Fungos filamentosos do lodo de esgoto: impacto na microbiota fúngica e potencial enzimático

• Autor(a) principal: Leite, Marcela Vieira
• Data de Publicação: 2009
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UNICAP
• Texto Completo: http://tede2.unicap.br:8080/handle/tede/590
• topic: lodo de esgoto; enzimas; mofo (botânica); dissertação; sewage sludge, enzymes, mold (botany); dissertation; CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL

The Stations Wastewater Treatment Plant appeared to lessen the pollution in water flows resulting from dumping of waste, such as sewage sludge. This residue is widely used in agriculture to be rich in organic matter and nutrients, helping in the productivity and soil fertility and contributing to the increase in the microbiota. In this work, we characterized the fungi living in the sewage sludge, soil and soil with the addition of sludge, assessing the potential of biotechnology mycobiota the mud with a brief study on the effect of temperature on enzyme activity of the same. The sewage sludge was collected at the Wastewater Treatment Plant Mangueira, Recife-PE and soil collected at the Experimental Station of Itapirema-IPA, city of Goiana-PE. The additions of sewage sludge to the soil were made at doses of 0, 25, 50 and 75 ton/ha. For the isolation of fungal microbiota was the technique of serial dilution and subsequent plating on selective media. The identification of mycobiota was made through the analysis of macroscopic and microscopic characteristics of the isolates, based on the work described by other authors. The detection of enzyme activity of cellulase, lipase and urease was based on the methodology described by Hankin and Anagnostakis (1975), with analysis of the influence of temperature on activities. The results show that the sewage sludge are present the genera Aspergillus sp., Chrysosporium sp., Monotospora sp., Penicillium sp. and Scedosporium sp.; soil the genera Aspergillus sp., Cladosporium sp., Mucor sp. and Penicillium sp. and soil with the addition of sewage sludge the genera Aspergillus sp. and Penicillium sp., showing the impact on mycobiota soil. Fungi the sludge showed better results in the lipase activity, with the genus Chrysosporium sp. presented as the best enzyme activity in cellulase and lipase in the two tested temperatures (28°C and 35°C). The urease activity was shown with little significant value in relation to the results of other enzymes