An international parentage and identification panel for the domestic cat (Felis catus)

• Autor(a) principal: Lipinski, Monika J.
• Data de Publicação: 2007
• Outros Autores: Amigues, Yves, Blasi, Michele, Broad, Thomas E., Cherbonnel, Corinne, Cho, Gil Jae, Corley, Sean, Daftari, Pratibha, Delattre, Delphine R., Dileanis, Susan G., Flynn, John M., Grattapaglia, Dario, Guthrie, Alan, Harper, Cindy, Karttunen, Pirjo L., Kimura, Hirohisa, Lewis, Gavin M., Longeri, Maria, Meriaux, Jean Claude, Morita, Mitsuo, Morrin-O'Donnell, Romy C., Niini, Tirri, Pedersen, Niels C., Perrotta, Giovanna, Polli, Michele, Rittler, Stephanie, Schubbert, Rainer, Strillacci, Maria Giuseppina, Haeringen, Hein Van, Haeringen, Wim A. Van, Lyons, Leslie A.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UCB
• Texto Completo: http://twingo.ucb.br:8080/jspui/handle/10869/678; https://repositorio.ucb.br:9443/jspui/handle/123456789/7820
• topic: Cat; Feline; Identification; Microsatellite; Parentage

Seventeen commercial and research laboratories participated in two comparison tests under the auspices of the International Society for Animal Genetics to develop an internationally tested, microsatellite-based parentage and identification panel for the domestic cat (Felis catus). Genetic marker selection was based on the polymorphism information content and allele ranges from seven random-bred populations (n ¼ 261) from the USA, Europe and Brazil and eight breeds (n ¼ 200) from the USA. Nineteen microsatellite markers were included in the comparison test and genotyped across the samples. Based on robustness and efficiency, nine autosomal microsatellite markers were ultimately selected as a single multiplex core panel for cat identification and parentage testing. Most markers contained dinucleotide repeats. In addition to the autosomal markers, the panel included two genderspecific markers, amelogenin and zinc-finger XY, which produced genotypes for both the X and Y chromosomes. This international cat parentage and identification panel has a power of exclusion comparable to panels used in other species, ranging from 90.08% to 99.79% across breeds and 99.47% to 99.87% in random-bred cat populations.