A rat pancreatic ribonuclease fused to a late cotton pollen promoter severely reduces pollen viability in tobacco plants

Detalhes bibliográficos
Autor(a) principal: Souza, Regina Beatriz Bernd
Data de Publicação: 2000
Outros Autores: Grossi-de-Sá, Maria Fátima, Ellis, David D., McCown, Brent H.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UCB
Texto Completo: http://twingo.ucb.br:8080/jspui/handle/10869/536
https://repositorio.ucb.br:9443/jspui/handle/123456789/7685
Resumo: The effects of an animal RNase fused to the late cotton pollen-specific promoter G9 in a plant system were investigated. Expression of the chimeric genes G9-uidA and G9-RNase in tobacco plants showed that the 1.2-kb promoter fragment of the G9 gene was sufficient to maintain tissue and temporal specificity in a heterologous system. GUS (beta-glucuronidase) expression was detected only in pollen from anther stage 6 through anthesis, with maximal GUS activity in pollen from stage 10 anthers. Investigating the effects of the rat RNase on pollen viability at stage 10, we found that pollen viability was reduced from 79 to 8% and from 89 to 40%, in pollen germination and fluoresceine diacetate assays, respectively, in one G9-RNase transgenic line, suggesting a lethal effect of the RNase gene. This indicates that the rat RNase produces deleterious effects in this plant system and may be useful for engineering male sterility.
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spelling Souza, Regina Beatriz BerndGrossi-de-Sá, Maria FátimaEllis, David D.McCown, Brent H.2016-10-10T03:52:21Z2016-10-10T03:52:21Z2000SOUZA, Regina Beatriz Bernd et. al. A rat pancreatic ribonuclease fused to a late cotton pollen promoter severely reduces pollen viability in tobacco plants. Genetics and Molecular Biology, v. 23, n. 2, p. 435-443, 2000.http://twingo.ucb.br:8080/jspui/handle/10869/536https://repositorio.ucb.br:9443/jspui/handle/123456789/7685The effects of an animal RNase fused to the late cotton pollen-specific promoter G9 in a plant system were investigated. Expression of the chimeric genes G9-uidA and G9-RNase in tobacco plants showed that the 1.2-kb promoter fragment of the G9 gene was sufficient to maintain tissue and temporal specificity in a heterologous system. GUS (beta-glucuronidase) expression was detected only in pollen from anther stage 6 through anthesis, with maximal GUS activity in pollen from stage 10 anthers. Investigating the effects of the rat RNase on pollen viability at stage 10, we found that pollen viability was reduced from 79 to 8% and from 89 to 40%, in pollen germination and fluoresceine diacetate assays, respectively, in one G9-RNase transgenic line, suggesting a lethal effect of the RNase gene. This indicates that the rat RNase produces deleterious effects in this plant system and may be useful for engineering male sterility.Made available in DSpace on 2016-10-10T03:52:21Z (GMT). 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dc.title.pt_BR.fl_str_mv A rat pancreatic ribonuclease fused to a late cotton pollen promoter severely reduces pollen viability in tobacco plants
title A rat pancreatic ribonuclease fused to a late cotton pollen promoter severely reduces pollen viability in tobacco plants
spellingShingle A rat pancreatic ribonuclease fused to a late cotton pollen promoter severely reduces pollen viability in tobacco plants
Souza, Regina Beatriz Bernd
title_short A rat pancreatic ribonuclease fused to a late cotton pollen promoter severely reduces pollen viability in tobacco plants
title_full A rat pancreatic ribonuclease fused to a late cotton pollen promoter severely reduces pollen viability in tobacco plants
title_fullStr A rat pancreatic ribonuclease fused to a late cotton pollen promoter severely reduces pollen viability in tobacco plants
title_full_unstemmed A rat pancreatic ribonuclease fused to a late cotton pollen promoter severely reduces pollen viability in tobacco plants
title_sort A rat pancreatic ribonuclease fused to a late cotton pollen promoter severely reduces pollen viability in tobacco plants
author Souza, Regina Beatriz Bernd
author_facet Souza, Regina Beatriz Bernd
Grossi-de-Sá, Maria Fátima
Ellis, David D.
McCown, Brent H.
author_role author
author2 Grossi-de-Sá, Maria Fátima
Ellis, David D.
McCown, Brent H.
author2_role author
author
author
dc.contributor.author.fl_str_mv Souza, Regina Beatriz Bernd
Grossi-de-Sá, Maria Fátima
Ellis, David D.
McCown, Brent H.
dc.description.abstract.por.fl_txt_mv The effects of an animal RNase fused to the late cotton pollen-specific promoter G9 in a plant system were investigated. Expression of the chimeric genes G9-uidA and G9-RNase in tobacco plants showed that the 1.2-kb promoter fragment of the G9 gene was sufficient to maintain tissue and temporal specificity in a heterologous system. GUS (beta-glucuronidase) expression was detected only in pollen from anther stage 6 through anthesis, with maximal GUS activity in pollen from stage 10 anthers. Investigating the effects of the rat RNase on pollen viability at stage 10, we found that pollen viability was reduced from 79 to 8% and from 89 to 40%, in pollen germination and fluoresceine diacetate assays, respectively, in one G9-RNase transgenic line, suggesting a lethal effect of the RNase gene. This indicates that the rat RNase produces deleterious effects in this plant system and may be useful for engineering male sterility.
dc.description.status.pt_BR.fl_txt_mv Publicado
description The effects of an animal RNase fused to the late cotton pollen-specific promoter G9 in a plant system were investigated. Expression of the chimeric genes G9-uidA and G9-RNase in tobacco plants showed that the 1.2-kb promoter fragment of the G9 gene was sufficient to maintain tissue and temporal specificity in a heterologous system. GUS (beta-glucuronidase) expression was detected only in pollen from anther stage 6 through anthesis, with maximal GUS activity in pollen from stage 10 anthers. Investigating the effects of the rat RNase on pollen viability at stage 10, we found that pollen viability was reduced from 79 to 8% and from 89 to 40%, in pollen germination and fluoresceine diacetate assays, respectively, in one G9-RNase transgenic line, suggesting a lethal effect of the RNase gene. This indicates that the rat RNase produces deleterious effects in this plant system and may be useful for engineering male sterility.
publishDate 2000
dc.date.issued.fl_str_mv 2000
dc.date.accessioned.fl_str_mv 2016-10-10T03:52:21Z
dc.date.available.fl_str_mv 2016-10-10T03:52:21Z
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dc.identifier.citation.fl_str_mv SOUZA, Regina Beatriz Bernd et. al. A rat pancreatic ribonuclease fused to a late cotton pollen promoter severely reduces pollen viability in tobacco plants. Genetics and Molecular Biology, v. 23, n. 2, p. 435-443, 2000.
dc.identifier.uri.fl_str_mv http://twingo.ucb.br:8080/jspui/handle/10869/536
https://repositorio.ucb.br:9443/jspui/handle/123456789/7685
identifier_str_mv SOUZA, Regina Beatriz Bernd et. al. A rat pancreatic ribonuclease fused to a late cotton pollen promoter severely reduces pollen viability in tobacco plants. Genetics and Molecular Biology, v. 23, n. 2, p. 435-443, 2000.
url http://twingo.ucb.br:8080/jspui/handle/10869/536
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