Statistical optimization of culture conditions by response surface methodology for synthesis of lipase with Enterobacter aerogenes

Detalhes bibliográficos
Autor(a) principal: Kumari,Annapurna
Data de Publicação: 2009
Outros Autores: Mahapatra,Paramita, Banerjee,Rintu
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600005
Resumo: Optimization of lipase production by Enterobacter aerogenes was carried out using response surface methodology (RSM) where the statistical model was obtained by fractional factorial central composite design. The influence of various physico-chemical parameters, viz. temperature, oil concentration, inoculum volume, pH and incubation period on lipase production was examined. Optimization of physico-chemical parameters resulted 1.4- fold increase in lipase activity. The optimum levels of parameters were 34°C, oil concentration 3%, inoculum volume 7%, pH 7 and incubation time 60 h for obtaining a maximum lipase activity of 27.25 U/ml.
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spelling Statistical optimization of culture conditions by response surface methodology for synthesis of lipase with Enterobacter aerogenesCentral composite designEnterobacter aerogenesLipaseoptimizationparameterResponse Surface MethodologyOptimization of lipase production by Enterobacter aerogenes was carried out using response surface methodology (RSM) where the statistical model was obtained by fractional factorial central composite design. The influence of various physico-chemical parameters, viz. temperature, oil concentration, inoculum volume, pH and incubation period on lipase production was examined. Optimization of physico-chemical parameters resulted 1.4- fold increase in lipase activity. The optimum levels of parameters were 34°C, oil concentration 3%, inoculum volume 7%, pH 7 and incubation time 60 h for obtaining a maximum lipase activity of 27.25 U/ml.Instituto de Tecnologia do Paraná - Tecpar2009-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600005Brazilian Archives of Biology and Technology v.52 n.6 2009reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132009000600005info:eu-repo/semantics/openAccessKumari,AnnapurnaMahapatra,ParamitaBanerjee,Rintueng2010-02-11T00:00:00Zoai:scielo:S1516-89132009000600005Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2010-02-11T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Statistical optimization of culture conditions by response surface methodology for synthesis of lipase with Enterobacter aerogenes
title Statistical optimization of culture conditions by response surface methodology for synthesis of lipase with Enterobacter aerogenes
spellingShingle Statistical optimization of culture conditions by response surface methodology for synthesis of lipase with Enterobacter aerogenes
Kumari,Annapurna
Central composite design
Enterobacter aerogenes
Lipase
optimization
parameter
Response Surface Methodology
title_short Statistical optimization of culture conditions by response surface methodology for synthesis of lipase with Enterobacter aerogenes
title_full Statistical optimization of culture conditions by response surface methodology for synthesis of lipase with Enterobacter aerogenes
title_fullStr Statistical optimization of culture conditions by response surface methodology for synthesis of lipase with Enterobacter aerogenes
title_full_unstemmed Statistical optimization of culture conditions by response surface methodology for synthesis of lipase with Enterobacter aerogenes
title_sort Statistical optimization of culture conditions by response surface methodology for synthesis of lipase with Enterobacter aerogenes
author Kumari,Annapurna
author_facet Kumari,Annapurna
Mahapatra,Paramita
Banerjee,Rintu
author_role author
author2 Mahapatra,Paramita
Banerjee,Rintu
author2_role author
author
dc.contributor.author.fl_str_mv Kumari,Annapurna
Mahapatra,Paramita
Banerjee,Rintu
dc.subject.por.fl_str_mv Central composite design
Enterobacter aerogenes
Lipase
optimization
parameter
Response Surface Methodology
topic Central composite design
Enterobacter aerogenes
Lipase
optimization
parameter
Response Surface Methodology
description Optimization of lipase production by Enterobacter aerogenes was carried out using response surface methodology (RSM) where the statistical model was obtained by fractional factorial central composite design. The influence of various physico-chemical parameters, viz. temperature, oil concentration, inoculum volume, pH and incubation period on lipase production was examined. Optimization of physico-chemical parameters resulted 1.4- fold increase in lipase activity. The optimum levels of parameters were 34°C, oil concentration 3%, inoculum volume 7%, pH 7 and incubation time 60 h for obtaining a maximum lipase activity of 27.25 U/ml.
publishDate 2009
dc.date.none.fl_str_mv 2009-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600005
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132009000600005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.52 n.6 2009
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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