, increasing the quality of sorts collections by broadening the genetic base of wheat cultivars. This study additionally indicates that RAPD markers allow quicker response and provide high throughputprocedure of accessions from a variety assortment to assess genetic diversity among wheat genotypes. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100432 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100432 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-4324-2016160282 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.59 2016 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318277689606144 |