Development and characterization of microsatellite markers for genetic analysis

• Autor(a) principal: Collevatt, Rosane Garcia
• Data de Publicação: 1999
• Outros Autores: Brondani, Rosana Vianello, Grattapaglia, Dario
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UCB
• Texto Completo: http://twingo.ucb.br:8080/jspui/handle/10869/368; https://repositorio.ucb.br:9443/jspui/handle/123456789/7517
• topic: Caryocar brasiliense; Caryocaraceae; Cerrado; Microsatellites; Tropical tree

In this work we report the development and characterization of 10 microsatellite loci for the endangered tree species Caryocar brasiliense. Using genomic library enrichment, the e ciency of SS marker development was 14.4% from sequencing data to operationally useful loci. Primer sequences for this set of 10 loci are made available together with their estimates of expected eterozygosity, probability of paternity exclusion and probability of identity. Mendelian heritance and segregation was con®rmed for all 10 loci in open-pollinated half-sib families as well as the absolute transferability of these 10 loci to ®ve other species of the same genus. Number of alleles per locus ranged from 10 to 22 with a mean value of 16 and expected heterozygosity varying from 0.84 to 0.94. The combined probability of genetic identity was on the order of 10±17 clearly demonstrating that SSR multilocus genotypes are likely to be unique and capable of readily discriminating individuals of C. brasiliense. The very high combined probability of paternity exclusion (0.99999995) also indicates that these markers will permit detailed parentage studies in natural populations even in situations where both maternity and paternity are unknown. The battery of microsatellite markers developed and characterized in this study opens a new perspective for the generation of fundamental population genetic data for devising sound collection and conservation procedures for C. brasiliense and related species of the genus.