Genetic analysis of species in the Genus Catasetum (ORCHIDACEAE) using RAPD Markers

• Autor(a) principal: Oliveira,Luciana do Valle Rego
• Data de Publicação: 2010
• Outros Autores: Faria,Ricardo Tadeu de, Ruas,Claudete de Fátima, Ruas,Paulo Maurício, Santos,Melissa de Oliveira, Carvalho,Valdemar P.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Archives of Biology and Technology
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200017
• topic: Catasetum; molecular marker; orchid; RAPD; genetic similarity

In this work, RAPD molecular markers were used to access the genetic variability and to study the inter and intraespecifc relationship in a group of 37 species, including 56 individuals. A total of 15 RAPD primers were selected for DNA amplification. From a total of 221 bands analyzed, 209 (95%) were polymorphics. The level of interespecifc genetic similarity ranged from 37% between Catasetum complanatum and Catasetum laminatum to 83% between Catasetum triodon and Catasetum uncatum. The intraspecifc genetic similarity varied 88% for the individuals of Catasetum triodon to 93% between the individuals of Catasetum atratum and Catasetum macrocarpum. These results would contribute to understand the genetic relationship in Catasetum, to define the strategies to establish a germplasm core collection for the genus and to provide support for breeding programs.