The highly expressed yeast gene pby20 from Paracoccidioides brasiliensis encodes a Xavodoxin-like protein

Detalhes bibliográficos
Autor(a) principal: Daher, Bruno Sahium
Data de Publicação: 2005
Outros Autores: Venancio, Emerson José, Freitas, Sonia Maria de, Báo, Sônia Nair, Vilela, Paulo Vianney Rodrigues, Andrade, Rosângela Vieira de, Dantas, Alessandra da Silva, Soares, Célia Maria de Almeida, Silva-Pereira, Ildinete, Felipe, Maria Sueli Soares
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UCB
Texto Completo: http://twingo.ucb.br:8080/jspui/handle/10869/594
https://repositorio.ucb.br:9443/jspui/handle/123456789/7854
Resumo: A gene encoding the entire highly expressed protein previously identiWed in the proteome of Paracoccidioides brasiliensis yeast cells as PbY20 has been isolated. The pby20 sequence reveals an open reading frame of 1364 bp and a deduced amino acid sequence of 203 residues, which shows high identity to benzoquinone reductase from Phanerochaete chrysosporium (72.0%), Saccharomyces cerevisiae Ycp4 (65%), and Schizosaccharomyces pombe p25 (59%), and to allergens from Alternaria alternata Alt a7 (70%) and from Cladosporium herbarum, Cla h5 (68%). Low levels of the pby20 transcript in the mycelium and highly induced ones in infective yeast cells during the transition of this dimorphic fungus indicate transcriptional control of its expression. PbY20 was immunologically detected only in yeast cell extract, suggesting an important role in cell diVerentiation or even in the maintenance of the yeast form. Immunoelectron microscopy showed that PbY20 is found inside large granules and vacuoles, in the nucleus, and also in the cytoplasm. Through sequence comparisons analysis and Xuorescence emission assay, PbY20 was recognized as a member of the Xavin mononucleotide Xavodoxin-like WrbA family, which are involved in heat shock and oxidative stress in biological systems. Assuming that PbY20 belongs to this family, a similar role could be attributed to this protein.
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spelling Daher, Bruno SahiumVenancio, Emerson JoséFreitas, Sonia Maria deBáo, Sônia NairVilela, Paulo Vianney RodriguesAndrade, Rosângela Vieira deDantas, Alessandra da SilvaSoares, Célia Maria de AlmeidaSilva-Pereira, IldineteFelipe, Maria Sueli Soares2016-10-10T03:52:54Z2016-10-10T03:52:54Z2005DAHER, Bruno Sahium et al. The highly expressed yeast gene pby20 from Paracoccidioides brasiliensis encodes a Xavodoxin-like protein. Fungal Genetics and Biology, v. 42, p. 434–443, 2005.http://twingo.ucb.br:8080/jspui/handle/10869/594https://repositorio.ucb.br:9443/jspui/handle/123456789/7854A gene encoding the entire highly expressed protein previously identiWed in the proteome of Paracoccidioides brasiliensis yeast cells as PbY20 has been isolated. The pby20 sequence reveals an open reading frame of 1364 bp and a deduced amino acid sequence of 203 residues, which shows high identity to benzoquinone reductase from Phanerochaete chrysosporium (72.0%), Saccharomyces cerevisiae Ycp4 (65%), and Schizosaccharomyces pombe p25 (59%), and to allergens from Alternaria alternata Alt a7 (70%) and from Cladosporium herbarum, Cla h5 (68%). Low levels of the pby20 transcript in the mycelium and highly induced ones in infective yeast cells during the transition of this dimorphic fungus indicate transcriptional control of its expression. PbY20 was immunologically detected only in yeast cell extract, suggesting an important role in cell diVerentiation or even in the maintenance of the yeast form. Immunoelectron microscopy showed that PbY20 is found inside large granules and vacuoles, in the nucleus, and also in the cytoplasm. Through sequence comparisons analysis and Xuorescence emission assay, PbY20 was recognized as a member of the Xavin mononucleotide Xavodoxin-like WrbA family, which are involved in heat shock and oxidative stress in biological systems. Assuming that PbY20 belongs to this family, a similar role could be attributed to this protein.Made available in DSpace on 2016-10-10T03:52:54Z (GMT). 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dc.title.pt_BR.fl_str_mv The highly expressed yeast gene pby20 from Paracoccidioides brasiliensis encodes a Xavodoxin-like protein
title The highly expressed yeast gene pby20 from Paracoccidioides brasiliensis encodes a Xavodoxin-like protein
spellingShingle The highly expressed yeast gene pby20 from Paracoccidioides brasiliensis encodes a Xavodoxin-like protein
Daher, Bruno Sahium
Dimorphic fungus
Overexpressed yeast protein
WrbA family
FMN-binding
Flavodoxin
Quinone reductase
title_short The highly expressed yeast gene pby20 from Paracoccidioides brasiliensis encodes a Xavodoxin-like protein
title_full The highly expressed yeast gene pby20 from Paracoccidioides brasiliensis encodes a Xavodoxin-like protein
title_fullStr The highly expressed yeast gene pby20 from Paracoccidioides brasiliensis encodes a Xavodoxin-like protein
title_full_unstemmed The highly expressed yeast gene pby20 from Paracoccidioides brasiliensis encodes a Xavodoxin-like protein
title_sort The highly expressed yeast gene pby20 from Paracoccidioides brasiliensis encodes a Xavodoxin-like protein
author Daher, Bruno Sahium
author_facet Daher, Bruno Sahium
Venancio, Emerson José
Freitas, Sonia Maria de
Báo, Sônia Nair
Vilela, Paulo Vianney Rodrigues
Andrade, Rosângela Vieira de
Dantas, Alessandra da Silva
Soares, Célia Maria de Almeida
Silva-Pereira, Ildinete
Felipe, Maria Sueli Soares
author_role author
author2 Venancio, Emerson José
Freitas, Sonia Maria de
Báo, Sônia Nair
Vilela, Paulo Vianney Rodrigues
Andrade, Rosângela Vieira de
Dantas, Alessandra da Silva
Soares, Célia Maria de Almeida
Silva-Pereira, Ildinete
Felipe, Maria Sueli Soares
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Daher, Bruno Sahium
Venancio, Emerson José
Freitas, Sonia Maria de
Báo, Sônia Nair
Vilela, Paulo Vianney Rodrigues
Andrade, Rosângela Vieira de
Dantas, Alessandra da Silva
Soares, Célia Maria de Almeida
Silva-Pereira, Ildinete
Felipe, Maria Sueli Soares
dc.subject.por.fl_str_mv Dimorphic fungus
Overexpressed yeast protein
WrbA family
FMN-binding
Flavodoxin
Quinone reductase
topic Dimorphic fungus
Overexpressed yeast protein
WrbA family
FMN-binding
Flavodoxin
Quinone reductase
dc.description.abstract.por.fl_txt_mv A gene encoding the entire highly expressed protein previously identiWed in the proteome of Paracoccidioides brasiliensis yeast cells as PbY20 has been isolated. The pby20 sequence reveals an open reading frame of 1364 bp and a deduced amino acid sequence of 203 residues, which shows high identity to benzoquinone reductase from Phanerochaete chrysosporium (72.0%), Saccharomyces cerevisiae Ycp4 (65%), and Schizosaccharomyces pombe p25 (59%), and to allergens from Alternaria alternata Alt a7 (70%) and from Cladosporium herbarum, Cla h5 (68%). Low levels of the pby20 transcript in the mycelium and highly induced ones in infective yeast cells during the transition of this dimorphic fungus indicate transcriptional control of its expression. PbY20 was immunologically detected only in yeast cell extract, suggesting an important role in cell diVerentiation or even in the maintenance of the yeast form. Immunoelectron microscopy showed that PbY20 is found inside large granules and vacuoles, in the nucleus, and also in the cytoplasm. Through sequence comparisons analysis and Xuorescence emission assay, PbY20 was recognized as a member of the Xavin mononucleotide Xavodoxin-like WrbA family, which are involved in heat shock and oxidative stress in biological systems. Assuming that PbY20 belongs to this family, a similar role could be attributed to this protein.
dc.description.version.pt_BR.fl_txt_mv Sim
dc.description.status.pt_BR.fl_txt_mv Publicado
description A gene encoding the entire highly expressed protein previously identiWed in the proteome of Paracoccidioides brasiliensis yeast cells as PbY20 has been isolated. The pby20 sequence reveals an open reading frame of 1364 bp and a deduced amino acid sequence of 203 residues, which shows high identity to benzoquinone reductase from Phanerochaete chrysosporium (72.0%), Saccharomyces cerevisiae Ycp4 (65%), and Schizosaccharomyces pombe p25 (59%), and to allergens from Alternaria alternata Alt a7 (70%) and from Cladosporium herbarum, Cla h5 (68%). Low levels of the pby20 transcript in the mycelium and highly induced ones in infective yeast cells during the transition of this dimorphic fungus indicate transcriptional control of its expression. PbY20 was immunologically detected only in yeast cell extract, suggesting an important role in cell diVerentiation or even in the maintenance of the yeast form. Immunoelectron microscopy showed that PbY20 is found inside large granules and vacuoles, in the nucleus, and also in the cytoplasm. Through sequence comparisons analysis and Xuorescence emission assay, PbY20 was recognized as a member of the Xavin mononucleotide Xavodoxin-like WrbA family, which are involved in heat shock and oxidative stress in biological systems. Assuming that PbY20 belongs to this family, a similar role could be attributed to this protein.
publishDate 2005
dc.date.issued.fl_str_mv 2005
dc.date.accessioned.fl_str_mv 2016-10-10T03:52:54Z
dc.date.available.fl_str_mv 2016-10-10T03:52:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.citation.fl_str_mv DAHER, Bruno Sahium et al. The highly expressed yeast gene pby20 from Paracoccidioides brasiliensis encodes a Xavodoxin-like protein. Fungal Genetics and Biology, v. 42, p. 434–443, 2005.
dc.identifier.uri.fl_str_mv http://twingo.ucb.br:8080/jspui/handle/10869/594
https://repositorio.ucb.br:9443/jspui/handle/123456789/7854
identifier_str_mv DAHER, Bruno Sahium et al. The highly expressed yeast gene pby20 from Paracoccidioides brasiliensis encodes a Xavodoxin-like protein. Fungal Genetics and Biology, v. 42, p. 434–443, 2005.
url http://twingo.ucb.br:8080/jspui/handle/10869/594
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