Physiological Responses of Callus from Gerbera jamesonii Bolus ex. Hook f. to Gamma Irradiation

Detalhes bibliográficos
Autor(a) principal: Hasbullah,Nor Azlina
Data de Publicação: 2012
Outros Autores: Taha,Rosna Mat, Saleh,Azani, Mohamed,Normadiha
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300012
Resumo: In the present study, in vitro mutagenesis techniques were applied to investigate the effects of gamma irradiation at 0, 10, 20, 30, 40, 50 and 60 Gy on physiological changes in callus of Gerbera jamesonii Bolus ex. Hook f. Biochemical changes in chlorophyll and soluble protein content of pre- and post- irradiated Gerbera callus were studied. Non-irradiated callus demonstrated the highest amount of chlorophyll content as compared to callus irradiated at 10, 20, 30, 40, 50 and 60 Gy. In addition, the amount of chlorophyll b was relatively higher than chlorophyll a in both the irradiated and non-irradiated callus, except for callus irradiated at 10 Gy. Biochemical differentiation based on total soluble protein content revealed gradual reduction after day 9 of exposure to gamma irradiation. Reduction of soluble protein content was observed in all the treatments as the increase of incubation period.
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spelling Physiological Responses of Callus from Gerbera jamesonii Bolus ex. Hook f. to Gamma IrradiationGerbera jamesoniigamma irradiationcallusin vitrochlorophyllsoluble proteinIn the present study, in vitro mutagenesis techniques were applied to investigate the effects of gamma irradiation at 0, 10, 20, 30, 40, 50 and 60 Gy on physiological changes in callus of Gerbera jamesonii Bolus ex. Hook f. Biochemical changes in chlorophyll and soluble protein content of pre- and post- irradiated Gerbera callus were studied. Non-irradiated callus demonstrated the highest amount of chlorophyll content as compared to callus irradiated at 10, 20, 30, 40, 50 and 60 Gy. In addition, the amount of chlorophyll b was relatively higher than chlorophyll a in both the irradiated and non-irradiated callus, except for callus irradiated at 10 Gy. Biochemical differentiation based on total soluble protein content revealed gradual reduction after day 9 of exposure to gamma irradiation. Reduction of soluble protein content was observed in all the treatments as the increase of incubation period.Instituto de Tecnologia do Paraná - Tecpar2012-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300012Brazilian Archives of Biology and Technology v.55 n.3 2012reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132012000300012info:eu-repo/semantics/openAccessHasbullah,Nor AzlinaTaha,Rosna MatSaleh,AzaniMohamed,Normadihaeng2012-07-03T00:00:00Zoai:scielo:S1516-89132012000300012Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2012-07-03T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Physiological Responses of Callus from Gerbera jamesonii Bolus ex. Hook f. to Gamma Irradiation
title Physiological Responses of Callus from Gerbera jamesonii Bolus ex. Hook f. to Gamma Irradiation
spellingShingle Physiological Responses of Callus from Gerbera jamesonii Bolus ex. Hook f. to Gamma Irradiation
Hasbullah,Nor Azlina
Gerbera jamesonii
gamma irradiation
callus
in vitro
chlorophyll
soluble protein
title_short Physiological Responses of Callus from Gerbera jamesonii Bolus ex. Hook f. to Gamma Irradiation
title_full Physiological Responses of Callus from Gerbera jamesonii Bolus ex. Hook f. to Gamma Irradiation
title_fullStr Physiological Responses of Callus from Gerbera jamesonii Bolus ex. Hook f. to Gamma Irradiation
title_full_unstemmed Physiological Responses of Callus from Gerbera jamesonii Bolus ex. Hook f. to Gamma Irradiation
title_sort Physiological Responses of Callus from Gerbera jamesonii Bolus ex. Hook f. to Gamma Irradiation
author Hasbullah,Nor Azlina
author_facet Hasbullah,Nor Azlina
Taha,Rosna Mat
Saleh,Azani
Mohamed,Normadiha
author_role author
author2 Taha,Rosna Mat
Saleh,Azani
Mohamed,Normadiha
author2_role author
author
author
dc.contributor.author.fl_str_mv Hasbullah,Nor Azlina
Taha,Rosna Mat
Saleh,Azani
Mohamed,Normadiha
dc.subject.por.fl_str_mv Gerbera jamesonii
gamma irradiation
callus
in vitro
chlorophyll
soluble protein
topic Gerbera jamesonii
gamma irradiation
callus
in vitro
chlorophyll
soluble protein
description In the present study, in vitro mutagenesis techniques were applied to investigate the effects of gamma irradiation at 0, 10, 20, 30, 40, 50 and 60 Gy on physiological changes in callus of Gerbera jamesonii Bolus ex. Hook f. Biochemical changes in chlorophyll and soluble protein content of pre- and post- irradiated Gerbera callus were studied. Non-irradiated callus demonstrated the highest amount of chlorophyll content as compared to callus irradiated at 10, 20, 30, 40, 50 and 60 Gy. In addition, the amount of chlorophyll b was relatively higher than chlorophyll a in both the irradiated and non-irradiated callus, except for callus irradiated at 10 Gy. Biochemical differentiation based on total soluble protein content revealed gradual reduction after day 9 of exposure to gamma irradiation. Reduction of soluble protein content was observed in all the treatments as the increase of incubation period.
publishDate 2012
dc.date.none.fl_str_mv 2012-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300012
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132012000300012
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.55 n.3 2012
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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