Blood glucose regulation during fasting in rats under food restriction since birth

• Autor(a) principal: Vitoriano,Adriana de Souza
• Data de Publicação: 2011
• Outros Autores: Malta,Ananda, Barrena,Helenton Christian, Furlan,Maria Montserrat Diaz Pedrosa, Bazotte,Roberto Barbosa, Gazola,Vilma Godoi
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Archives of Biology and Technology
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100009
• topic: food restriction; rats; liver; gluconeogenesis; glycogenolysis

The effect of severe food restriction since birth on regulation of fasting glycemia in male Wistar rats was investigated. The control group (CG) had free supply of chow, while the restriction group (RG) received 50% of the amount ingested by the CG. The experiments were done in adult (60 days) overnight fasted rats in which glycemia, liver free glucose levels and hepatic glycogen concentration were measured. In part of the experiments in situ liver perfusion was done. The results showed that livers from the RG had higher glycogenolysis rates but lower gluconeogenesis rates from L-alanine (10 mM). Since RG showed maintained glycemia during fasting, it could be concluded that livers from RG produced glucose preferentially from glycogenolysis in detriment of gluconeogenesis. These findings demonstrated that in spite of severe caloric restriction, the metabolic adaptations of the liver did exist to assure the maintenance of blood glucose for brain supply during fasting.