Transcriptome characterization of the dimorphic and pathogenic fungus Paracoccidioides brasiliensis by EST analysis

Detalhes bibliográficos
Autor(a) principal: Felipe, Maria Sueli Soares
Data de Publicação: 2003
Outros Autores: Andrade, Rosângela Vieira de, Silva, Silvana Petrofeza da, Maranhão, Andrea Queiroz, Torres, Fernando Araripe Gonçalves, Andrade, Patrícia Albuquerque de, Arraes, Fabrício Barbosa Monteiro, Arruda, Maricilia Conceição Cardoso de, Azevedo, M. O., Baptista, Alessandra Jorge, Bataus, Luiz Artur Mendes, Borges, Clayton Luiz, Campos, Elida Geralda, Cruz, Marcio Rojas da, Daher, Bruno Sahium, Dantas, Alessandra da Silva, Ferreira, Marisa Alvares da Silva Velloso, Ghil, Guilherme Vulpe, Jesuíno, Rosália Santos Amorim, Kyaw, Cynthia Maria, Leitão, L., Martins, Cláudia Renata Fernandes, Moraes, Lidia Maria Pepe de, Neves, Edvaldo Oliveira, Nicola, André Moraes, Alves, E. S., Parente, Juliana Alves, Pereira, Maristela, Fonseca, Marcio José Poças, Resende, Renato de Oliveira, Ribeiro, Bergmann Morais, Saldanha, Rosana Regina de, Santos, S. C., Silva-Pereira, Ildinete, Silva, Marcos Antônio dos Santos, Silveira, Erica Duarte, Soares, Renata de Bastos Ascenço, Souza, Diorge Paulo de, De-Souza, Marlene Teixeira, Andrade, Edmar Vaz de, Xavier, Mauro Aparecido de Sousa, Veiga, Henrique Pinheiro, Venancio, Emerson José, Carvalho, Maria José Albuquerque de, Oliveira, Adilton Guedes, Inoue, M. K., Almeida Junior, Nalvo Franco de, Walter, Maria Emília Machado Telles, Soares, Célia Maria de Almeida, Brigido, Marcelo de Macedo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UCB
Texto Completo: http://twingo.ucb.br:8080/jspui/handle/10869/603
https://repositorio.ucb.br:9443/jspui/handle/123456789/7870
Resumo: Paracoccidioides brasiliensis is a pathogenic fungus that undergoes a temperaturedependent cell morphology change from mycelium (22 ◦C) to yeast (36 ◦C). It is assumed that this morphological transition correlates with the infection of the human host. Our goal was to identify genes expressed in the mycelium (M) and yeast (Y) forms by EST sequencing in order to generate a partial map of the fungus transcriptome. Individual EST sequences were clustered by the CAP3 program and annotated using Blastx similarity analysis and InterPro Scan. Three different databases, GenBank nr, COG (clusters of orthologous groups) and GO (gene ontology) were used for annotation. A total of 3938 (Y = 1654 and M = 2274) ESTs were sequenced and clustered into 597 contigs and 1563 singlets, making up a total of 2160 genes, which possibly represent one-quarter of the complete gene repertoire in P. brasiliensis. From this total, 1040 were successfully annotated and 894 could be classified in 18 functional COG categories as follows: cellular metabolism (44%); information storage and processing (25%); cellular processes — cell division, posttranslational modifications, among others (19%); and genes of unknown functions (12%). Computer analysis enabled us to identify some genes potentially involved in the dimorphic transition and drug resistance. Furthermore, computer subtraction analysis revealed several genes possibly expressed in stage-specific forms of P. brasiliensis. Further analysis of these genes may provide new insights into the pathology and differentiation of P. brasiliensis. All EST sequences have been deposited in GenBank under Accession Nos CA580326–CA584263.
id UCB-2_4f0d5fef0bf40a9ce741f46492849dfd
oai_identifier_str oai:200.214.135.189:123456789/7870
network_acronym_str UCB-2
network_name_str Repositório Institucional da UCB
spelling Felipe, Maria Sueli SoaresAndrade, Rosângela Vieira deSilva, Silvana Petrofeza daMaranhão, Andrea QueirozTorres, Fernando Araripe GonçalvesAndrade, Patrícia Albuquerque deArraes, Fabrício Barbosa MonteiroArruda, Maricilia Conceição Cardoso deAzevedo, M. O.Baptista, Alessandra JorgeBataus, Luiz Artur MendesBorges, Clayton LuizCampos, Elida GeraldaCruz, Marcio Rojas daDaher, Bruno SahiumDantas, Alessandra da SilvaFerreira, Marisa Alvares da Silva VellosoGhil, Guilherme VulpeJesuíno, Rosália Santos AmorimKyaw, Cynthia MariaLeitão, L.Martins, Cláudia Renata FernandesMoraes, Lidia Maria Pepe deNeves, Edvaldo OliveiraNicola, André MoraesAlves, E. S.Parente, Juliana AlvesPereira, MaristelaFonseca, Marcio José PoçasResende, Renato de OliveiraRibeiro, Bergmann MoraisSaldanha, Rosana Regina deSantos, S. C.Silva-Pereira, IldineteSilva, Marcos Antônio dos SantosSilveira, Erica DuarteSoares, Renata de Bastos AscençoSouza, Diorge Paulo deDe-Souza, Marlene TeixeiraAndrade, Edmar Vaz deXavier, Mauro Aparecido de SousaVeiga, Henrique PinheiroVenancio, Emerson JoséCarvalho, Maria José Albuquerque deOliveira, Adilton GuedesInoue, M. K.Almeida Junior, Nalvo Franco deWalter, Maria Emília Machado TellesSoares, Célia Maria de AlmeidaBrigido, Marcelo de Macedo2016-10-10T03:52:57Z2016-10-10T03:52:57Z2003FELIPE, Maria Sueli Soares et al. Transcriptome characterization of the dimorphic and pathogenic fungus Paracoccidioides brasiliensis by EST analysis. Yeast, v. 20, n. 3, p. 263-271, 2003.0749503Xhttp://twingo.ucb.br:8080/jspui/handle/10869/603https://repositorio.ucb.br:9443/jspui/handle/123456789/7870Paracoccidioides brasiliensis is a pathogenic fungus that undergoes a temperaturedependent cell morphology change from mycelium (22 ◦C) to yeast (36 ◦C). It is assumed that this morphological transition correlates with the infection of the human host. Our goal was to identify genes expressed in the mycelium (M) and yeast (Y) forms by EST sequencing in order to generate a partial map of the fungus transcriptome. Individual EST sequences were clustered by the CAP3 program and annotated using Blastx similarity analysis and InterPro Scan. Three different databases, GenBank nr, COG (clusters of orthologous groups) and GO (gene ontology) were used for annotation. A total of 3938 (Y = 1654 and M = 2274) ESTs were sequenced and clustered into 597 contigs and 1563 singlets, making up a total of 2160 genes, which possibly represent one-quarter of the complete gene repertoire in P. brasiliensis. From this total, 1040 were successfully annotated and 894 could be classified in 18 functional COG categories as follows: cellular metabolism (44%); information storage and processing (25%); cellular processes — cell division, posttranslational modifications, among others (19%); and genes of unknown functions (12%). Computer analysis enabled us to identify some genes potentially involved in the dimorphic transition and drug resistance. Furthermore, computer subtraction analysis revealed several genes possibly expressed in stage-specific forms of P. brasiliensis. Further analysis of these genes may provide new insights into the pathology and differentiation of P. brasiliensis. All EST sequences have been deposited in GenBank under Accession Nos CA580326–CA584263.Made available in DSpace on 2016-10-10T03:52:57Z (GMT). No. of bitstreams: 5 Transcriptome characterization of the dimorphic and pathogenic fungus Paracoccidioides brasiliensis by EST analysis.pdf: 142465 bytes, checksum: 5ec8cb0b9e9ee100ba4725641046ae62 (MD5) license_url: 52 bytes, checksum: 2f32edb9c19a57e928372a33fd08dba5 (MD5) license_text: 24372 bytes, checksum: 94b0a37ff5ec51de8c55507bff4a7ff9 (MD5) license_rdf: 24623 bytes, checksum: 378d22d8fe50e084ee2f354be78cbe62 (MD5) license.txt: 1887 bytes, checksum: 445d1980f282ec865917de35a4c622f6 (MD5) Previous issue date: 2003SimPublicadoTextoESTsTranscriptomeFunctional genomicsHuman pathogenic fungusDimorphismParacocidioides brasiliensisTranscriptome characterization of the dimorphic and pathogenic fungus Paracoccidioides brasiliensis by EST analysisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleYeasthttp://vsites.unb.br/ib/cel/imol/papergenomaPb.pdfinfo:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UCBinstname:Universidade Católica de Brasília (UCB)instacron:UCBORIGINALTranscriptome characterization of the dimorphic and pathogenic fungus Paracoccidioides brasiliensis by EST analysis.pdfapplication/pdf142465https://200.214.135.178:9443/jspui/bitstream/123456789/7870/1/Transcriptome%20characterization%20of%20the%20dimorphic%20and%20pathogenic%20fungus%20Paracoccidioides%20brasiliensis%20by%20EST%20analysis.pdf5ec8cb0b9e9ee100ba4725641046ae62MD51CC-LICENSElicense_urlapplication/octet-stream52https://200.214.135.178:9443/jspui/bitstream/123456789/7870/2/license_url2f32edb9c19a57e928372a33fd08dba5MD52license_textapplication/octet-stream24372https://200.214.135.178:9443/jspui/bitstream/123456789/7870/3/license_text94b0a37ff5ec51de8c55507bff4a7ff9MD53license_rdfapplication/octet-stream24623https://200.214.135.178:9443/jspui/bitstream/123456789/7870/4/license_rdf378d22d8fe50e084ee2f354be78cbe62MD54LICENSElicense.txttext/plain1887https://200.214.135.178:9443/jspui/bitstream/123456789/7870/5/license.txt445d1980f282ec865917de35a4c622f6MD55TEXTTranscriptome characterization of the dimorphic and pathogenic fungus Paracoccidioides brasiliensis by EST analysis.pdf.txtTranscriptome characterization of the dimorphic and pathogenic fungus Paracoccidioides brasiliensis by EST analysis.pdf.txtExtracted texttext/plain37163https://200.214.135.178:9443/jspui/bitstream/123456789/7870/6/Transcriptome%20characterization%20of%20the%20dimorphic%20and%20pathogenic%20fungus%20Paracoccidioides%20brasiliensis%20by%20EST%20analysis.pdf.txt7ca5be2204a21aa0fba532c494ebd0deMD56123456789/78702017-01-17 15:11:33.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ório de Publicaçõeshttps://repositorio.ucb.br:9443/jspui/
dc.title.pt_BR.fl_str_mv Transcriptome characterization of the dimorphic and pathogenic fungus Paracoccidioides brasiliensis by EST analysis
title Transcriptome characterization of the dimorphic and pathogenic fungus Paracoccidioides brasiliensis by EST analysis
spellingShingle Transcriptome characterization of the dimorphic and pathogenic fungus Paracoccidioides brasiliensis by EST analysis
Felipe, Maria Sueli Soares
ESTs
Transcriptome
Functional genomics
Human pathogenic fungus
Dimorphism
Paracocidioides brasiliensis
title_short Transcriptome characterization of the dimorphic and pathogenic fungus Paracoccidioides brasiliensis by EST analysis
title_full Transcriptome characterization of the dimorphic and pathogenic fungus Paracoccidioides brasiliensis by EST analysis
title_fullStr Transcriptome characterization of the dimorphic and pathogenic fungus Paracoccidioides brasiliensis by EST analysis
title_full_unstemmed Transcriptome characterization of the dimorphic and pathogenic fungus Paracoccidioides brasiliensis by EST analysis
title_sort Transcriptome characterization of the dimorphic and pathogenic fungus Paracoccidioides brasiliensis by EST analysis
author Felipe, Maria Sueli Soares
author_facet Felipe, Maria Sueli Soares
Andrade, Rosângela Vieira de
Silva, Silvana Petrofeza da
Maranhão, Andrea Queiroz
Torres, Fernando Araripe Gonçalves
Andrade, Patrícia Albuquerque de
Arraes, Fabrício Barbosa Monteiro
Arruda, Maricilia Conceição Cardoso de
Azevedo, M. O.
Baptista, Alessandra Jorge
Bataus, Luiz Artur Mendes
Borges, Clayton Luiz
Campos, Elida Geralda
Cruz, Marcio Rojas da
Daher, Bruno Sahium
Dantas, Alessandra da Silva
Ferreira, Marisa Alvares da Silva Velloso
Ghil, Guilherme Vulpe
Jesuíno, Rosália Santos Amorim
Kyaw, Cynthia Maria
Leitão, L.
Martins, Cláudia Renata Fernandes
Moraes, Lidia Maria Pepe de
Neves, Edvaldo Oliveira
Nicola, André Moraes
Alves, E. S.
Parente, Juliana Alves
Pereira, Maristela
Fonseca, Marcio José Poças
Resende, Renato de Oliveira
Ribeiro, Bergmann Morais
Saldanha, Rosana Regina de
Santos, S. C.
Silva-Pereira, Ildinete
Silva, Marcos Antônio dos Santos
Silveira, Erica Duarte
Soares, Renata de Bastos Ascenço
Souza, Diorge Paulo de
De-Souza, Marlene Teixeira
Andrade, Edmar Vaz de
Xavier, Mauro Aparecido de Sousa
Veiga, Henrique Pinheiro
Venancio, Emerson José
Carvalho, Maria José Albuquerque de
Oliveira, Adilton Guedes
Inoue, M. K.
Almeida Junior, Nalvo Franco de
Walter, Maria Emília Machado Telles
Soares, Célia Maria de Almeida
Brigido, Marcelo de Macedo
author_role author
author2 Andrade, Rosângela Vieira de
Silva, Silvana Petrofeza da
Maranhão, Andrea Queiroz
Torres, Fernando Araripe Gonçalves
Andrade, Patrícia Albuquerque de
Arraes, Fabrício Barbosa Monteiro
Arruda, Maricilia Conceição Cardoso de
Azevedo, M. O.
Baptista, Alessandra Jorge
Bataus, Luiz Artur Mendes
Borges, Clayton Luiz
Campos, Elida Geralda
Cruz, Marcio Rojas da
Daher, Bruno Sahium
Dantas, Alessandra da Silva
Ferreira, Marisa Alvares da Silva Velloso
Ghil, Guilherme Vulpe
Jesuíno, Rosália Santos Amorim
Kyaw, Cynthia Maria
Leitão, L.
Martins, Cláudia Renata Fernandes
Moraes, Lidia Maria Pepe de
Neves, Edvaldo Oliveira
Nicola, André Moraes
Alves, E. S.
Parente, Juliana Alves
Pereira, Maristela
Fonseca, Marcio José Poças
Resende, Renato de Oliveira
Ribeiro, Bergmann Morais
Saldanha, Rosana Regina de
Santos, S. C.
Silva-Pereira, Ildinete
Silva, Marcos Antônio dos Santos
Silveira, Erica Duarte
Soares, Renata de Bastos Ascenço
Souza, Diorge Paulo de
De-Souza, Marlene Teixeira
Andrade, Edmar Vaz de
Xavier, Mauro Aparecido de Sousa
Veiga, Henrique Pinheiro
Venancio, Emerson José
Carvalho, Maria José Albuquerque de
Oliveira, Adilton Guedes
Inoue, M. K.
Almeida Junior, Nalvo Franco de
Walter, Maria Emília Machado Telles
Soares, Célia Maria de Almeida
Brigido, Marcelo de Macedo
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Felipe, Maria Sueli Soares
Andrade, Rosângela Vieira de
Silva, Silvana Petrofeza da
Maranhão, Andrea Queiroz
Torres, Fernando Araripe Gonçalves
Andrade, Patrícia Albuquerque de
Arraes, Fabrício Barbosa Monteiro
Arruda, Maricilia Conceição Cardoso de
Azevedo, M. O.
Baptista, Alessandra Jorge
Bataus, Luiz Artur Mendes
Borges, Clayton Luiz
Campos, Elida Geralda
Cruz, Marcio Rojas da
Daher, Bruno Sahium
Dantas, Alessandra da Silva
Ferreira, Marisa Alvares da Silva Velloso
Ghil, Guilherme Vulpe
Jesuíno, Rosália Santos Amorim
Kyaw, Cynthia Maria
Leitão, L.
Martins, Cláudia Renata Fernandes
Moraes, Lidia Maria Pepe de
Neves, Edvaldo Oliveira
Nicola, André Moraes
Alves, E. S.
Parente, Juliana Alves
Pereira, Maristela
Fonseca, Marcio José Poças
Resende, Renato de Oliveira
Ribeiro, Bergmann Morais
Saldanha, Rosana Regina de
Santos, S. C.
Silva-Pereira, Ildinete
Silva, Marcos Antônio dos Santos
Silveira, Erica Duarte
Soares, Renata de Bastos Ascenço
Souza, Diorge Paulo de
De-Souza, Marlene Teixeira
Andrade, Edmar Vaz de
Xavier, Mauro Aparecido de Sousa
Veiga, Henrique Pinheiro
Venancio, Emerson José
Carvalho, Maria José Albuquerque de
Oliveira, Adilton Guedes
Inoue, M. K.
Almeida Junior, Nalvo Franco de
Walter, Maria Emília Machado Telles
Soares, Célia Maria de Almeida
Brigido, Marcelo de Macedo
dc.subject.por.fl_str_mv ESTs
Transcriptome
Functional genomics
Human pathogenic fungus
Dimorphism
Paracocidioides brasiliensis
topic ESTs
Transcriptome
Functional genomics
Human pathogenic fungus
Dimorphism
Paracocidioides brasiliensis
dc.description.abstract.por.fl_txt_mv Paracoccidioides brasiliensis is a pathogenic fungus that undergoes a temperaturedependent cell morphology change from mycelium (22 ◦C) to yeast (36 ◦C). It is assumed that this morphological transition correlates with the infection of the human host. Our goal was to identify genes expressed in the mycelium (M) and yeast (Y) forms by EST sequencing in order to generate a partial map of the fungus transcriptome. Individual EST sequences were clustered by the CAP3 program and annotated using Blastx similarity analysis and InterPro Scan. Three different databases, GenBank nr, COG (clusters of orthologous groups) and GO (gene ontology) were used for annotation. A total of 3938 (Y = 1654 and M = 2274) ESTs were sequenced and clustered into 597 contigs and 1563 singlets, making up a total of 2160 genes, which possibly represent one-quarter of the complete gene repertoire in P. brasiliensis. From this total, 1040 were successfully annotated and 894 could be classified in 18 functional COG categories as follows: cellular metabolism (44%); information storage and processing (25%); cellular processes — cell division, posttranslational modifications, among others (19%); and genes of unknown functions (12%). Computer analysis enabled us to identify some genes potentially involved in the dimorphic transition and drug resistance. Furthermore, computer subtraction analysis revealed several genes possibly expressed in stage-specific forms of P. brasiliensis. Further analysis of these genes may provide new insights into the pathology and differentiation of P. brasiliensis. All EST sequences have been deposited in GenBank under Accession Nos CA580326–CA584263.
dc.description.version.pt_BR.fl_txt_mv Sim
dc.description.status.pt_BR.fl_txt_mv Publicado
description Paracoccidioides brasiliensis is a pathogenic fungus that undergoes a temperaturedependent cell morphology change from mycelium (22 ◦C) to yeast (36 ◦C). It is assumed that this morphological transition correlates with the infection of the human host. Our goal was to identify genes expressed in the mycelium (M) and yeast (Y) forms by EST sequencing in order to generate a partial map of the fungus transcriptome. Individual EST sequences were clustered by the CAP3 program and annotated using Blastx similarity analysis and InterPro Scan. Three different databases, GenBank nr, COG (clusters of orthologous groups) and GO (gene ontology) were used for annotation. A total of 3938 (Y = 1654 and M = 2274) ESTs were sequenced and clustered into 597 contigs and 1563 singlets, making up a total of 2160 genes, which possibly represent one-quarter of the complete gene repertoire in P. brasiliensis. From this total, 1040 were successfully annotated and 894 could be classified in 18 functional COG categories as follows: cellular metabolism (44%); information storage and processing (25%); cellular processes — cell division, posttranslational modifications, among others (19%); and genes of unknown functions (12%). Computer analysis enabled us to identify some genes potentially involved in the dimorphic transition and drug resistance. Furthermore, computer subtraction analysis revealed several genes possibly expressed in stage-specific forms of P. brasiliensis. Further analysis of these genes may provide new insights into the pathology and differentiation of P. brasiliensis. All EST sequences have been deposited in GenBank under Accession Nos CA580326–CA584263.
publishDate 2003
dc.date.issued.fl_str_mv 2003
dc.date.accessioned.fl_str_mv 2016-10-10T03:52:57Z
dc.date.available.fl_str_mv 2016-10-10T03:52:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
status_str publishedVersion
format article
dc.identifier.citation.fl_str_mv FELIPE, Maria Sueli Soares et al. Transcriptome characterization of the dimorphic and pathogenic fungus Paracoccidioides brasiliensis by EST analysis. Yeast, v. 20, n. 3, p. 263-271, 2003.
dc.identifier.uri.fl_str_mv http://twingo.ucb.br:8080/jspui/handle/10869/603
https://repositorio.ucb.br:9443/jspui/handle/123456789/7870
dc.identifier.issn.none.fl_str_mv 0749503X
identifier_str_mv FELIPE, Maria Sueli Soares et al. Transcriptome characterization of the dimorphic and pathogenic fungus Paracoccidioides brasiliensis by EST analysis. Yeast, v. 20, n. 3, p. 263-271, 2003.
0749503X
url http://twingo.ucb.br:8080/jspui/handle/10869/603
https://repositorio.ucb.br:9443/jspui/handle/123456789/7870
dc.language.iso.fl_str_mv eng
language eng
dc.relation.publisherversion.pt_BR.fl_str_mv http://vsites.unb.br/ib/cel/imol/papergenomaPb.pdf
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv Texto
dc.source.none.fl_str_mv reponame:Repositório Institucional da UCB
instname:Universidade Católica de Brasília (UCB)
instacron:UCB
instname_str Universidade Católica de Brasília (UCB)
instacron_str UCB
institution UCB
reponame_str Repositório Institucional da UCB
collection Repositório Institucional da UCB
bitstream.url.fl_str_mv https://200.214.135.178:9443/jspui/bitstream/123456789/7870/1/Transcriptome%20characterization%20of%20the%20dimorphic%20and%20pathogenic%20fungus%20Paracoccidioides%20brasiliensis%20by%20EST%20analysis.pdf
https://200.214.135.178:9443/jspui/bitstream/123456789/7870/2/license_url
https://200.214.135.178:9443/jspui/bitstream/123456789/7870/3/license_text
https://200.214.135.178:9443/jspui/bitstream/123456789/7870/4/license_rdf
https://200.214.135.178:9443/jspui/bitstream/123456789/7870/5/license.txt
https://200.214.135.178:9443/jspui/bitstream/123456789/7870/6/Transcriptome%20characterization%20of%20the%20dimorphic%20and%20pathogenic%20fungus%20Paracoccidioides%20brasiliensis%20by%20EST%20analysis.pdf.txt
bitstream.checksum.fl_str_mv 5ec8cb0b9e9ee100ba4725641046ae62
2f32edb9c19a57e928372a33fd08dba5
94b0a37ff5ec51de8c55507bff4a7ff9
378d22d8fe50e084ee2f354be78cbe62
445d1980f282ec865917de35a4c622f6
7ca5be2204a21aa0fba532c494ebd0de
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
MD5
MD5
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1724829832173322240