Trichomonas vaginalis: IdentiWcation of a triacylglycerol acylhydrolase

Detalhes bibliográficos
Autor(a) principal: Carvalho, Sandra Elisa
Data de Publicação: 2005
Outros Autores: Freitas, Daniel, Murad, André Melro, Franco, Octávio Luiz, Simões-Barbosa, Augusto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UCB
Texto Completo: http://twingo.ucb.br:8080/jspui/handle/10869/589
https://repositorio.ucb.br:9443/jspui/handle/123456789/7831
Resumo: This work describes the identiWcation of a triacylglycerol lipase named TVLip directly onto blood-LB-agar plates by hemolytic screening of a Trichomonas vaginalis cDNA expression library. Sharing signiWcant similarity in the primary sequence with other lipases, the theoretical 3D structure of the TVLip was resolved. The structure reveals the predictive conserved characteristics of other lipases from EC3.1.1.3 group, although presenting one aminoacid change in the catalytic triad Ser-His-Asp. Finally, analysis of Northern blot indicates that the expression of the TVLip gene is up-regulated by iron.
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spelling Carvalho, Sandra ElisaFreitas, DanielMurad, André MelroFranco, Octávio LuizSimões-Barbosa, Augusto2016-10-10T03:52:49Z2016-10-10T03:52:49Z2005CARVALHO, Sandra Elisa et al. Trichomonas vaginalis: Identification of a triacylglycerol acylhydrolase . Experimental Parasitology, v. 111, p. 260-263, 2005.00144894http://twingo.ucb.br:8080/jspui/handle/10869/589https://repositorio.ucb.br:9443/jspui/handle/123456789/7831This work describes the identiWcation of a triacylglycerol lipase named TVLip directly onto blood-LB-agar plates by hemolytic screening of a Trichomonas vaginalis cDNA expression library. Sharing signiWcant similarity in the primary sequence with other lipases, the theoretical 3D structure of the TVLip was resolved. The structure reveals the predictive conserved characteristics of other lipases from EC3.1.1.3 group, although presenting one aminoacid change in the catalytic triad Ser-His-Asp. Finally, analysis of Northern blot indicates that the expression of the TVLip gene is up-regulated by iron.Made available in DSpace on 2016-10-10T03:52:49Z (GMT). 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dc.title.pt_BR.fl_str_mv Trichomonas vaginalis: IdentiWcation of a triacylglycerol acylhydrolase
title Trichomonas vaginalis: IdentiWcation of a triacylglycerol acylhydrolase
spellingShingle Trichomonas vaginalis: IdentiWcation of a triacylglycerol acylhydrolase
Carvalho, Sandra Elisa
Trichomonas vaginallis
Lipase
Molecular modelling
Hemolysis
Pathogenesis
title_short Trichomonas vaginalis: IdentiWcation of a triacylglycerol acylhydrolase
title_full Trichomonas vaginalis: IdentiWcation of a triacylglycerol acylhydrolase
title_fullStr Trichomonas vaginalis: IdentiWcation of a triacylglycerol acylhydrolase
title_full_unstemmed Trichomonas vaginalis: IdentiWcation of a triacylglycerol acylhydrolase
title_sort Trichomonas vaginalis: IdentiWcation of a triacylglycerol acylhydrolase
author Carvalho, Sandra Elisa
author_facet Carvalho, Sandra Elisa
Freitas, Daniel
Murad, André Melro
Franco, Octávio Luiz
Simões-Barbosa, Augusto
author_role author
author2 Freitas, Daniel
Murad, André Melro
Franco, Octávio Luiz
Simões-Barbosa, Augusto
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Carvalho, Sandra Elisa
Freitas, Daniel
Murad, André Melro
Franco, Octávio Luiz
Simões-Barbosa, Augusto
dc.subject.por.fl_str_mv Trichomonas vaginallis
Lipase
Molecular modelling
Hemolysis
Pathogenesis
topic Trichomonas vaginallis
Lipase
Molecular modelling
Hemolysis
Pathogenesis
dc.description.abstract.por.fl_txt_mv This work describes the identiWcation of a triacylglycerol lipase named TVLip directly onto blood-LB-agar plates by hemolytic screening of a Trichomonas vaginalis cDNA expression library. Sharing signiWcant similarity in the primary sequence with other lipases, the theoretical 3D structure of the TVLip was resolved. The structure reveals the predictive conserved characteristics of other lipases from EC3.1.1.3 group, although presenting one aminoacid change in the catalytic triad Ser-His-Asp. Finally, analysis of Northern blot indicates that the expression of the TVLip gene is up-regulated by iron.
dc.description.version.pt_BR.fl_txt_mv Sim
dc.description.status.pt_BR.fl_txt_mv Publicado
description This work describes the identiWcation of a triacylglycerol lipase named TVLip directly onto blood-LB-agar plates by hemolytic screening of a Trichomonas vaginalis cDNA expression library. Sharing signiWcant similarity in the primary sequence with other lipases, the theoretical 3D structure of the TVLip was resolved. The structure reveals the predictive conserved characteristics of other lipases from EC3.1.1.3 group, although presenting one aminoacid change in the catalytic triad Ser-His-Asp. Finally, analysis of Northern blot indicates that the expression of the TVLip gene is up-regulated by iron.
publishDate 2005
dc.date.issued.fl_str_mv 2005
dc.date.accessioned.fl_str_mv 2016-10-10T03:52:49Z
dc.date.available.fl_str_mv 2016-10-10T03:52:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
status_str publishedVersion
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dc.identifier.citation.fl_str_mv CARVALHO, Sandra Elisa et al. Trichomonas vaginalis: Identification of a triacylglycerol acylhydrolase . Experimental Parasitology, v. 111, p. 260-263, 2005.
dc.identifier.uri.fl_str_mv http://twingo.ucb.br:8080/jspui/handle/10869/589
https://repositorio.ucb.br:9443/jspui/handle/123456789/7831
dc.identifier.issn.none.fl_str_mv 00144894
identifier_str_mv CARVALHO, Sandra Elisa et al. Trichomonas vaginalis: Identification of a triacylglycerol acylhydrolase . Experimental Parasitology, v. 111, p. 260-263, 2005.
00144894
url http://twingo.ucb.br:8080/jspui/handle/10869/589
https://repositorio.ucb.br:9443/jspui/handle/123456789/7831
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language eng
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