Genetic polymorphism in brazilian microcystis spp. (Cyanobacteria) toxic and non-toxic through RFLP-PCR of the cpcBA-IGS
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400014 |
Resumo: | The escalating occurrence of cyanobacterial toxic blooms demands a better understanding of genetic variability as an auxiliary expedient in species identification, collaborating with the monitoring of water destined to public supply. This study aimed at the unraveling of genetic polymorphism in the toxic and nontoxic strains of Microcystis (Cyanobacteria) species, isolated from diverse Brazilian localities through the RFLP-PCR technique applied to the c-phycocyanin encoding operon and its intergenic spacer (cpcBA-IGS). Eighteen strains belonging to M. aeruginosa, M. panniformis, M. protocystis and M. wesenbergii, plus two other unidentified strains, were analyzed by means of the morphological and molecular data. The molecular data constituted three groups with low similarity values unrelated to the geographical origin, toxicity or morphospecies. A high genetic variability among the studied populations was unveiled by the results. Brazilian populations of Microcystis spp. displayed high genetic diversity when compared to those from Australia, Japan, United States and Europe. This ample genetic diversity could be observed through the diverse eletrophoretic profiles obtained among the strains from a single species. The presence of toxic and non-toxic strains was observed in the same species, as M. aeruginosa. |
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Brazilian Archives of Biology and Technology |
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Genetic polymorphism in brazilian microcystis spp. (Cyanobacteria) toxic and non-toxic through RFLP-PCR of the cpcBA-IGSCyanobacteriacpcBA-IGSgenetic polymorphismmicrocystinMicrocystisRFLPThe escalating occurrence of cyanobacterial toxic blooms demands a better understanding of genetic variability as an auxiliary expedient in species identification, collaborating with the monitoring of water destined to public supply. This study aimed at the unraveling of genetic polymorphism in the toxic and nontoxic strains of Microcystis (Cyanobacteria) species, isolated from diverse Brazilian localities through the RFLP-PCR technique applied to the c-phycocyanin encoding operon and its intergenic spacer (cpcBA-IGS). Eighteen strains belonging to M. aeruginosa, M. panniformis, M. protocystis and M. wesenbergii, plus two other unidentified strains, were analyzed by means of the morphological and molecular data. The molecular data constituted three groups with low similarity values unrelated to the geographical origin, toxicity or morphospecies. A high genetic variability among the studied populations was unveiled by the results. Brazilian populations of Microcystis spp. displayed high genetic diversity when compared to those from Australia, Japan, United States and Europe. This ample genetic diversity could be observed through the diverse eletrophoretic profiles obtained among the strains from a single species. The presence of toxic and non-toxic strains was observed in the same species, as M. aeruginosa.Instituto de Tecnologia do Paraná - Tecpar2009-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400014Brazilian Archives of Biology and Technology v.52 n.4 2009reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132009000400014info:eu-repo/semantics/openAccessBittencourt-Oliveira,Maria do CarmoCunha,Maristela Casé CostaMoura,Ariadne do Nascimentoeng2009-09-10T00:00:00Zoai:scielo:S1516-89132009000400014Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2009-09-10T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Genetic polymorphism in brazilian microcystis spp. (Cyanobacteria) toxic and non-toxic through RFLP-PCR of the cpcBA-IGS |
title |
Genetic polymorphism in brazilian microcystis spp. (Cyanobacteria) toxic and non-toxic through RFLP-PCR of the cpcBA-IGS |
spellingShingle |
Genetic polymorphism in brazilian microcystis spp. (Cyanobacteria) toxic and non-toxic through RFLP-PCR of the cpcBA-IGS Bittencourt-Oliveira,Maria do Carmo Cyanobacteria cpcBA-IGS genetic polymorphism microcystin Microcystis RFLP |
title_short |
Genetic polymorphism in brazilian microcystis spp. (Cyanobacteria) toxic and non-toxic through RFLP-PCR of the cpcBA-IGS |
title_full |
Genetic polymorphism in brazilian microcystis spp. (Cyanobacteria) toxic and non-toxic through RFLP-PCR of the cpcBA-IGS |
title_fullStr |
Genetic polymorphism in brazilian microcystis spp. (Cyanobacteria) toxic and non-toxic through RFLP-PCR of the cpcBA-IGS |
title_full_unstemmed |
Genetic polymorphism in brazilian microcystis spp. (Cyanobacteria) toxic and non-toxic through RFLP-PCR of the cpcBA-IGS |
title_sort |
Genetic polymorphism in brazilian microcystis spp. (Cyanobacteria) toxic and non-toxic through RFLP-PCR of the cpcBA-IGS |
author |
Bittencourt-Oliveira,Maria do Carmo |
author_facet |
Bittencourt-Oliveira,Maria do Carmo Cunha,Maristela Casé Costa Moura,Ariadne do Nascimento |
author_role |
author |
author2 |
Cunha,Maristela Casé Costa Moura,Ariadne do Nascimento |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Bittencourt-Oliveira,Maria do Carmo Cunha,Maristela Casé Costa Moura,Ariadne do Nascimento |
dc.subject.por.fl_str_mv |
Cyanobacteria cpcBA-IGS genetic polymorphism microcystin Microcystis RFLP |
topic |
Cyanobacteria cpcBA-IGS genetic polymorphism microcystin Microcystis RFLP |
description |
The escalating occurrence of cyanobacterial toxic blooms demands a better understanding of genetic variability as an auxiliary expedient in species identification, collaborating with the monitoring of water destined to public supply. This study aimed at the unraveling of genetic polymorphism in the toxic and nontoxic strains of Microcystis (Cyanobacteria) species, isolated from diverse Brazilian localities through the RFLP-PCR technique applied to the c-phycocyanin encoding operon and its intergenic spacer (cpcBA-IGS). Eighteen strains belonging to M. aeruginosa, M. panniformis, M. protocystis and M. wesenbergii, plus two other unidentified strains, were analyzed by means of the morphological and molecular data. The molecular data constituted three groups with low similarity values unrelated to the geographical origin, toxicity or morphospecies. A high genetic variability among the studied populations was unveiled by the results. Brazilian populations of Microcystis spp. displayed high genetic diversity when compared to those from Australia, Japan, United States and Europe. This ample genetic diversity could be observed through the diverse eletrophoretic profiles obtained among the strains from a single species. The presence of toxic and non-toxic strains was observed in the same species, as M. aeruginosa. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400014 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400014 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132009000400014 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.52 n.4 2009 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318273141932032 |