Protective effects of a cysteine proteinase propeptide expressed in transgenic soybean roots

Detalhes bibliográficos
Autor(a) principal: Marra, Brener M.
Data de Publicação: 2009
Outros Autores: Souza, Djair S.L., Aguiar, João N., Firmino, Alexandre A.P., Sarto, Rafael P.D., Silva, Francine B., Almeida, Charles D.S., Cares, Juvenil E., Continho, Marise V., Martins-de-Sa, Cezar, Franco, Octavio L., Grossi-de-Sa, Maria F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UCB
Texto Completo: http://twingo.ucb.br:8080/jspui/handle/10869/552
https://repositorio.ucb.br:9443/jspui/handle/123456789/7664
Resumo: Sedentary endoparasitic nematodes cause extensive damage to a large number of ornamental plants and food crops, with estimated economical losses over 100 billion US$ worldwide. Various efforts have put forth in order to minimize nematode damage, which typically involve the use of nematicides that have high cost and enhanced toxicity to humans and the environment. Additionally, different strategies have been applied in order to develop genetically modified plants with improved nematode resistance. Among the strategies are anti-invasion and migration, feeding-cell attenuation, and anti-nematode feeding. In the present study, we focus on anti-nematode feeding, which involves the evaluation and potential use of the cysteine proteinase (CPs) propeptide as a control alternative. The cysteine proteinase prodomain, isolated from Heterodera glycines (HGCP prodomain), is a natural inhibitory peptide used to transform soybean cotyledons using Agrobacterium rhizogenes. Genetically modified soybean roots expressing the propeptide were detected by Western blot and expression levels were measured by ELISA (around 0.3%). The transgenic roots expressing the propeptide were inoculated with a thousand H. glycines at the second juvenile stage, and a remarkable reduction in the number of females and eggs was observed. A reduction of female length and diameter was also observed after 35 days post-inoculation. Furthermore, the H. glycines mature protein was detected in females fed on soybean transformed root expressing or not expressing the propeptide. The data presented here indicate that the HGCP propeptide can reduce soybean cyst nematode infection and this strategy could be applied in the near future to generate resistant crop cultivars.
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spelling Marra, Brener M.Souza, Djair S.L.Aguiar, João N.Firmino, Alexandre A.P.Sarto, Rafael P.D.Silva, Francine B.Almeida, Charles D.S.Cares, Juvenil E.Continho, Marise V.Martins-de-Sa, CezarFranco, Octavio L.Grossi-de-Sa, Maria F.2016-10-10T03:52:17Z2016-10-10T03:52:17Z2009MARRA, Brener M. et al. Protective effects of a cysteine proteinase propeptide expressed in transgenic soybean roots. Peptidies, v. 30, n. 5, p. 825-831, 2009.http://twingo.ucb.br:8080/jspui/handle/10869/552https://repositorio.ucb.br:9443/jspui/handle/123456789/7664Sedentary endoparasitic nematodes cause extensive damage to a large number of ornamental plants and food crops, with estimated economical losses over 100 billion US$ worldwide. Various efforts have put forth in order to minimize nematode damage, which typically involve the use of nematicides that have high cost and enhanced toxicity to humans and the environment. Additionally, different strategies have been applied in order to develop genetically modified plants with improved nematode resistance. Among the strategies are anti-invasion and migration, feeding-cell attenuation, and anti-nematode feeding. In the present study, we focus on anti-nematode feeding, which involves the evaluation and potential use of the cysteine proteinase (CPs) propeptide as a control alternative. The cysteine proteinase prodomain, isolated from Heterodera glycines (HGCP prodomain), is a natural inhibitory peptide used to transform soybean cotyledons using Agrobacterium rhizogenes. Genetically modified soybean roots expressing the propeptide were detected by Western blot and expression levels were measured by ELISA (around 0.3%). The transgenic roots expressing the propeptide were inoculated with a thousand H. glycines at the second juvenile stage, and a remarkable reduction in the number of females and eggs was observed. A reduction of female length and diameter was also observed after 35 days post-inoculation. Furthermore, the H. glycines mature protein was detected in females fed on soybean transformed root expressing or not expressing the propeptide. The data presented here indicate that the HGCP propeptide can reduce soybean cyst nematode infection and this strategy could be applied in the near future to generate resistant crop cultivars.Made available in DSpace on 2016-10-10T03:52:17Z (GMT). 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dc.title.pt_BR.fl_str_mv Protective effects of a cysteine proteinase propeptide expressed in transgenic soybean roots
title Protective effects of a cysteine proteinase propeptide expressed in transgenic soybean roots
spellingShingle Protective effects of a cysteine proteinase propeptide expressed in transgenic soybean roots
Marra, Brener M.
Nematode
Cysteine proteinase
Transgenic plants
Propeptide
Heterodera glycines
title_short Protective effects of a cysteine proteinase propeptide expressed in transgenic soybean roots
title_full Protective effects of a cysteine proteinase propeptide expressed in transgenic soybean roots
title_fullStr Protective effects of a cysteine proteinase propeptide expressed in transgenic soybean roots
title_full_unstemmed Protective effects of a cysteine proteinase propeptide expressed in transgenic soybean roots
title_sort Protective effects of a cysteine proteinase propeptide expressed in transgenic soybean roots
author Marra, Brener M.
author_facet Marra, Brener M.
Souza, Djair S.L.
Aguiar, João N.
Firmino, Alexandre A.P.
Sarto, Rafael P.D.
Silva, Francine B.
Almeida, Charles D.S.
Cares, Juvenil E.
Continho, Marise V.
Martins-de-Sa, Cezar
Franco, Octavio L.
Grossi-de-Sa, Maria F.
author_role author
author2 Souza, Djair S.L.
Aguiar, João N.
Firmino, Alexandre A.P.
Sarto, Rafael P.D.
Silva, Francine B.
Almeida, Charles D.S.
Cares, Juvenil E.
Continho, Marise V.
Martins-de-Sa, Cezar
Franco, Octavio L.
Grossi-de-Sa, Maria F.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Marra, Brener M.
Souza, Djair S.L.
Aguiar, João N.
Firmino, Alexandre A.P.
Sarto, Rafael P.D.
Silva, Francine B.
Almeida, Charles D.S.
Cares, Juvenil E.
Continho, Marise V.
Martins-de-Sa, Cezar
Franco, Octavio L.
Grossi-de-Sa, Maria F.
dc.subject.por.fl_str_mv Nematode
Cysteine proteinase
Transgenic plants
Propeptide
Heterodera glycines
topic Nematode
Cysteine proteinase
Transgenic plants
Propeptide
Heterodera glycines
dc.description.abstract.por.fl_txt_mv Sedentary endoparasitic nematodes cause extensive damage to a large number of ornamental plants and food crops, with estimated economical losses over 100 billion US$ worldwide. Various efforts have put forth in order to minimize nematode damage, which typically involve the use of nematicides that have high cost and enhanced toxicity to humans and the environment. Additionally, different strategies have been applied in order to develop genetically modified plants with improved nematode resistance. Among the strategies are anti-invasion and migration, feeding-cell attenuation, and anti-nematode feeding. In the present study, we focus on anti-nematode feeding, which involves the evaluation and potential use of the cysteine proteinase (CPs) propeptide as a control alternative. The cysteine proteinase prodomain, isolated from Heterodera glycines (HGCP prodomain), is a natural inhibitory peptide used to transform soybean cotyledons using Agrobacterium rhizogenes. Genetically modified soybean roots expressing the propeptide were detected by Western blot and expression levels were measured by ELISA (around 0.3%). The transgenic roots expressing the propeptide were inoculated with a thousand H. glycines at the second juvenile stage, and a remarkable reduction in the number of females and eggs was observed. A reduction of female length and diameter was also observed after 35 days post-inoculation. Furthermore, the H. glycines mature protein was detected in females fed on soybean transformed root expressing or not expressing the propeptide. The data presented here indicate that the HGCP propeptide can reduce soybean cyst nematode infection and this strategy could be applied in the near future to generate resistant crop cultivars.
dc.description.version.pt_BR.fl_txt_mv Sim
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description Sedentary endoparasitic nematodes cause extensive damage to a large number of ornamental plants and food crops, with estimated economical losses over 100 billion US$ worldwide. Various efforts have put forth in order to minimize nematode damage, which typically involve the use of nematicides that have high cost and enhanced toxicity to humans and the environment. Additionally, different strategies have been applied in order to develop genetically modified plants with improved nematode resistance. Among the strategies are anti-invasion and migration, feeding-cell attenuation, and anti-nematode feeding. In the present study, we focus on anti-nematode feeding, which involves the evaluation and potential use of the cysteine proteinase (CPs) propeptide as a control alternative. The cysteine proteinase prodomain, isolated from Heterodera glycines (HGCP prodomain), is a natural inhibitory peptide used to transform soybean cotyledons using Agrobacterium rhizogenes. Genetically modified soybean roots expressing the propeptide were detected by Western blot and expression levels were measured by ELISA (around 0.3%). The transgenic roots expressing the propeptide were inoculated with a thousand H. glycines at the second juvenile stage, and a remarkable reduction in the number of females and eggs was observed. A reduction of female length and diameter was also observed after 35 days post-inoculation. Furthermore, the H. glycines mature protein was detected in females fed on soybean transformed root expressing or not expressing the propeptide. The data presented here indicate that the HGCP propeptide can reduce soybean cyst nematode infection and this strategy could be applied in the near future to generate resistant crop cultivars.
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2016-10-10T03:52:17Z
dc.date.available.fl_str_mv 2016-10-10T03:52:17Z
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dc.identifier.citation.fl_str_mv MARRA, Brener M. et al. Protective effects of a cysteine proteinase propeptide expressed in transgenic soybean roots. Peptidies, v. 30, n. 5, p. 825-831, 2009.
dc.identifier.uri.fl_str_mv http://twingo.ucb.br:8080/jspui/handle/10869/552
https://repositorio.ucb.br:9443/jspui/handle/123456789/7664
identifier_str_mv MARRA, Brener M. et al. Protective effects of a cysteine proteinase propeptide expressed in transgenic soybean roots. Peptidies, v. 30, n. 5, p. 825-831, 2009.
url http://twingo.ucb.br:8080/jspui/handle/10869/552
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