Analysis of genetic relationships of Central American and Mexican pines using RAPD markers that distinguish species
Autor(a) principal: | |
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Data de Publicação: | 1997 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UCB |
Texto Completo: | http://twingo.ucb.br:8080/jspui/handle/10869/679 https://repositorio.ucb.br:9443/jspui/handle/123456789/7830 |
Resumo: | Phylogenetic relationships were inferred for six Central American and Mexican pine species by analysing RAPD marker differences among pooled DNA samples. This population level pooling strategy discounts low-frequency allelic variation within taxa, thus obtaining a ‘cumulative genotype’ to compare among taxa. We used the morphologically based taxonomy of pines as the basis for inference concerning molecular marker divergence. Only RAPD polymorphisms that were repeatable and intensely amplified were used. The resulting data set was found to have strong hierarchical structure. Phylogenetic analyses were carried out using both neighbour-joining and maximum parsimony. The phylogenetic relationships indicated by analysis of the pine RAPD data provide new insights on pine taxonomy. The phylogenetic analyses of the RAPD marker data placed Pinus tecunumanii and P. patula clearly into two different subgroups, strongly supporting the classification of P. tecunumanii as a distinct species, and raise a new set of issues concerning the distinctions between Pinus tecunumanii and P. caribaea. Phylogenetically informative RAPD markers will be useful tools to address ex-situ conservation issues that involve taxonomic identification and species admixture. |
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Furman, Burford J.Grattapaglia, DarioDvorak, William S.O'Malley, David M.2016-10-10T03:52:49Z2016-10-10T03:52:49Z1997FURMAN, Burford J. et al.Analysis of genetic relationships of central american and mexican pines using RAPD markers that distinguish species. Molecular Ecology, v. 6, n. 4, p. 321-332, 1997.http://twingo.ucb.br:8080/jspui/handle/10869/679https://repositorio.ucb.br:9443/jspui/handle/123456789/7830Phylogenetic relationships were inferred for six Central American and Mexican pine species by analysing RAPD marker differences among pooled DNA samples. This population level pooling strategy discounts low-frequency allelic variation within taxa, thus obtaining a ‘cumulative genotype’ to compare among taxa. We used the morphologically based taxonomy of pines as the basis for inference concerning molecular marker divergence. Only RAPD polymorphisms that were repeatable and intensely amplified were used. The resulting data set was found to have strong hierarchical structure. Phylogenetic analyses were carried out using both neighbour-joining and maximum parsimony. The phylogenetic relationships indicated by analysis of the pine RAPD data provide new insights on pine taxonomy. The phylogenetic analyses of the RAPD marker data placed Pinus tecunumanii and P. patula clearly into two different subgroups, strongly supporting the classification of P. tecunumanii as a distinct species, and raise a new set of issues concerning the distinctions between Pinus tecunumanii and P. caribaea. Phylogenetically informative RAPD markers will be useful tools to address ex-situ conservation issues that involve taxonomic identification and species admixture.Made available in DSpace on 2016-10-10T03:52:49Z (GMT). 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de Publicaçõeshttps://repositorio.ucb.br:9443/jspui/ |
dc.title.pt_BR.fl_str_mv |
Analysis of genetic relationships of Central American and Mexican pines using RAPD markers that distinguish species |
title |
Analysis of genetic relationships of Central American and Mexican pines using RAPD markers that distinguish species |
spellingShingle |
Analysis of genetic relationships of Central American and Mexican pines using RAPD markers that distinguish species Furman, Burford J. RAPD DNA pooling Pinus Phylogenetics Taxonomy Tropical forestry |
title_short |
Analysis of genetic relationships of Central American and Mexican pines using RAPD markers that distinguish species |
title_full |
Analysis of genetic relationships of Central American and Mexican pines using RAPD markers that distinguish species |
title_fullStr |
Analysis of genetic relationships of Central American and Mexican pines using RAPD markers that distinguish species |
title_full_unstemmed |
Analysis of genetic relationships of Central American and Mexican pines using RAPD markers that distinguish species |
title_sort |
Analysis of genetic relationships of Central American and Mexican pines using RAPD markers that distinguish species |
author |
Furman, Burford J. |
author_facet |
Furman, Burford J. Grattapaglia, Dario Dvorak, William S. O'Malley, David M. |
author_role |
author |
author2 |
Grattapaglia, Dario Dvorak, William S. O'Malley, David M. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Furman, Burford J. Grattapaglia, Dario Dvorak, William S. O'Malley, David M. |
dc.subject.por.fl_str_mv |
RAPD DNA pooling Pinus Phylogenetics Taxonomy Tropical forestry |
topic |
RAPD DNA pooling Pinus Phylogenetics Taxonomy Tropical forestry |
dc.description.abstract.por.fl_txt_mv |
Phylogenetic relationships were inferred for six Central American and Mexican pine species by analysing RAPD marker differences among pooled DNA samples. This population level pooling strategy discounts low-frequency allelic variation within taxa, thus obtaining a ‘cumulative genotype’ to compare among taxa. We used the morphologically based taxonomy of pines as the basis for inference concerning molecular marker divergence. Only RAPD polymorphisms that were repeatable and intensely amplified were used. The resulting data set was found to have strong hierarchical structure. Phylogenetic analyses were carried out using both neighbour-joining and maximum parsimony. The phylogenetic relationships indicated by analysis of the pine RAPD data provide new insights on pine taxonomy. The phylogenetic analyses of the RAPD marker data placed Pinus tecunumanii and P. patula clearly into two different subgroups, strongly supporting the classification of P. tecunumanii as a distinct species, and raise a new set of issues concerning the distinctions between Pinus tecunumanii and P. caribaea. Phylogenetically informative RAPD markers will be useful tools to address ex-situ conservation issues that involve taxonomic identification and species admixture. |
dc.description.status.pt_BR.fl_txt_mv |
Publicado |
description |
Phylogenetic relationships were inferred for six Central American and Mexican pine species by analysing RAPD marker differences among pooled DNA samples. This population level pooling strategy discounts low-frequency allelic variation within taxa, thus obtaining a ‘cumulative genotype’ to compare among taxa. We used the morphologically based taxonomy of pines as the basis for inference concerning molecular marker divergence. Only RAPD polymorphisms that were repeatable and intensely amplified were used. The resulting data set was found to have strong hierarchical structure. Phylogenetic analyses were carried out using both neighbour-joining and maximum parsimony. The phylogenetic relationships indicated by analysis of the pine RAPD data provide new insights on pine taxonomy. The phylogenetic analyses of the RAPD marker data placed Pinus tecunumanii and P. patula clearly into two different subgroups, strongly supporting the classification of P. tecunumanii as a distinct species, and raise a new set of issues concerning the distinctions between Pinus tecunumanii and P. caribaea. Phylogenetically informative RAPD markers will be useful tools to address ex-situ conservation issues that involve taxonomic identification and species admixture. |
publishDate |
1997 |
dc.date.issued.fl_str_mv |
1997 |
dc.date.accessioned.fl_str_mv |
2016-10-10T03:52:49Z |
dc.date.available.fl_str_mv |
2016-10-10T03:52:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
status_str |
publishedVersion |
format |
article |
dc.identifier.citation.fl_str_mv |
FURMAN, Burford J. et al.Analysis of genetic relationships of central american and mexican pines using RAPD markers that distinguish species. Molecular Ecology, v. 6, n. 4, p. 321-332, 1997. |
dc.identifier.uri.fl_str_mv |
http://twingo.ucb.br:8080/jspui/handle/10869/679 https://repositorio.ucb.br:9443/jspui/handle/123456789/7830 |
identifier_str_mv |
FURMAN, Burford J. et al.Analysis of genetic relationships of central american and mexican pines using RAPD markers that distinguish species. Molecular Ecology, v. 6, n. 4, p. 321-332, 1997. |
url |
http://twingo.ucb.br:8080/jspui/handle/10869/679 https://repositorio.ucb.br:9443/jspui/handle/123456789/7830 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Católica de Brasília (UCB) |
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UCB |
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UCB |
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Repositório Institucional da UCB |
collection |
Repositório Institucional da UCB |
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