Resistance to rust (Puccinia psidii winter) in eucalyptus: mode of inheritance and mapping of a major gene with RAPD markers
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UCB |
Texto Completo: | http://twingo.ucb.br:8080/jspui/handle/10869/521 https://repositorio.ucb.br:9443/jspui/handle/123456789/7755 |
Resumo: | Rust is one of the most-damaging eucalypt diseases in Brazil and is considered a potential threat to eucalypt plantations worldwide. To determine the mode of inheritance of esistance in the Eucalyptus grandis—Puccinia psidii pathosystem, ten full-sib families, generated from crosses between susceptible and resistant trees, were inoculated with a single-pustule isolate of the pathogen and rust severity was scored. The observed segregation ratios in segregating families suggested major gene control of rust resistance, although clearly incomplete penetrance, variable expressivity and minor genes are also involved in the global rust-resistance response. To identify markers linked to the resistance locus, screening of RAPD polymorphisms was conducted using bulked segregant analysis in a large full-sib family. A linkage group was built around the Ppr1 gene (P. psidii resistance gene 1) encompassing six RAPD markers, with a genetic window spanning 5 cM with the two mostclosely linked flanking markers. Besides these two flanking markers, RAPD marker AT9/917 co-segregated with Ppr1 without a single recombinant in 994 meioses. This tightly linked marker should prove useful for marker-assisted introgression and will provide an initial lead for a positional cloning effort of this resistance allele. This is the first report of a disease resistance gene identified in Eucalyptus, and one of the few examples of the involvement of a major gene in a non-coevolved pathosystem. |
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Junghans, Davi TheodoroAlfenas, Acelino CoutoBrommonschenkel, Sérgio HerminioOda, ShinitiroMello, Eduardo José deGrattapaglia, Dário2016-10-10T03:52:34Z2016-10-10T03:52:34Z2003JUNGHANS, Davi Theodoro et al. Resistance to rust (Puccinia psidii winter) in eucalyptus: mode of inheritance and mapping of a major gene with RAPD markers. Theor Appl Genet, v.108, p.175-180, 2003.http://twingo.ucb.br:8080/jspui/handle/10869/521https://repositorio.ucb.br:9443/jspui/handle/123456789/7755Rust is one of the most-damaging eucalypt diseases in Brazil and is considered a potential threat to eucalypt plantations worldwide. To determine the mode of inheritance of esistance in the Eucalyptus grandis—Puccinia psidii pathosystem, ten full-sib families, generated from crosses between susceptible and resistant trees, were inoculated with a single-pustule isolate of the pathogen and rust severity was scored. The observed segregation ratios in segregating families suggested major gene control of rust resistance, although clearly incomplete penetrance, variable expressivity and minor genes are also involved in the global rust-resistance response. To identify markers linked to the resistance locus, screening of RAPD polymorphisms was conducted using bulked segregant analysis in a large full-sib family. A linkage group was built around the Ppr1 gene (P. psidii resistance gene 1) encompassing six RAPD markers, with a genetic window spanning 5 cM with the two mostclosely linked flanking markers. Besides these two flanking markers, RAPD marker AT9/917 co-segregated with Ppr1 without a single recombinant in 994 meioses. This tightly linked marker should prove useful for marker-assisted introgression and will provide an initial lead for a positional cloning effort of this resistance allele. This is the first report of a disease resistance gene identified in Eucalyptus, and one of the few examples of the involvement of a major gene in a non-coevolved pathosystem.Made available in DSpace on 2016-10-10T03:52:34Z (GMT). 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dc.title.pt_BR.fl_str_mv |
Resistance to rust (Puccinia psidii winter) in eucalyptus: mode of inheritance and mapping of a major gene with RAPD markers |
title |
Resistance to rust (Puccinia psidii winter) in eucalyptus: mode of inheritance and mapping of a major gene with RAPD markers |
spellingShingle |
Resistance to rust (Puccinia psidii winter) in eucalyptus: mode of inheritance and mapping of a major gene with RAPD markers Junghans, Davi Theodoro |
title_short |
Resistance to rust (Puccinia psidii winter) in eucalyptus: mode of inheritance and mapping of a major gene with RAPD markers |
title_full |
Resistance to rust (Puccinia psidii winter) in eucalyptus: mode of inheritance and mapping of a major gene with RAPD markers |
title_fullStr |
Resistance to rust (Puccinia psidii winter) in eucalyptus: mode of inheritance and mapping of a major gene with RAPD markers |
title_full_unstemmed |
Resistance to rust (Puccinia psidii winter) in eucalyptus: mode of inheritance and mapping of a major gene with RAPD markers |
title_sort |
Resistance to rust (Puccinia psidii winter) in eucalyptus: mode of inheritance and mapping of a major gene with RAPD markers |
author |
Junghans, Davi Theodoro |
author_facet |
Junghans, Davi Theodoro Alfenas, Acelino Couto Brommonschenkel, Sérgio Herminio Oda, Shinitiro Mello, Eduardo José de Grattapaglia, Dário |
author_role |
author |
author2 |
Alfenas, Acelino Couto Brommonschenkel, Sérgio Herminio Oda, Shinitiro Mello, Eduardo José de Grattapaglia, Dário |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Junghans, Davi Theodoro Alfenas, Acelino Couto Brommonschenkel, Sérgio Herminio Oda, Shinitiro Mello, Eduardo José de Grattapaglia, Dário |
dc.description.abstract.por.fl_txt_mv |
Rust is one of the most-damaging eucalypt diseases in Brazil and is considered a potential threat to eucalypt plantations worldwide. To determine the mode of inheritance of esistance in the Eucalyptus grandis—Puccinia psidii pathosystem, ten full-sib families, generated from crosses between susceptible and resistant trees, were inoculated with a single-pustule isolate of the pathogen and rust severity was scored. The observed segregation ratios in segregating families suggested major gene control of rust resistance, although clearly incomplete penetrance, variable expressivity and minor genes are also involved in the global rust-resistance response. To identify markers linked to the resistance locus, screening of RAPD polymorphisms was conducted using bulked segregant analysis in a large full-sib family. A linkage group was built around the Ppr1 gene (P. psidii resistance gene 1) encompassing six RAPD markers, with a genetic window spanning 5 cM with the two mostclosely linked flanking markers. Besides these two flanking markers, RAPD marker AT9/917 co-segregated with Ppr1 without a single recombinant in 994 meioses. This tightly linked marker should prove useful for marker-assisted introgression and will provide an initial lead for a positional cloning effort of this resistance allele. This is the first report of a disease resistance gene identified in Eucalyptus, and one of the few examples of the involvement of a major gene in a non-coevolved pathosystem. |
dc.description.version.pt_BR.fl_txt_mv |
Sim |
dc.description.status.pt_BR.fl_txt_mv |
Publicado |
description |
Rust is one of the most-damaging eucalypt diseases in Brazil and is considered a potential threat to eucalypt plantations worldwide. To determine the mode of inheritance of esistance in the Eucalyptus grandis—Puccinia psidii pathosystem, ten full-sib families, generated from crosses between susceptible and resistant trees, were inoculated with a single-pustule isolate of the pathogen and rust severity was scored. The observed segregation ratios in segregating families suggested major gene control of rust resistance, although clearly incomplete penetrance, variable expressivity and minor genes are also involved in the global rust-resistance response. To identify markers linked to the resistance locus, screening of RAPD polymorphisms was conducted using bulked segregant analysis in a large full-sib family. A linkage group was built around the Ppr1 gene (P. psidii resistance gene 1) encompassing six RAPD markers, with a genetic window spanning 5 cM with the two mostclosely linked flanking markers. Besides these two flanking markers, RAPD marker AT9/917 co-segregated with Ppr1 without a single recombinant in 994 meioses. This tightly linked marker should prove useful for marker-assisted introgression and will provide an initial lead for a positional cloning effort of this resistance allele. This is the first report of a disease resistance gene identified in Eucalyptus, and one of the few examples of the involvement of a major gene in a non-coevolved pathosystem. |
publishDate |
2003 |
dc.date.issued.fl_str_mv |
2003 |
dc.date.accessioned.fl_str_mv |
2016-10-10T03:52:34Z |
dc.date.available.fl_str_mv |
2016-10-10T03:52:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
status_str |
publishedVersion |
format |
article |
dc.identifier.citation.fl_str_mv |
JUNGHANS, Davi Theodoro et al. Resistance to rust (Puccinia psidii winter) in eucalyptus: mode of inheritance and mapping of a major gene with RAPD markers. Theor Appl Genet, v.108, p.175-180, 2003. |
dc.identifier.uri.fl_str_mv |
http://twingo.ucb.br:8080/jspui/handle/10869/521 https://repositorio.ucb.br:9443/jspui/handle/123456789/7755 |
identifier_str_mv |
JUNGHANS, Davi Theodoro et al. Resistance to rust (Puccinia psidii winter) in eucalyptus: mode of inheritance and mapping of a major gene with RAPD markers. Theor Appl Genet, v.108, p.175-180, 2003. |
url |
http://twingo.ucb.br:8080/jspui/handle/10869/521 https://repositorio.ucb.br:9443/jspui/handle/123456789/7755 |
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por |
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