Somatic Embryogenesis in Parana Pine (Araucaria angustifolia (Bert.) O. Kuntze)
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000100015 |
Resumo: | Embryogenic cultures of Araucaria angustifolia were induced from dominant and non-dominant zygotic embryos excised from immature seeds proceeding from three different genotypes and five harvest dates. Zygotic embryos were inoculated in inductive culture medium LP and BM supplemented with or without plant growth regulators 2,4-D (5 µM), BA (2 µM) and Kin (2 µM). The genotype of the mother tree and the developmental explant stage affected the induction frequency. In the maintenance phase, embryogenic cultures were maintained at continuous repetitive cell cycles every 20 days in semi-solid or liquid medium. In the maturation phase the culture medium was supplemented with different types and levels of growth regulators, osmotic agents, carbohydrates and derived. Embryogenic cultures inoculated in culture medium supplemented with PEG 3350 (6 and 9%), maltose (6 and 9%), plus BA and Kin (1 µM each) resulted in the progression of somatic embryos to globular and torpedo developmental stages. |
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Somatic Embryogenesis in Parana Pine (Araucaria angustifolia (Bert.) O. Kuntze)Araucaria angustifoliaconifersomatic embryogenesisPicea abiesembryo maturationPinus sp.Embryogenic cultures of Araucaria angustifolia were induced from dominant and non-dominant zygotic embryos excised from immature seeds proceeding from three different genotypes and five harvest dates. Zygotic embryos were inoculated in inductive culture medium LP and BM supplemented with or without plant growth regulators 2,4-D (5 µM), BA (2 µM) and Kin (2 µM). The genotype of the mother tree and the developmental explant stage affected the induction frequency. In the maintenance phase, embryogenic cultures were maintained at continuous repetitive cell cycles every 20 days in semi-solid or liquid medium. In the maturation phase the culture medium was supplemented with different types and levels of growth regulators, osmotic agents, carbohydrates and derived. Embryogenic cultures inoculated in culture medium supplemented with PEG 3350 (6 and 9%), maltose (6 and 9%), plus BA and Kin (1 µM each) resulted in the progression of somatic embryos to globular and torpedo developmental stages.Instituto de Tecnologia do Paraná - Tecpar2002-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000100015Brazilian Archives of Biology and Technology v.45 n.1 2002reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132002000100015info:eu-repo/semantics/openAccessSantos,André Luis Wendt dosSilveira,VanildoSteiner,NeusaVidor,MárioGuerra,Miguel Pedroeng2002-10-07T00:00:00Zoai:scielo:S1516-89132002000100015Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2002-10-07T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Somatic Embryogenesis in Parana Pine (Araucaria angustifolia (Bert.) O. Kuntze) |
title |
Somatic Embryogenesis in Parana Pine (Araucaria angustifolia (Bert.) O. Kuntze) |
spellingShingle |
Somatic Embryogenesis in Parana Pine (Araucaria angustifolia (Bert.) O. Kuntze) Santos,André Luis Wendt dos Araucaria angustifolia conifer somatic embryogenesis Picea abies embryo maturation Pinus sp. |
title_short |
Somatic Embryogenesis in Parana Pine (Araucaria angustifolia (Bert.) O. Kuntze) |
title_full |
Somatic Embryogenesis in Parana Pine (Araucaria angustifolia (Bert.) O. Kuntze) |
title_fullStr |
Somatic Embryogenesis in Parana Pine (Araucaria angustifolia (Bert.) O. Kuntze) |
title_full_unstemmed |
Somatic Embryogenesis in Parana Pine (Araucaria angustifolia (Bert.) O. Kuntze) |
title_sort |
Somatic Embryogenesis in Parana Pine (Araucaria angustifolia (Bert.) O. Kuntze) |
author |
Santos,André Luis Wendt dos |
author_facet |
Santos,André Luis Wendt dos Silveira,Vanildo Steiner,Neusa Vidor,Mário Guerra,Miguel Pedro |
author_role |
author |
author2 |
Silveira,Vanildo Steiner,Neusa Vidor,Mário Guerra,Miguel Pedro |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Santos,André Luis Wendt dos Silveira,Vanildo Steiner,Neusa Vidor,Mário Guerra,Miguel Pedro |
dc.subject.por.fl_str_mv |
Araucaria angustifolia conifer somatic embryogenesis Picea abies embryo maturation Pinus sp. |
topic |
Araucaria angustifolia conifer somatic embryogenesis Picea abies embryo maturation Pinus sp. |
description |
Embryogenic cultures of Araucaria angustifolia were induced from dominant and non-dominant zygotic embryos excised from immature seeds proceeding from three different genotypes and five harvest dates. Zygotic embryos were inoculated in inductive culture medium LP and BM supplemented with or without plant growth regulators 2,4-D (5 µM), BA (2 µM) and Kin (2 µM). The genotype of the mother tree and the developmental explant stage affected the induction frequency. In the maintenance phase, embryogenic cultures were maintained at continuous repetitive cell cycles every 20 days in semi-solid or liquid medium. In the maturation phase the culture medium was supplemented with different types and levels of growth regulators, osmotic agents, carbohydrates and derived. Embryogenic cultures inoculated in culture medium supplemented with PEG 3350 (6 and 9%), maltose (6 and 9%), plus BA and Kin (1 µM each) resulted in the progression of somatic embryos to globular and torpedo developmental stages. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000100015 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000100015 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132002000100015 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.45 n.1 2002 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318268748398592 |