Susceptibility of human pathogenic bacteria to antimicrobial peptides from sesame kernels
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UCB |
Texto Completo: | http://twingo.ucb.br:8080/jspui/handle/10869/398 https://repositorio.ucb.br:9443/jspui/handle/123456789/7556 |
Resumo: | Hospital infection caused by Gram-negative bacteria is a serious and common problem, especially in developing countries. Aiming to reduce these infections, this report focuses on the identification and characterization of novel antimicrobial peptides from sesame (Sesamum indicum) kernel meals. Thus, sesame flour was extracted and precipitated with ammonium sulfate (100%). After dialysis, a rich fraction was applied to affinity red-Sepharose CL-6B chromatography, followed by reversedphase high-performance liquid chromatography. Mass spectrometry analysis indicated the presence of a major peptide with molecular mass of _5.8 kDa in both cultivars. The bactericidal activities of antimicrobial peptides were evaluated against several human pathogens that had been effective only against Klebsiella sp., a Gram-negative bacterium responsible for human urinary infection. These data indicate the biotechnological potential of sesame peptides as an alternative method for hospital infection control and also the decrease of bacterial resistance to synthetic antibiotics. |
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Costa, Fábio TelesMaria Neto, SimoneBloch Jr., CarlosFranco, Octávio Luiz2016-10-10T03:51:52Z2016-10-10T03:51:52Z2007COSTA, Fabio Teles. et al. Susceptibility of human pathogenic bacteria to antimicrobial peptides. Current Microbiology, v. 55, p. 162-166, 2007.3438651http://twingo.ucb.br:8080/jspui/handle/10869/398https://repositorio.ucb.br:9443/jspui/handle/123456789/7556Hospital infection caused by Gram-negative bacteria is a serious and common problem, especially in developing countries. Aiming to reduce these infections, this report focuses on the identification and characterization of novel antimicrobial peptides from sesame (Sesamum indicum) kernel meals. Thus, sesame flour was extracted and precipitated with ammonium sulfate (100%). After dialysis, a rich fraction was applied to affinity red-Sepharose CL-6B chromatography, followed by reversedphase high-performance liquid chromatography. Mass spectrometry analysis indicated the presence of a major peptide with molecular mass of _5.8 kDa in both cultivars. The bactericidal activities of antimicrobial peptides were evaluated against several human pathogens that had been effective only against Klebsiella sp., a Gram-negative bacterium responsible for human urinary infection. These data indicate the biotechnological potential of sesame peptides as an alternative method for hospital infection control and also the decrease of bacterial resistance to synthetic antibiotics.Made available in DSpace on 2016-10-10T03:51:52Z (GMT). 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dc.title.pt_BR.fl_str_mv |
Susceptibility of human pathogenic bacteria to antimicrobial peptides from sesame kernels |
title |
Susceptibility of human pathogenic bacteria to antimicrobial peptides from sesame kernels |
spellingShingle |
Susceptibility of human pathogenic bacteria to antimicrobial peptides from sesame kernels Costa, Fábio Teles Sesamum indicum Antibiotical peptides Klebsiella |
title_short |
Susceptibility of human pathogenic bacteria to antimicrobial peptides from sesame kernels |
title_full |
Susceptibility of human pathogenic bacteria to antimicrobial peptides from sesame kernels |
title_fullStr |
Susceptibility of human pathogenic bacteria to antimicrobial peptides from sesame kernels |
title_full_unstemmed |
Susceptibility of human pathogenic bacteria to antimicrobial peptides from sesame kernels |
title_sort |
Susceptibility of human pathogenic bacteria to antimicrobial peptides from sesame kernels |
author |
Costa, Fábio Teles |
author_facet |
Costa, Fábio Teles Maria Neto, Simone Bloch Jr., Carlos Franco, Octávio Luiz |
author_role |
author |
author2 |
Maria Neto, Simone Bloch Jr., Carlos Franco, Octávio Luiz |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Costa, Fábio Teles Maria Neto, Simone Bloch Jr., Carlos Franco, Octávio Luiz |
dc.subject.por.fl_str_mv |
Sesamum indicum Antibiotical peptides Klebsiella |
topic |
Sesamum indicum Antibiotical peptides Klebsiella |
dc.description.abstract.por.fl_txt_mv |
Hospital infection caused by Gram-negative bacteria is a serious and common problem, especially in developing countries. Aiming to reduce these infections, this report focuses on the identification and characterization of novel antimicrobial peptides from sesame (Sesamum indicum) kernel meals. Thus, sesame flour was extracted and precipitated with ammonium sulfate (100%). After dialysis, a rich fraction was applied to affinity red-Sepharose CL-6B chromatography, followed by reversedphase high-performance liquid chromatography. Mass spectrometry analysis indicated the presence of a major peptide with molecular mass of _5.8 kDa in both cultivars. The bactericidal activities of antimicrobial peptides were evaluated against several human pathogens that had been effective only against Klebsiella sp., a Gram-negative bacterium responsible for human urinary infection. These data indicate the biotechnological potential of sesame peptides as an alternative method for hospital infection control and also the decrease of bacterial resistance to synthetic antibiotics. |
dc.description.version.pt_BR.fl_txt_mv |
Sim |
dc.description.status.pt_BR.fl_txt_mv |
Publicado |
description |
Hospital infection caused by Gram-negative bacteria is a serious and common problem, especially in developing countries. Aiming to reduce these infections, this report focuses on the identification and characterization of novel antimicrobial peptides from sesame (Sesamum indicum) kernel meals. Thus, sesame flour was extracted and precipitated with ammonium sulfate (100%). After dialysis, a rich fraction was applied to affinity red-Sepharose CL-6B chromatography, followed by reversedphase high-performance liquid chromatography. Mass spectrometry analysis indicated the presence of a major peptide with molecular mass of _5.8 kDa in both cultivars. The bactericidal activities of antimicrobial peptides were evaluated against several human pathogens that had been effective only against Klebsiella sp., a Gram-negative bacterium responsible for human urinary infection. These data indicate the biotechnological potential of sesame peptides as an alternative method for hospital infection control and also the decrease of bacterial resistance to synthetic antibiotics. |
publishDate |
2007 |
dc.date.issued.fl_str_mv |
2007 |
dc.date.accessioned.fl_str_mv |
2016-10-10T03:51:52Z |
dc.date.available.fl_str_mv |
2016-10-10T03:51:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
status_str |
publishedVersion |
format |
article |
dc.identifier.citation.fl_str_mv |
COSTA, Fabio Teles. et al. Susceptibility of human pathogenic bacteria to antimicrobial peptides. Current Microbiology, v. 55, p. 162-166, 2007. |
dc.identifier.uri.fl_str_mv |
http://twingo.ucb.br:8080/jspui/handle/10869/398 https://repositorio.ucb.br:9443/jspui/handle/123456789/7556 |
dc.identifier.issn.none.fl_str_mv |
3438651 |
identifier_str_mv |
COSTA, Fabio Teles. et al. Susceptibility of human pathogenic bacteria to antimicrobial peptides. Current Microbiology, v. 55, p. 162-166, 2007. 3438651 |
url |
http://twingo.ucb.br:8080/jspui/handle/10869/398 https://repositorio.ucb.br:9443/jspui/handle/123456789/7556 |
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eng |
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eng |
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openAccess |
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Universidade Católica de Brasília (UCB) |
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UCB |
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UCB |
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Repositório Institucional da UCB |
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Repositório Institucional da UCB |
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