Cytochrome P450 detection in liver of the catfish Ancistrus multispinis (Osteichthyes, Loricariidae)

Detalhes bibliográficos
Autor(a) principal: Klemz,Claudio
Data de Publicação: 2010
Outros Autores: Salvo,Ligia Maria, Bastos Neto,Jayme da Cunha, Bainy,Afonso Celso Dias, Assis,Helena Cristina da Silva de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200015
Resumo: Sensitive biological responses to environmental contaminants are useful as early warning signals to predict the damages by long-term exposure. Protocols standardization to quantify biochemical parameters in different fish species is required to validate its use as biomarkers. Comparative studies from different fish species and its interpretation are a challenge for the validation of its use as general biomarkers, representative of environmental impact. In this study, the protocol for liver cytochrome P450 (CYP) analysis from the native Brazilian fish Ancistrus multispinis was established. The microsome contamination by hemoglobin during the analysis of CYP in liver was detected, leading to misinterpretation of the results. The spectrophotometric method for CYP analysis was adapted in order to diminish the hemoglobin interference. Additionally, the western blotting method for CYP1A analysis was tested with success for this fish species.
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spelling Cytochrome P450 detection in liver of the catfish Ancistrus multispinis (Osteichthyes, Loricariidae)cytochrome P450fishAncistrus multispinisbiomarkerSensitive biological responses to environmental contaminants are useful as early warning signals to predict the damages by long-term exposure. Protocols standardization to quantify biochemical parameters in different fish species is required to validate its use as biomarkers. Comparative studies from different fish species and its interpretation are a challenge for the validation of its use as general biomarkers, representative of environmental impact. In this study, the protocol for liver cytochrome P450 (CYP) analysis from the native Brazilian fish Ancistrus multispinis was established. The microsome contamination by hemoglobin during the analysis of CYP in liver was detected, leading to misinterpretation of the results. The spectrophotometric method for CYP analysis was adapted in order to diminish the hemoglobin interference. Additionally, the western blotting method for CYP1A analysis was tested with success for this fish species.Instituto de Tecnologia do Paraná - Tecpar2010-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200015Brazilian Archives of Biology and Technology v.53 n.2 2010reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132010000200015info:eu-repo/semantics/openAccessKlemz,ClaudioSalvo,Ligia MariaBastos Neto,Jayme da CunhaBainy,Afonso Celso DiasAssis,Helena Cristina da Silva deeng2010-04-06T00:00:00Zoai:scielo:S1516-89132010000200015Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2010-04-06T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Cytochrome P450 detection in liver of the catfish Ancistrus multispinis (Osteichthyes, Loricariidae)
title Cytochrome P450 detection in liver of the catfish Ancistrus multispinis (Osteichthyes, Loricariidae)
spellingShingle Cytochrome P450 detection in liver of the catfish Ancistrus multispinis (Osteichthyes, Loricariidae)
Klemz,Claudio
cytochrome P450
fish
Ancistrus multispinis
biomarker
title_short Cytochrome P450 detection in liver of the catfish Ancistrus multispinis (Osteichthyes, Loricariidae)
title_full Cytochrome P450 detection in liver of the catfish Ancistrus multispinis (Osteichthyes, Loricariidae)
title_fullStr Cytochrome P450 detection in liver of the catfish Ancistrus multispinis (Osteichthyes, Loricariidae)
title_full_unstemmed Cytochrome P450 detection in liver of the catfish Ancistrus multispinis (Osteichthyes, Loricariidae)
title_sort Cytochrome P450 detection in liver of the catfish Ancistrus multispinis (Osteichthyes, Loricariidae)
author Klemz,Claudio
author_facet Klemz,Claudio
Salvo,Ligia Maria
Bastos Neto,Jayme da Cunha
Bainy,Afonso Celso Dias
Assis,Helena Cristina da Silva de
author_role author
author2 Salvo,Ligia Maria
Bastos Neto,Jayme da Cunha
Bainy,Afonso Celso Dias
Assis,Helena Cristina da Silva de
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Klemz,Claudio
Salvo,Ligia Maria
Bastos Neto,Jayme da Cunha
Bainy,Afonso Celso Dias
Assis,Helena Cristina da Silva de
dc.subject.por.fl_str_mv cytochrome P450
fish
Ancistrus multispinis
biomarker
topic cytochrome P450
fish
Ancistrus multispinis
biomarker
description Sensitive biological responses to environmental contaminants are useful as early warning signals to predict the damages by long-term exposure. Protocols standardization to quantify biochemical parameters in different fish species is required to validate its use as biomarkers. Comparative studies from different fish species and its interpretation are a challenge for the validation of its use as general biomarkers, representative of environmental impact. In this study, the protocol for liver cytochrome P450 (CYP) analysis from the native Brazilian fish Ancistrus multispinis was established. The microsome contamination by hemoglobin during the analysis of CYP in liver was detected, leading to misinterpretation of the results. The spectrophotometric method for CYP analysis was adapted in order to diminish the hemoglobin interference. Additionally, the western blotting method for CYP1A analysis was tested with success for this fish species.
publishDate 2010
dc.date.none.fl_str_mv 2010-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200015
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200015
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132010000200015
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.53 n.2 2010
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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