Cytochrome P450 detection in liver of the catfish Ancistrus multispinis (Osteichthyes, Loricariidae)
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200015 |
Resumo: | Sensitive biological responses to environmental contaminants are useful as early warning signals to predict the damages by long-term exposure. Protocols standardization to quantify biochemical parameters in different fish species is required to validate its use as biomarkers. Comparative studies from different fish species and its interpretation are a challenge for the validation of its use as general biomarkers, representative of environmental impact. In this study, the protocol for liver cytochrome P450 (CYP) analysis from the native Brazilian fish Ancistrus multispinis was established. The microsome contamination by hemoglobin during the analysis of CYP in liver was detected, leading to misinterpretation of the results. The spectrophotometric method for CYP analysis was adapted in order to diminish the hemoglobin interference. Additionally, the western blotting method for CYP1A analysis was tested with success for this fish species. |
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Brazilian Archives of Biology and Technology |
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Cytochrome P450 detection in liver of the catfish Ancistrus multispinis (Osteichthyes, Loricariidae)cytochrome P450fishAncistrus multispinisbiomarkerSensitive biological responses to environmental contaminants are useful as early warning signals to predict the damages by long-term exposure. Protocols standardization to quantify biochemical parameters in different fish species is required to validate its use as biomarkers. Comparative studies from different fish species and its interpretation are a challenge for the validation of its use as general biomarkers, representative of environmental impact. In this study, the protocol for liver cytochrome P450 (CYP) analysis from the native Brazilian fish Ancistrus multispinis was established. The microsome contamination by hemoglobin during the analysis of CYP in liver was detected, leading to misinterpretation of the results. The spectrophotometric method for CYP analysis was adapted in order to diminish the hemoglobin interference. Additionally, the western blotting method for CYP1A analysis was tested with success for this fish species.Instituto de Tecnologia do Paraná - Tecpar2010-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200015Brazilian Archives of Biology and Technology v.53 n.2 2010reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132010000200015info:eu-repo/semantics/openAccessKlemz,ClaudioSalvo,Ligia MariaBastos Neto,Jayme da CunhaBainy,Afonso Celso DiasAssis,Helena Cristina da Silva deeng2010-04-06T00:00:00Zoai:scielo:S1516-89132010000200015Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2010-04-06T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Cytochrome P450 detection in liver of the catfish Ancistrus multispinis (Osteichthyes, Loricariidae) |
title |
Cytochrome P450 detection in liver of the catfish Ancistrus multispinis (Osteichthyes, Loricariidae) |
spellingShingle |
Cytochrome P450 detection in liver of the catfish Ancistrus multispinis (Osteichthyes, Loricariidae) Klemz,Claudio cytochrome P450 fish Ancistrus multispinis biomarker |
title_short |
Cytochrome P450 detection in liver of the catfish Ancistrus multispinis (Osteichthyes, Loricariidae) |
title_full |
Cytochrome P450 detection in liver of the catfish Ancistrus multispinis (Osteichthyes, Loricariidae) |
title_fullStr |
Cytochrome P450 detection in liver of the catfish Ancistrus multispinis (Osteichthyes, Loricariidae) |
title_full_unstemmed |
Cytochrome P450 detection in liver of the catfish Ancistrus multispinis (Osteichthyes, Loricariidae) |
title_sort |
Cytochrome P450 detection in liver of the catfish Ancistrus multispinis (Osteichthyes, Loricariidae) |
author |
Klemz,Claudio |
author_facet |
Klemz,Claudio Salvo,Ligia Maria Bastos Neto,Jayme da Cunha Bainy,Afonso Celso Dias Assis,Helena Cristina da Silva de |
author_role |
author |
author2 |
Salvo,Ligia Maria Bastos Neto,Jayme da Cunha Bainy,Afonso Celso Dias Assis,Helena Cristina da Silva de |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Klemz,Claudio Salvo,Ligia Maria Bastos Neto,Jayme da Cunha Bainy,Afonso Celso Dias Assis,Helena Cristina da Silva de |
dc.subject.por.fl_str_mv |
cytochrome P450 fish Ancistrus multispinis biomarker |
topic |
cytochrome P450 fish Ancistrus multispinis biomarker |
description |
Sensitive biological responses to environmental contaminants are useful as early warning signals to predict the damages by long-term exposure. Protocols standardization to quantify biochemical parameters in different fish species is required to validate its use as biomarkers. Comparative studies from different fish species and its interpretation are a challenge for the validation of its use as general biomarkers, representative of environmental impact. In this study, the protocol for liver cytochrome P450 (CYP) analysis from the native Brazilian fish Ancistrus multispinis was established. The microsome contamination by hemoglobin during the analysis of CYP in liver was detected, leading to misinterpretation of the results. The spectrophotometric method for CYP analysis was adapted in order to diminish the hemoglobin interference. Additionally, the western blotting method for CYP1A analysis was tested with success for this fish species. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200015 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200015 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132010000200015 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.53 n.2 2010 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318273658880000 |