Bauhinia forficata link shoot regeneration: histological analysis of organogenesis pathway

Detalhes bibliográficos
Autor(a) principal: Mello,Marcia O.
Data de Publicação: 2000
Outros Autores: Melo,Murilo, Appezzato-da-Glória,Beatriz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400013
Resumo: Plant regeneration was achieved from cells of callus induced from hypocotyl segments of Bauhinia forficata on half strength Murashige and Skoog culture medium supplemented with several concentrations of BAP. Within 40 days of culture shoot buds formation was observed on callus surface. Calli were then transferred to a same composition culture medium without plant growth regulator in order to induce shoot elongation. Histological studies indicated that in vitro plant regeneration in B. forficata occurred through indirect organogenesis. Meristemoids consisting of small cells with dense cytoplasm and prominent nuclei were randomly distributed throughout the callus surface indicating early stages of shoot bud differentiation. Shoots developed de novo from superficial layers of cells and the pattern of shoot origin and development were very similar to those previously described for other leguminous species.
id TECPAR-1_ebe4d6468fed7932a7997b05efdf7847
oai_identifier_str oai:scielo:S1516-89132000000400013
network_acronym_str TECPAR-1
network_name_str Brazilian Archives of Biology and Technology
repository_id_str
spelling Bauhinia forficata link shoot regeneration: histological analysis of organogenesis pathwayTissue cultureorganogenesisanatomyPlant regeneration was achieved from cells of callus induced from hypocotyl segments of Bauhinia forficata on half strength Murashige and Skoog culture medium supplemented with several concentrations of BAP. Within 40 days of culture shoot buds formation was observed on callus surface. Calli were then transferred to a same composition culture medium without plant growth regulator in order to induce shoot elongation. Histological studies indicated that in vitro plant regeneration in B. forficata occurred through indirect organogenesis. Meristemoids consisting of small cells with dense cytoplasm and prominent nuclei were randomly distributed throughout the callus surface indicating early stages of shoot bud differentiation. Shoots developed de novo from superficial layers of cells and the pattern of shoot origin and development were very similar to those previously described for other leguminous species.Instituto de Tecnologia do Paraná - Tecpar2000-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400013Brazilian Archives of Biology and Technology v.43 n.4 2000reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132000000400013info:eu-repo/semantics/openAccessMello,Marcia O.Melo,MuriloAppezzato-da-Glória,Beatrizeng2007-03-12T00:00:00Zoai:scielo:S1516-89132000000400013Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2007-03-12T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Bauhinia forficata link shoot regeneration: histological analysis of organogenesis pathway
title Bauhinia forficata link shoot regeneration: histological analysis of organogenesis pathway
spellingShingle Bauhinia forficata link shoot regeneration: histological analysis of organogenesis pathway
Mello,Marcia O.
Tissue culture
organogenesis
anatomy
title_short Bauhinia forficata link shoot regeneration: histological analysis of organogenesis pathway
title_full Bauhinia forficata link shoot regeneration: histological analysis of organogenesis pathway
title_fullStr Bauhinia forficata link shoot regeneration: histological analysis of organogenesis pathway
title_full_unstemmed Bauhinia forficata link shoot regeneration: histological analysis of organogenesis pathway
title_sort Bauhinia forficata link shoot regeneration: histological analysis of organogenesis pathway
author Mello,Marcia O.
author_facet Mello,Marcia O.
Melo,Murilo
Appezzato-da-Glória,Beatriz
author_role author
author2 Melo,Murilo
Appezzato-da-Glória,Beatriz
author2_role author
author
dc.contributor.author.fl_str_mv Mello,Marcia O.
Melo,Murilo
Appezzato-da-Glória,Beatriz
dc.subject.por.fl_str_mv Tissue culture
organogenesis
anatomy
topic Tissue culture
organogenesis
anatomy
description Plant regeneration was achieved from cells of callus induced from hypocotyl segments of Bauhinia forficata on half strength Murashige and Skoog culture medium supplemented with several concentrations of BAP. Within 40 days of culture shoot buds formation was observed on callus surface. Calli were then transferred to a same composition culture medium without plant growth regulator in order to induce shoot elongation. Histological studies indicated that in vitro plant regeneration in B. forficata occurred through indirect organogenesis. Meristemoids consisting of small cells with dense cytoplasm and prominent nuclei were randomly distributed throughout the callus surface indicating early stages of shoot bud differentiation. Shoots developed de novo from superficial layers of cells and the pattern of shoot origin and development were very similar to those previously described for other leguminous species.
publishDate 2000
dc.date.none.fl_str_mv 2000-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400013
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132000000400013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.43 n.4 2000
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
_version_ 1750318268325822464

Registros relacionados