Azadirachtin biosynthesis induction in Azadirachta indica A. Juss cotyledonary calli with elicitor agents
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200001 |
Resumo: | The use of cell and plant tissues culture techniques to produce economically important active metabolites has been growing. Among these substances, azadirachtin (AZA), produced by the neem tree (Azadirachta indica), has received considerable attention due to its bioinsecticide action. The main goal of this work was to analyze the AZA levels in neem cotyledonary calli. The calli were grown in agitated Woody Plant Medium (WPM) liquid medium, supplemented with glucose (Gl), hydrolyzed casein (HC) and methyl jasmonate (MeJ) as elicitor agent. An interaction was observed between these substances, depending on in vitro cultivation time with orbital agitation. The highest concentrations (average of 0.2470 µg g-1) of AZA were produced in the first and second weeks of culture when the cell mass was grown in a medium with 2% Gl v/v, 500 mg L-1 HC and 100 µM of MeJ. This corresponded to approximately 57% of the AZA content stored in the donor plants seeds, used as a source of explants to induce in vitro callus formation. It was concluded that the nutrition, as well as the concentration of MeJ as signal transduction of secondary metabolism in neem cells, might influence the AZA content produced in vitro. |
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Brazilian Archives of Biology and Technology |
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Azadirachtin biosynthesis induction in Azadirachta indica A. Juss cotyledonary calli with elicitor agentsNeemHPLCglucosehydrolysed caseinmethyl jasmonateThe use of cell and plant tissues culture techniques to produce economically important active metabolites has been growing. Among these substances, azadirachtin (AZA), produced by the neem tree (Azadirachta indica), has received considerable attention due to its bioinsecticide action. The main goal of this work was to analyze the AZA levels in neem cotyledonary calli. The calli were grown in agitated Woody Plant Medium (WPM) liquid medium, supplemented with glucose (Gl), hydrolyzed casein (HC) and methyl jasmonate (MeJ) as elicitor agent. An interaction was observed between these substances, depending on in vitro cultivation time with orbital agitation. The highest concentrations (average of 0.2470 µg g-1) of AZA were produced in the first and second weeks of culture when the cell mass was grown in a medium with 2% Gl v/v, 500 mg L-1 HC and 100 µM of MeJ. This corresponded to approximately 57% of the AZA content stored in the donor plants seeds, used as a source of explants to induce in vitro callus formation. It was concluded that the nutrition, as well as the concentration of MeJ as signal transduction of secondary metabolism in neem cells, might influence the AZA content produced in vitro.Instituto de Tecnologia do Paraná - Tecpar2014-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200001Brazilian Archives of Biology and Technology v.57 n.2 2014reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132014000200001info:eu-repo/semantics/openAccessRodrigues,MarceloFestucci-Buselli,Reginaldo AlvesSilva,Luzimar CamposOtoni,Wagner Camposeng2014-04-04T00:00:00Zoai:scielo:S1516-89132014000200001Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2014-04-04T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Azadirachtin biosynthesis induction in Azadirachta indica A. Juss cotyledonary calli with elicitor agents |
title |
Azadirachtin biosynthesis induction in Azadirachta indica A. Juss cotyledonary calli with elicitor agents |
spellingShingle |
Azadirachtin biosynthesis induction in Azadirachta indica A. Juss cotyledonary calli with elicitor agents Rodrigues,Marcelo Neem HPLC glucose hydrolysed casein methyl jasmonate |
title_short |
Azadirachtin biosynthesis induction in Azadirachta indica A. Juss cotyledonary calli with elicitor agents |
title_full |
Azadirachtin biosynthesis induction in Azadirachta indica A. Juss cotyledonary calli with elicitor agents |
title_fullStr |
Azadirachtin biosynthesis induction in Azadirachta indica A. Juss cotyledonary calli with elicitor agents |
title_full_unstemmed |
Azadirachtin biosynthesis induction in Azadirachta indica A. Juss cotyledonary calli with elicitor agents |
title_sort |
Azadirachtin biosynthesis induction in Azadirachta indica A. Juss cotyledonary calli with elicitor agents |
author |
Rodrigues,Marcelo |
author_facet |
Rodrigues,Marcelo Festucci-Buselli,Reginaldo Alves Silva,Luzimar Campos Otoni,Wagner Campos |
author_role |
author |
author2 |
Festucci-Buselli,Reginaldo Alves Silva,Luzimar Campos Otoni,Wagner Campos |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Rodrigues,Marcelo Festucci-Buselli,Reginaldo Alves Silva,Luzimar Campos Otoni,Wagner Campos |
dc.subject.por.fl_str_mv |
Neem HPLC glucose hydrolysed casein methyl jasmonate |
topic |
Neem HPLC glucose hydrolysed casein methyl jasmonate |
description |
The use of cell and plant tissues culture techniques to produce economically important active metabolites has been growing. Among these substances, azadirachtin (AZA), produced by the neem tree (Azadirachta indica), has received considerable attention due to its bioinsecticide action. The main goal of this work was to analyze the AZA levels in neem cotyledonary calli. The calli were grown in agitated Woody Plant Medium (WPM) liquid medium, supplemented with glucose (Gl), hydrolyzed casein (HC) and methyl jasmonate (MeJ) as elicitor agent. An interaction was observed between these substances, depending on in vitro cultivation time with orbital agitation. The highest concentrations (average of 0.2470 µg g-1) of AZA were produced in the first and second weeks of culture when the cell mass was grown in a medium with 2% Gl v/v, 500 mg L-1 HC and 100 µM of MeJ. This corresponded to approximately 57% of the AZA content stored in the donor plants seeds, used as a source of explants to induce in vitro callus formation. It was concluded that the nutrition, as well as the concentration of MeJ as signal transduction of secondary metabolism in neem cells, might influence the AZA content produced in vitro. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200001 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132014000200001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.57 n.2 2014 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318276076896256 |