The highly expressed yeast gene pby20 from Paracoccidioides brasiliensis encodes a Xavodoxin-like protein
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UCB |
Texto Completo: | http://twingo.ucb.br:8080/jspui/handle/10869/594 https://repositorio.ucb.br:9443/jspui/handle/123456789/7854 |
Resumo: | A gene encoding the entire highly expressed protein previously identiWed in the proteome of Paracoccidioides brasiliensis yeast cells as PbY20 has been isolated. The pby20 sequence reveals an open reading frame of 1364 bp and a deduced amino acid sequence of 203 residues, which shows high identity to benzoquinone reductase from Phanerochaete chrysosporium (72.0%), Saccharomyces cerevisiae Ycp4 (65%), and Schizosaccharomyces pombe p25 (59%), and to allergens from Alternaria alternata Alt a7 (70%) and from Cladosporium herbarum, Cla h5 (68%). Low levels of the pby20 transcript in the mycelium and highly induced ones in infective yeast cells during the transition of this dimorphic fungus indicate transcriptional control of its expression. PbY20 was immunologically detected only in yeast cell extract, suggesting an important role in cell diVerentiation or even in the maintenance of the yeast form. Immunoelectron microscopy showed that PbY20 is found inside large granules and vacuoles, in the nucleus, and also in the cytoplasm. Through sequence comparisons analysis and Xuorescence emission assay, PbY20 was recognized as a member of the Xavin mononucleotide Xavodoxin-like WrbA family, which are involved in heat shock and oxidative stress in biological systems. Assuming that PbY20 belongs to this family, a similar role could be attributed to this protein. |
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Daher, Bruno SahiumVenancio, Emerson JoséFreitas, Sonia Maria deBáo, Sônia NairVilela, Paulo Vianney RodriguesAndrade, Rosângela Vieira deDantas, Alessandra da SilvaSoares, Célia Maria de AlmeidaSilva-Pereira, IldineteFelipe, Maria Sueli Soares2016-10-10T03:52:54Z2016-10-10T03:52:54Z2005DAHER, Bruno Sahium et al. The highly expressed yeast gene pby20 from Paracoccidioides brasiliensis encodes a Xavodoxin-like protein. Fungal Genetics and Biology, v. 42, p. 434–443, 2005.http://twingo.ucb.br:8080/jspui/handle/10869/594https://repositorio.ucb.br:9443/jspui/handle/123456789/7854A gene encoding the entire highly expressed protein previously identiWed in the proteome of Paracoccidioides brasiliensis yeast cells as PbY20 has been isolated. The pby20 sequence reveals an open reading frame of 1364 bp and a deduced amino acid sequence of 203 residues, which shows high identity to benzoquinone reductase from Phanerochaete chrysosporium (72.0%), Saccharomyces cerevisiae Ycp4 (65%), and Schizosaccharomyces pombe p25 (59%), and to allergens from Alternaria alternata Alt a7 (70%) and from Cladosporium herbarum, Cla h5 (68%). Low levels of the pby20 transcript in the mycelium and highly induced ones in infective yeast cells during the transition of this dimorphic fungus indicate transcriptional control of its expression. PbY20 was immunologically detected only in yeast cell extract, suggesting an important role in cell diVerentiation or even in the maintenance of the yeast form. Immunoelectron microscopy showed that PbY20 is found inside large granules and vacuoles, in the nucleus, and also in the cytoplasm. Through sequence comparisons analysis and Xuorescence emission assay, PbY20 was recognized as a member of the Xavin mononucleotide Xavodoxin-like WrbA family, which are involved in heat shock and oxidative stress in biological systems. Assuming that PbY20 belongs to this family, a similar role could be attributed to this protein.Made available in DSpace on 2016-10-10T03:52:54Z (GMT). No. of bitstreams: 5 The highly expressed yeast gene pby20 from Paracoccidioides brasiliensis encodes a flavodoxin_like protein.pdf: 1132850 bytes, checksum: bcdb1c3d656cec34fbab7c6961b7743a (MD5) license_url: 52 bytes, checksum: 2f32edb9c19a57e928372a33fd08dba5 (MD5) license_text: 24372 bytes, checksum: 94b0a37ff5ec51de8c55507bff4a7ff9 (MD5) license_rdf: 24623 bytes, checksum: 378d22d8fe50e084ee2f354be78cbe62 (MD5) license.txt: 1887 bytes, checksum: 445d1980f282ec865917de35a4c622f6 (MD5) Previous issue date: 2005SimPublicadoTextoRestrito UCBinfo:eu-repo/semantics/openAccessDimorphic fungusOverexpressed yeast proteinWrbA familyFMN-bindingFlavodoxinQuinone reductaseThe highly expressed yeast gene pby20 from Paracoccidioides brasiliensis encodes a Xavodoxin-like proteininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFungal Genetics and 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de Publicaçõeshttps://repositorio.ucb.br:9443/jspui/ |
dc.title.pt_BR.fl_str_mv |
The highly expressed yeast gene pby20 from Paracoccidioides brasiliensis encodes a Xavodoxin-like protein |
title |
The highly expressed yeast gene pby20 from Paracoccidioides brasiliensis encodes a Xavodoxin-like protein |
spellingShingle |
The highly expressed yeast gene pby20 from Paracoccidioides brasiliensis encodes a Xavodoxin-like protein Daher, Bruno Sahium Dimorphic fungus Overexpressed yeast protein WrbA family FMN-binding Flavodoxin Quinone reductase |
title_short |
The highly expressed yeast gene pby20 from Paracoccidioides brasiliensis encodes a Xavodoxin-like protein |
title_full |
The highly expressed yeast gene pby20 from Paracoccidioides brasiliensis encodes a Xavodoxin-like protein |
title_fullStr |
The highly expressed yeast gene pby20 from Paracoccidioides brasiliensis encodes a Xavodoxin-like protein |
title_full_unstemmed |
The highly expressed yeast gene pby20 from Paracoccidioides brasiliensis encodes a Xavodoxin-like protein |
title_sort |
The highly expressed yeast gene pby20 from Paracoccidioides brasiliensis encodes a Xavodoxin-like protein |
author |
Daher, Bruno Sahium |
author_facet |
Daher, Bruno Sahium Venancio, Emerson José Freitas, Sonia Maria de Báo, Sônia Nair Vilela, Paulo Vianney Rodrigues Andrade, Rosângela Vieira de Dantas, Alessandra da Silva Soares, Célia Maria de Almeida Silva-Pereira, Ildinete Felipe, Maria Sueli Soares |
author_role |
author |
author2 |
Venancio, Emerson José Freitas, Sonia Maria de Báo, Sônia Nair Vilela, Paulo Vianney Rodrigues Andrade, Rosângela Vieira de Dantas, Alessandra da Silva Soares, Célia Maria de Almeida Silva-Pereira, Ildinete Felipe, Maria Sueli Soares |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Daher, Bruno Sahium Venancio, Emerson José Freitas, Sonia Maria de Báo, Sônia Nair Vilela, Paulo Vianney Rodrigues Andrade, Rosângela Vieira de Dantas, Alessandra da Silva Soares, Célia Maria de Almeida Silva-Pereira, Ildinete Felipe, Maria Sueli Soares |
dc.subject.por.fl_str_mv |
Dimorphic fungus Overexpressed yeast protein WrbA family FMN-binding Flavodoxin Quinone reductase |
topic |
Dimorphic fungus Overexpressed yeast protein WrbA family FMN-binding Flavodoxin Quinone reductase |
dc.description.abstract.por.fl_txt_mv |
A gene encoding the entire highly expressed protein previously identiWed in the proteome of Paracoccidioides brasiliensis yeast cells as PbY20 has been isolated. The pby20 sequence reveals an open reading frame of 1364 bp and a deduced amino acid sequence of 203 residues, which shows high identity to benzoquinone reductase from Phanerochaete chrysosporium (72.0%), Saccharomyces cerevisiae Ycp4 (65%), and Schizosaccharomyces pombe p25 (59%), and to allergens from Alternaria alternata Alt a7 (70%) and from Cladosporium herbarum, Cla h5 (68%). Low levels of the pby20 transcript in the mycelium and highly induced ones in infective yeast cells during the transition of this dimorphic fungus indicate transcriptional control of its expression. PbY20 was immunologically detected only in yeast cell extract, suggesting an important role in cell diVerentiation or even in the maintenance of the yeast form. Immunoelectron microscopy showed that PbY20 is found inside large granules and vacuoles, in the nucleus, and also in the cytoplasm. Through sequence comparisons analysis and Xuorescence emission assay, PbY20 was recognized as a member of the Xavin mononucleotide Xavodoxin-like WrbA family, which are involved in heat shock and oxidative stress in biological systems. Assuming that PbY20 belongs to this family, a similar role could be attributed to this protein. |
dc.description.version.pt_BR.fl_txt_mv |
Sim |
dc.description.status.pt_BR.fl_txt_mv |
Publicado |
description |
A gene encoding the entire highly expressed protein previously identiWed in the proteome of Paracoccidioides brasiliensis yeast cells as PbY20 has been isolated. The pby20 sequence reveals an open reading frame of 1364 bp and a deduced amino acid sequence of 203 residues, which shows high identity to benzoquinone reductase from Phanerochaete chrysosporium (72.0%), Saccharomyces cerevisiae Ycp4 (65%), and Schizosaccharomyces pombe p25 (59%), and to allergens from Alternaria alternata Alt a7 (70%) and from Cladosporium herbarum, Cla h5 (68%). Low levels of the pby20 transcript in the mycelium and highly induced ones in infective yeast cells during the transition of this dimorphic fungus indicate transcriptional control of its expression. PbY20 was immunologically detected only in yeast cell extract, suggesting an important role in cell diVerentiation or even in the maintenance of the yeast form. Immunoelectron microscopy showed that PbY20 is found inside large granules and vacuoles, in the nucleus, and also in the cytoplasm. Through sequence comparisons analysis and Xuorescence emission assay, PbY20 was recognized as a member of the Xavin mononucleotide Xavodoxin-like WrbA family, which are involved in heat shock and oxidative stress in biological systems. Assuming that PbY20 belongs to this family, a similar role could be attributed to this protein. |
publishDate |
2005 |
dc.date.issued.fl_str_mv |
2005 |
dc.date.accessioned.fl_str_mv |
2016-10-10T03:52:54Z |
dc.date.available.fl_str_mv |
2016-10-10T03:52:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
status_str |
publishedVersion |
format |
article |
dc.identifier.citation.fl_str_mv |
DAHER, Bruno Sahium et al. The highly expressed yeast gene pby20 from Paracoccidioides brasiliensis encodes a Xavodoxin-like protein. Fungal Genetics and Biology, v. 42, p. 434–443, 2005. |
dc.identifier.uri.fl_str_mv |
http://twingo.ucb.br:8080/jspui/handle/10869/594 https://repositorio.ucb.br:9443/jspui/handle/123456789/7854 |
identifier_str_mv |
DAHER, Bruno Sahium et al. The highly expressed yeast gene pby20 from Paracoccidioides brasiliensis encodes a Xavodoxin-like protein. Fungal Genetics and Biology, v. 42, p. 434–443, 2005. |
url |
http://twingo.ucb.br:8080/jspui/handle/10869/594 https://repositorio.ucb.br:9443/jspui/handle/123456789/7854 |
dc.language.iso.fl_str_mv |
eng |
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eng |
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http://natalneuro.academia.edu/paulovianneyvrodriguesrodrigues/Papers/994555/The_highly_expressed_yeast_gene_pby20_from_Paracoccidioides_brasiliensis_encodes_a_Xavodoxin-like_protein |
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openAccess |
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Repositório Institucional da UCB |
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